AF-45
AF-45 inhibits IRAK4 and IRAK1, with IC50s of 128 nM and 1765 nM. AF-45 inhibits the release of IL-6 and TNF-α in macrophages, with IC50s of 0.53-1.54 μM and 0.6-2.75 μM. AF-45 is also an inhibitor for NF-κB/MAPK signaling pathway. AF-45 exhibits anti-inflammatory activities against DSS-induced ulcerative colitis and Lipopolysaccharide (HY-D1056)-induced acute lung injury in mouse model. AF-45 exhibits good pharmacokinetic characteristics in rat models.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 分子式: C22H25N3O3
- 分子量:379.45
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
|
IRAK4 128 nM (IC50) |
IRAK1 1765 nM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| J774.A1 | IC50 |
1.54 μM
Compound: d5; AF-45
|
Antiinflammatory activity in LPS-induced mouse J774.A1 cells assessed as inhibition of IL-6 release pretreated for 30 mins followed by LPS stimulation and measured after 24 hrs by ELISA
Antiinflammatory activity in LPS-induced mouse J774.A1 cells assessed as inhibition of IL-6 release pretreated for 30 mins followed by LPS stimulation and measured after 24 hrs by ELISA
|
[PMID: 38913701] |
| J774.A1 | IC50 |
2.75 μM
Compound: d5; AF-45
|
Antiinflammatory activity in LPS-induced mouse J774.A1 cells assessed as inhibition of TNF-alpha release pretreated for 30 mins followed by LPS stimulation and measured after 24 hrs by ELISA
Antiinflammatory activity in LPS-induced mouse J774.A1 cells assessed as inhibition of TNF-alpha release pretreated for 30 mins followed by LPS stimulation and measured after 24 hrs by ELISA
|
[PMID: 38913701] |
| RAW264.7 | IC50 |
1.27 μM
Compound: d5; AF-45
|
Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as inhibition of IL-6 release pretreated for 30 mins followed by LPS stimulation and measured after 24 hrs by ELISA
Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as inhibition of IL-6 release pretreated for 30 mins followed by LPS stimulation and measured after 24 hrs by ELISA
|
[PMID: 38913701] |
| RAW264.7 | IC50 |
2.6 μM
Compound: d5; AF-45
|
Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as inhibition of TNF-alpha release pretreated for 30 mins followed by LPS stimulation and measured after 24 hrs by ELISA
Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as inhibition of TNF-alpha release pretreated for 30 mins followed by LPS stimulation and measured after 24 hrs by ELISA
|
[PMID: 38913701] |
| THP-1 | IC50 |
0.53 μM
Compound: d5; AF-45
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Antiinflammatory activity in LPS-induced human THP-1 cells assessed as inhibition of IL-6 release pretreated for 30 mins followed by LPS stimulation and measured after 24 hrs by ELISA
Antiinflammatory activity in LPS-induced human THP-1 cells assessed as inhibition of IL-6 release pretreated for 30 mins followed by LPS stimulation and measured after 24 hrs by ELISA
|
[PMID: 38913701] |
| THP-1 | IC50 |
0.6 μM
Compound: d5; AF-45
|
Antiinflammatory activity in LPS-induced human THP-1 cells assessed as inhibition of TNF-alpha release pretreated for 30 mins followed by LPS stimulation and measured after 24 hrs by ELISA
Antiinflammatory activity in LPS-induced human THP-1 cells assessed as inhibition of TNF-alpha release pretreated for 30 mins followed by LPS stimulation and measured after 24 hrs by ELISA
|
[PMID: 38913701] |
化学情報
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分子量 379.45
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分子式 C22H25N3O3
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SMILES
COC1=CC2=C(N=CN=C2O[C@H]3CC[C@@H](N)CC3)C=C1OCC4=CC=CC=C4
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
AF-45 関連分類
HY-162641 関連分類
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