AZT triphosphate
AZT triphosphate (3'-Azido-3'-deoxythymidine-5'-triphosphate) is a active triphosphate metabolite of Zidovudine (AZT). AZT triphosphate exhibits antiretroviral activity and inhibits replication of HIV. AZT triphosphate also inhibits the DNA polymerase of HBV. AZT triphosphate activates the mitochondria-mediated apoptosis pathway.
The free form of the compound is prone to instability, it is advisable to consider the stable salt form (AZT triphosphate tetraammonium) that retains the same biological activity.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 92586-35-1
- 分子式: C10H16N5O13P3
- 分子量:507.18
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Other In-stock Forms of AZT triphosphate
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
製品説明
IC50 & Target
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DNA Polymerase |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| H9 | IC50 |
0.022 μM
Compound: 2
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Compound concentration that causes 50 % inhibition of HIV-1 peptide-derived reverse transcriptase (RT) obtained from HIV-1-infected H9 cell cultures
Compound concentration that causes 50 % inhibition of HIV-1 peptide-derived reverse transcriptase (RT) obtained from HIV-1-infected H9 cell cultures
|
10.1016/S0960-894X(01)80472-8 |
| HFF | IC50 |
22.1 μM
Compound: AZT-TP
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Inhibition of HCMV DNA polymerase infected in HFF cells
Inhibition of HCMV DNA polymerase infected in HFF cells
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[PMID: 20403696] |
| HFF | IC50 |
5.8 μM
Compound: AZT-TP
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Inhibition of VZV KOS DNA polymerase infected in HFF cells
Inhibition of VZV KOS DNA polymerase infected in HFF cells
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[PMID: 20403696] |
| Sf9 | IC50 |
22100 nM
Compound: AZT-TP
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Inhibition of HCMV recombinant DNA polymerase expressed in baculovirus infected SF9 cells after 12 mins by scintillation proximity assay
Inhibition of HCMV recombinant DNA polymerase expressed in baculovirus infected SF9 cells after 12 mins by scintillation proximity assay
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[PMID: 20167488] |
| Sf9 | IC50 |
5800 nM
Compound: AZT-TP
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Inhibition of Varicella zoster virus recombinant DNA polymerase expressed in baculovirus infected SF9 cells after 12 mins by scintillation proximity assay
Inhibition of Varicella zoster virus recombinant DNA polymerase expressed in baculovirus infected SF9 cells after 12 mins by scintillation proximity assay
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[PMID: 20167488] |
| Vero | IC50 |
3.3 μM
Compound: AZT-TP
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Inhibition of HSV1 KOS DNA polymerase infected in african green monkey Vero cells
Inhibition of HSV1 KOS DNA polymerase infected in african green monkey Vero cells
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[PMID: 20403696] |
体外実験
AZT triphosphate (3'-Azido-3'-deoxythymidine-5'-triphosphate) accumulation disrupts the mitochondrial tubular network in H9c2 cells through Treatment with 100 μM Zidovudine (AZT) for 48h. AZT triphosphate accumulation causes downregulation of Opa1 and upregulation of Drp1. AZT triphosphate causes mitochondrial dysfunction, increases the production of cytotoxic reactive oxygen species (ROS), and impairs the balance of the mitochondrial quality control system in H9c2 cell model established from rat embryonic myoblasts[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 92586-35-1
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分子量 507.18
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分子式 C10H16N5O13P3
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SMILES
O=C1NC(N([C@@H](C[C@@H]2N=[N+]=[N-])O[C@@H]2COP(OP(O)(OP(O)(O)=O)=O)(O)=O)C=C1C)=O
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別名
3'-Azido-3'-deoxythymidine-5'-triphosphate
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
[1]. Ryosuke Nomura, et al. Azidothymidine-triphosphate Impairs Mitochondrial Dynamics by Disrupting the Quality Control System. Redox Biol. 2017 Oct;13:407-417. [Content Brief]
[2]. Takeya Sato, et al. Engineered Human tmpk/AZT as a Novel Enzyme/Prodrug Axis for Suicide Gene Therapy. Mol Ther. 2007 May;15(5):962-70. [Content Brief]
[3]. K Y Hostetler, et al. Enhanced Oral Absorption and Antiviral Activity of 1-O-octadecyl-sn-glycero-3-phospho-acyclovir and Related Compounds in Hepatitis B Virus Infection, in Vitro. Biochem Pharmacol. 1997 Jun 15;53(12):1815-22. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
AZT triphosphate 関連分類
HY-116364 関連分類
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