Muramyl dipeptide
Based on 11 publication(s) in Google Scholar
Muramyl dipeptide (MDP) is a synthetic immunoreactive peptide, consisting of N-acetyl muramic acid attached to a short amino acid chain of L-Ala-D-isoGln. Muramyl dipeptide is an inducer of bone formation through induction of Runx2. Muramyl dipeptide directly enhances osteoblast differentiation by up-regulating Runx2 gene expression through MAPK pathways. Muramyl dipeptide is a NLRP1 agonist.
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- 純度: 99.07%
- CAS 番号: 53678-77-6
- 分子式: C19H32N4O11
- 分子量:492.48
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保管条件:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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- 純度とドキュメンテーション
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MedChemExpress（MCE）の使用を引用している文献 Muramyl dipeptide
More- Nat Commun. 2025 Jul 22;16(1):6737. [Abstract]
- Sci Immunol. 2026 Apr 3;11(118):eaeb6484. [Abstract]
- J Exp Med. 2026 Feb 2;223(2):e20251051. [Abstract]
- Cell Chem Biol. 2026 Feb 9:S2451-9456(26)00025-5. [Abstract]
- J Ethnopharmacol. 2026 Jan 10:354:120514. [Abstract]
- Int Immunopharmacol. 2024 Nov 16;143(Pt 3):113622. [Abstract]
- Sci Rep. 2025 Oct 8;15(1):35192. [Abstract]
- J Bacteriol. 2020 Sep 23;202(20):e00689-19. [Abstract]
- Mol Cell Biol. 2024;44(10):411-428. [Abstract]
- bioRxiv. 2025 Nov 11.
- Research Square Preprint. 2023 Aug 8.
Customer Validation & Images
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WB
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IF
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WB
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ELISA
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IF
生物活性
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p38 MAPK |
NLRP1 |
Muramyl dipeptide (0.1-10 μg/mL; 24 hours) increases protein expression of Runx2 in a dose-dependent manner[1].
Muramyl dipeptide (0.1-10 μg/mL; 6 hours) increases mRNA levels of Runx2 in a dose-dependent manner[1].
Muramyl dipeptide indirectly attenuates osteoclast differentiation through a decreased RANKL/OPG ratio[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MC3T3-E1 cells
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Concentration:0.1, 1, 10 µg/mL
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Incubation Time:24 hours
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Result:Increased protein expression of Runx2 in a dose-dependent manner.
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Cell Line:MC3T3-E1 cells
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Concentration:0.1, 1, 10 µg/mL
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Incubation Time:6 hours
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Result:Increases mRNA levels of Runx2 in a dose-dependent manner.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:RANKL-induced osteoporosis model (Five-week-old C57BL/6 mice)[1]
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Dosage:1.25 mg/kg
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Administration:i.p.; twice (RANKL-induced osteoporosis for 3 weeks and euthanized at 4 weeks)
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Result:Significantly enhanced the trabecular bone volume (BV/TV) and trabecular number (Tb.N).
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
化学情報
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CAS 番号 53678-77-6
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性状 Solid
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分子量 492.48
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分子式 C19H32N4O11
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Color White to off-white
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SMILES
C[C@@H](C(N[C@@H](C(N)=O)CCC(O)=O)=O)NC([C@H](O[C@H]1[C@@H]([C@H](OC([C@@H]1NC(C)=O)O)CO)O)C)=O
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別名
MDP; アジュバントペプチド
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (11)
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Journal Impact Factor
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Most Recent
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Nat Commun
Candida albicans Ssy1 is the extracellular sensor of gut microbiota-derived peptidoglycan fragments mediating invasive hyphal growth in the host. [Abstract]2025 Jul 22;16(1):6737. PMID: 40695824 -
Sci Immunol
2026 Apr 3;11(118):eaeb6484. PMID: 41931597 -
J Exp Med
ERAdP facilitates biogenesis of dense core vesicles in Paneth cells to enhance intestinal defense. [Abstract]2026 Feb 2;223(2):e20251051. PMID: 41474967 -
Cell Chem Biol
Discovery of bioactive peptidoglycan fragments from Lactobacillaceae that confer intestinal protection in hosts. [Abstract]2026 Feb 9:S2451-9456(26)00025-5. PMID: 41666925 -
J Ethnopharmacol
Pilose antler peptide enhances diabetic fracture healing by modulating the CREB-Smad2/3-Runx2 signaling axis. [Abstract]2026 Jan 10:354:120514. PMID: 40889600
Muramyl dipeptide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Jan 10:354:120514. [Abstract]
Western blot analysis of p-Smad3, Runx2, OPN, and OCN protein levels and quantification. Runx2 agonist Muramyl dipeptide (MDP) (10 μg/mL; 24 h) partially restored osteogenic marker expression in Smad2/3-overexpressed MC3T3-E1 cells.
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Int Immunopharmacol
VCPIP1 negatively regulates NF-κB signaling pathways by deubiquitinating and stabilizing Erbin in MDP-stimulated macrophages. [Abstract]2024 Nov 16;143(Pt 3):113622. PMID: 39550842
Muramyl dipeptide purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 16;143(Pt 3):113622. [Abstract]
Muramyl dipeptide (MDP) (10 μg/ml, 1 h) enhanced the colocalization of VCPIP1 and Erbin in RAW264.7 cells.
Muramyl dipeptide purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 16;143(Pt 3):113622. [Abstract]
Muramyl dipeptide (MDP) (10 μg/ml, 1 h) treatment increased the protein expression of VCPIP1, Erbin, and the phosphorylation levels of IKKα/β, IκBα, and NF-κB in RAW264.7 cells.
Muramyl dipeptide purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 16;143(Pt 3):113622. [Abstract]
Muramyl dipeptide (MDP) (10 μg/mL, 6 h) significantly increased levels of inflammatory factors TNF-α, IL-6, and IL-1β in the cultured supernatant of RAW264.7 cells.
Muramyl dipeptide purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 16;143(Pt 3):113622. [Abstract]
Muramyl dipeptide (MDP) (10 μg/ml, 1 h) induced increased nuclear p65 in both BMDMs from VCPIP1 KO and WT mice.
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Sci Rep
Probenecid relieves LPS-induced neuroinflammation by modulating the NLRP1 inflammasome pathway in BV2 cells. [Abstract]2025 Oct 8;15(1):35192. PMID: 41062518 -
J Bacteriol
NOD2/c-Jun NH2-Terminal Kinase Triggers Mycoplasma ovipneumoniae-Induced Macrophage Autophagy. [Abstract]2020 Sep 23;202(20):e00689-19. PMID: 32778560 -
Mol Cell Biol
Unveiling the Role of Sik1 in Osteoblast Differentiation: Implications for Osteoarthritis. [Abstract]2024;44(10):411-428. PMID: 39169784 -
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溶剤 & 溶解度
DMSO : 100 mg/mL (203.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (101.53 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 5 mg/mL (10.15 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 5 mg/mL (10.15 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
純度とドキュメンテーション
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データシート (277 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Park OJ, et al. Muramyl Dipeptide, a Shared Structural Motif of Peptidoglycans, Is a Novel Inducer of Bone Formation through Induction of Runx2. J Bone Miner Res. 2017 Jul;32(7):1455-1468. [Content Brief]
[2]. V Kaushal, et al. Neuronal NLRP1 inflammasome activation of Caspase-1 coordinately regulates inflammatory interleukin-1-beta production and axonal degeneration-associated Caspase-6 activation. Cell Death Differ. 2015 Oct;22(10):1676-86. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 2.0305 mL | 10.1527 mL | 20.3054 mL | 50.7635 mL |
| 5 mM | 0.4061 mL | 2.0305 mL | 4.0611 mL | 10.1527 mL | |
| 10 mM | 0.2031 mL | 1.0153 mL | 2.0305 mL | 5.0763 mL | |
| 15 mM | 0.1354 mL | 0.6768 mL | 1.3537 mL | 3.3842 mL | |
| 20 mM | 0.1015 mL | 0.5076 mL | 1.0153 mL | 2.5382 mL | |
| 25 mM | 0.0812 mL | 0.4061 mL | 0.8122 mL | 2.0305 mL | |
| 30 mM | 0.0677 mL | 0.3384 mL | 0.6768 mL | 1.6921 mL | |
| 40 mM | 0.0508 mL | 0.2538 mL | 0.5076 mL | 1.2691 mL | |
| 50 mM | 0.0406 mL | 0.2031 mL | 0.4061 mL | 1.0153 mL | |
| 60 mM | 0.0338 mL | 0.1692 mL | 0.3384 mL | 0.8461 mL | |
| 80 mM | 0.0254 mL | 0.1269 mL | 0.2538 mL | 0.6345 mL | |
| 100 mM | 0.0203 mL | 0.1015 mL | 0.2031 mL | 0.5076 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Muramyl dipeptide 関連分類
HY-127090 関連分類
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