ナイシン
Based on 3 publication(s) in Google Scholar
Nisin is a bacteriocin produced by a group of Gram-positive bacteria that belongs to Lactococcus and Streptococcus species. Nisin has antibacterial and anti-inflammatory activity.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 1414-45-5
- 分子式: C143H230N42O37S7
- 分子量:3354.07
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保管条件:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 Nisin
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Customer Validation & Images
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Cell Proliferation/Viability Assay
Antibiotic アイソフォーム固有の製品をすべて表示
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生物活性
Nisin (100-800 μg/mL, 36 h) inhibits HNSCC cell ballooning[1].
Nisin (500-7000 IU/mL, 24 h) induces free-living amoeba acanthamoeba cell cycle arrest[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A. castellanii trophozoites
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Concentration:500, 1000, 2000, 3000, 4000, 6000, 7000 IU/mL
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Incubation Time:24, 72 h
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Result:Inhibited amebic growth in a concentration-dependent manner at 24 h.
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Cell Line:A. castellanii trophozoites
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Concentration:7000 IU/mL
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Incubation Time:24 h
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Result:Increased S phase to 13.3% and the percentage of G1 to 11.1%.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Rat uterine endometritis model[3]
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Dosage:25 mg/kg
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Administration:i.p. for 7 days
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Result:Decreased the endometritis-induced increase in uterine weight, restored endometrial architecture.
Improved levels of B7-2, IFN-γ, IL-2 and IL-8.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
化学情報
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CAS 番号 1414-45-5
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性状 Solid
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分子量 3354.07
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分子式 C143H230N42O37S7
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Color Off-white to light brown
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別名
Nisin; Nisin A
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配列
Ile-Dhb-Ala-Ile-Dha-Leu-Ala-Abu-Pro-Gly-Ala-Lys-Abu-Gly-Ala-Leu-Met-Gly-Ala-Asn-Met-Lys-Abu-Ala-Aba-Ala-His-Ala-Ser-Ile-His-Val-Dha-Lys (Sulfide bridge: Ala3-Ala7, Abu8-Ala11, Abu13-Ala19, Abu23-Ala26, Aba25-Ala28)
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シーケンスの短縮
I-Dhb-AI-Dha-LA-Abu-PGAK-Abu-GALMGANMK-Abu-A-Aba-AHASIHV-Dha-K (Sulfide bridge: Ala3-Ala7, Abu8-Ala11, Abu13-Ala19, Abu23-Ala26, Aba25-Ala28)
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (3)
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Journal Impact Factor
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Most Recent
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J Hazard Mater
Development of transcriptional factor-based whole-cell biosensors to monitor and degrade antibiotics using mutant cells obtained via adaptive laboratory evolution. [Abstract]2024 Jul 15:473:134536. PMID: 38759406
Nisin purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2024 Jul 15:473:134536. [Abstract]
Nisin (6-60 nM) greatly diminished growth and biosensor performance of L. lactis NZ9000.
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Vaccine
Assessment of recombinant Lactobacillus vector-based expressing IFITM3 protein anti-PRV infection in mice. [Abstract]2025 Apr 16:54:127093. PMID: 40245770 -
溶剤 & 溶解度
H2O : 6.67 mg/mL (1.99 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
純度とドキュメンテーション
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データシート (301 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Shin JM, et al. Biomedical applications of nisin. J Appl Microbiol. 2016 Jun;120(6):1449-65. [Content Brief]
[2]. de Carvalho Clímaco M, et al. Nisin Induces Cell-Cycle Arrest in Free-Living Amoebae Acanthamoeba castellanii. Acta Parasitol. 2022 Mar;67(1):511-517. [Content Brief]
[3]. Jia Z, et al. Nisin reduces uterine inflammation in rats by modulating concentrations of pro- and anti-inflammatory cytokines. Am J Reprod Immunol. 2019 May;81(5):e13096. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.2981 mL | 1.4907 mL | 2.9815 mL | 7.4536 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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