EP-459

ICR mice (male, seven-week-old)^[1]

500 μg

i.p.; daily; 8 consecutive days

Measured proline aminopeptidase activity at 0.85 nmol per min per mg protein, formyl methionine aminopeptidase activity at 4.21 nmol per min per mg protein, phenylalanine aminopeptidase activity at 3.86 nmol per min per mg protein, Gly-Pro-Leu-AP activity at 0.55 nmol per min per mg protein, α-D-mannosidase activity at 0.17 nmol per min per mg protein, N-Ac-β-D-glucosaminidase activity at 0.42 nmol per min per mg protein, esterase activity at 34.59 nmol per min per mg protein, AP-A activity at 0.44 nmol per min per mg protein, AP-B activity at 5.51 nmol per min per mg protein, leucine aminopeptidase activity at 3.25 nmol per min per mg protein, trypsin-like enzyme activity at 18.05 nmol per min per mg protein, chymotrypsin-like enzyme activity at 21.37 nmol per min per mg protein, elastase-like enzyme activity at 17.77 nmol per min per mg protein, cathepsin C activity at 0.42 nmol per min per mg protein, α-D-glucosidase activity at 0.51 nmol per min per mg protein, creatine kinase activity at 27.24 nmol per min per mg protein, and phosphatase activity at 1.63 nmol per min per mg protein in forelimb muscle.
Measured cathepsin C activity at 2.40 nmol per min per mg protein, N-Ac-β-D-glucosaminidase activity at 0.56 nmol per min per mg protein, elastase-like enzyme activity at 25.80 nmol per min per mg protein, AP-A activity at 4.93 nmol per min per mg protein, AP-B activity at 7.09 nmol per min per mg protein, proline aminopeptidase activity at 1.54 nmol per min per mg protein, leucine aminopeptidase activity at 7.41 nmol per min per mg protein, formyl methionine aminopeptidase activity at 10.09 nmol per min per mg protein, phenylalanine aminopeptidase activity at 7.33 nmol per min per mg protein, Gly-Pro-Leu-AP activity at 3.24 nmol per min per mg protein, trypsin-like enzyme activity at 16.35 nmol per min per mg protein, chymotrypsin-like enzyme activity at 22.68 nmol per min per mg protein, α-D-glucosidase activity at 1.74 nmol per min per mg protein, α-D-mannosidase activity at 1.45 nmol per min per mg protein, phosphatase activity at 3.29 nmol per min per mg protein, and esterase activity at 944 nmol per min per mg protein in liver.
Clustered closely with E-64C and clearly differentiated from leupeptin and its analogues in in vivo enzymatic activity changes across all six tested organs via multivariate principal component analysis.