5-HT1A modulator 1

Binding is determined using membranes prepared from bovine hippocampus. The receptor is labeled with 0.5 nM [³H]-8-hydroxydipropylaminotetralin (8-OH-DPAT) by incubation at 25°C for 30 min with 11 concentrations of the test compounds (1-10⁵ nM). Nonspecific binding is determined using 10^-5 Mbuspirone. Competition experiments are analyzed using the iterative nonlinear least-squares curve-fitting program Inplot 4, graphpad; IC₅₀ values are calculated using the Cheng-Prusoff equation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Rats^[1]
Wistar rats (n=6) are used. 5-HT1A modulator 1 is tested at pharmacological doses (1 and 2 mg/kg ip, respectively) and at high doses (32 and 64 mg/kg ip) in rats. The intensity of forepaw treading is expressed as percentage of the maximal possible score. The 5HT_1A agonist 8-OH-DPAT induces forepaw treading and is used as a reference compound.
Mice^[1]
Swiss mice are injected with the test compound (e.g., 5-HT1A modulator 1, 0.25 and 1 mg/kg ip) before an injection of 5HTP (400 mg/kg ip). The number of head twiches occurring in a 10 min period starting 10 min after the injection of 5HTP is counted. Cyproheptadine is used as reference compound.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.