STAT3-IN-13
Based on 1 publication(s) in Google Scholar
STAT3-IN-13 (compound 6f) is a potent STAT3 inhibitor. STAT3-IN-13 has anti-proliferative effects and binds to the STAT3 SH2 domain with a KD of 0.46 μM. STAT3-IN-13 inhibits the phosphorylation of STAT3 Y705 and downstream target gene expression. STAT3-IN-13 induces apoptosis in vitro and suppresses the growth and metastasis of tumor in vivo. STAT3-IN-13 can be used for cancer research.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.24%
- CAS 番号: 2248552-86-3
- 分子式: C21H20N6O3S
- 分子量:436.49
-
保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 STAT3-IN-13
More
生物活性
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| 143B | IC50 |
0.19 μM
Compound: 6f
|
Antiinvasive activity against human 143B cells assessed as reduction in cell invasion measured after 12 hrs by crystal violet staining based inverted microscopic analysis
Antiinvasive activity against human 143B cells assessed as reduction in cell invasion measured after 12 hrs by crystal violet staining based inverted microscopic analysis
|
[PMID: 35476936] |
| 143B | IC50 |
0.25 μM
Compound: 6f
|
Antiproliferative activity against human 143B cells assessed as inhibition of cell growth incubated for 48 hrs by cell titer based MTS assay
Antiproliferative activity against human 143B cells assessed as inhibition of cell growth incubated for 48 hrs by cell titer based MTS assay
|
[PMID: 35476936] |
| 143B | IC50 |
0.48 μM
Compound: 6f
|
Antimigratory activity against human 143B cells assessed as reduction in cell migration measured after 12 hrs by wound healing assay
Antimigratory activity against human 143B cells assessed as reduction in cell migration measured after 12 hrs by wound healing assay
|
[PMID: 35476936] |
| HOS | IC50 |
0.11 μM
Compound: 6f
|
Antiproliferative activity against human HOS cells assessed as inhibition of cell growth incubated for 48 hrs by cell titer based MTS assay
Antiproliferative activity against human HOS cells assessed as inhibition of cell growth incubated for 48 hrs by cell titer based MTS assay
|
[PMID: 35476936] |
| MG-63 | IC50 |
0.55 μM
Compound: 6f
|
Antiproliferative activity against human MG-63 cells assessed as inhibition of cell growth incubated for 48 hrs by cell titer based MTS assay
Antiproliferative activity against human MG-63 cells assessed as inhibition of cell growth incubated for 48 hrs by cell titer based MTS assay
|
[PMID: 35476936] |
STAT3-IN-13 (compound 6f) (48 hours) has anti-proliferative activity with IC50 values of 0.25, 0.11 and 0.55 μM for 143B, HOS and MG63 cells, respectively[1].
STAT3-IN-13 (compound 6f) (0.001-100 μM) binds to STAT3 and interacts with STAT3586−685 in a concentration dependent manner with a KD of 0.96 μM[1].
STAT3-IN-13 (compound 6f) (0-1.0 μM; 24 hours; 143B and HOS cells) inhibits STAT3 Y705 phosphorylation and suppresses STAT3 in tumor cells[1].
STAT3-IN-13 (compound 6f) (0-1.0 μM; 48 hours; 143B cells) induces apoptosis in a dose-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:143B cells
-
Concentration:0, 0.2, 0.5, and 1 μM
-
Incubation Time:48 hours
-
Result:Increased the percentage of apoptosis rates of 3.4%, 8.7%, 10.7%, and 23.3% at 0, 0.2, 0.5 and 1 μM, respectively.
-
Cell Line:143B and HOS cells
-
Concentration:0, 0.2, 0.5 and 1.0 μM
-
Incubation Time:24 hours
-
Result:Inhibited STAT3 Y705 phosphorylation and downstream target gene expression including Bcl-2 and VEGF, retained good antitumor activities against control tumor cells without STAT3 knockdown.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Nude mice
-
Dosage:10 and 20 mg/kg
-
Administration:Intraperitoneal injection; twice daily, for 4 weeks
-
Result:Suppressed tumor weight at a dose of 10 mg/kg.
化学情報
-
CAS 番号 2248552-86-3
-
性状 Solid
-
分子量 436.49
-
分子式 C21H20N6O3S
-
Color Yellow to brown
-
SMILES
N#CC1=C(SC2=C1CCN(C2)C(NC3=CC=C(C=C3)OC)=O)NC(C(C=N4)=CN4C)=O
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Neoplasia
2025 Nov:69:101232. PMID: 40975978
溶剤 & 溶解度
DMSO : 125 mg/mL (286.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 6.25 mg/mL (14.32 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 6.25 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
-
データシート (273 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2910 mL | 11.4550 mL | 22.9100 mL | 57.2751 mL |
| 5 mM | 0.4582 mL | 2.2910 mL | 4.5820 mL | 11.4550 mL | |
| 10 mM | 0.2291 mL | 1.1455 mL | 2.2910 mL | 5.7275 mL | |
| 15 mM | 0.1527 mL | 0.7637 mL | 1.5273 mL | 3.8183 mL | |
| 20 mM | 0.1146 mL | 0.5728 mL | 1.1455 mL | 2.8638 mL | |
| 25 mM | 0.0916 mL | 0.4582 mL | 0.9164 mL | 2.2910 mL | |
| 30 mM | 0.0764 mL | 0.3818 mL | 0.7637 mL | 1.9092 mL | |
| 40 mM | 0.0573 mL | 0.2864 mL | 0.5728 mL | 1.4319 mL | |
| 50 mM | 0.0458 mL | 0.2291 mL | 0.4582 mL | 1.1455 mL | |
| 60 mM | 0.0382 mL | 0.1909 mL | 0.3818 mL | 0.9546 mL | |
| 80 mM | 0.0286 mL | 0.1432 mL | 0.2864 mL | 0.7159 mL | |
| 100 mM | 0.0229 mL | 0.1146 mL | 0.2291 mL | 0.5728 mL |
STAT3-IN-13 関連分類
HY-150603 関連分類
Powered by Bioz