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製品番号: HY-170983

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MC-25B

MC-25B is a DCAF16-recruiting FKBP12 PROTAC degrader with a DC₅₀ of 0.35 μM. MC-25B promotes the formation of a ternary complex with DCAF16 and nuclear-localized FKBP12_NLS, thereby mediating DCAF16-dependent degradation of FKBP12_NLS via the proteasome and Cullin-RING ligase pathway.
(Pink: FKBP12 ligand (HY-170988); Blue: DCAF16 ligand (HY-170986); Black: linker (HY-128844)).

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

分子式: C₆₅H₉₂ClN₇O₁₄
分子量:1230.92

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (268 KB)

MC-25B 構造式

容量

価格（税別）

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数量

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

MC-25B is a DCAF16-recruiting FKBP12 PROTAC degrader with a DC₅₀ of 0.35 μM. MC-25B promotes the formation of a ternary complex with DCAF16 and nuclear-localized FKBP12_NLS, thereby mediating DCAF16-dependent degradation of FKBP12_NLS via the proteasome and Cullin-RING ligase pathway^[1]. (Pink: FKBP12 ligand (HY-170988); Blue: DCAF16 ligand (HY-170986); Black: linker (HY-128844)).

IC₅₀ & Target^[1]

FKBP12

0.35 μM (DC₅₀)

体外実験

MC-25B (1 μM; 24 h) induces selective, DCAF16-dependent degradation of FKBP12 in HEK293T cells expressing FLAG-FKBP12_NLS, with only one identified DCAF16-independent off-target effect^[1].
MC-25B (0.1-5 μM; 8 h) induces dose-dependent degradation of FLAG-FKBP12_NLS in HEK293T cells overexpressing HA-DCAF16, with a DC₅₀ of 0.35 μM and a Dmax of 89%^[1].
MC-25B (1-2 μM; 8 h) induces the degradation of FLAG-FKBP12_NLS in HEK293T cells expressing the DCAF16_3Cys mutant (retaining only C177−179), confirming that this cysteine cluster plays a critical role in MC-25B-mediated degradation^[1].
MC-25B (incubated for 72 h) inhibits the viability of HEK293T cells, with an IC₅₀ value of 2.2 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

HEK293T cells overexpressing HA-DCAF16 and FLAG-FKBP12_NLS
Concentration:

0.1 μM, 0.2 μM, 0.5 μM, 1 μM, 2 μM, 5 μM
Incubation Time:

8 h
Result:

Induced dose-dependent degradation of FLAG-FKBP12_NLS, with a maximum degradation (D_max) of 89% and a half-maximal degradation concentration (DC₅₀) of 0.35 μM.

化学情報

分子量 1230.92
分子式 C₆₅H₉₂ClN₇O₁₄
SMILES

O=C(CN1CCN(C2=CC=CC(C(N3CCC(N(C4CC4)C(CCl)=O)CC3)=O)=C2)CC1)NCCCOCCOCCOCCCNC(COC5=CC=CC([C@H](OC([C@@H]6CCCCN6C(C(C(C)(C)CC)=O)=O)=O)CCC7=CC(OC)=C(OC)C=C7)=C5)=O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (268 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Campos MA, et al. Discovery of DCAF16 Binders for Targeted Protein Degradation. ACS chemical biology. 2025 Feb 21;20(2):479-488. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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