3-Nitrocoumarin
Based on 4 publication(s) in Google Scholar
3-Nitrocoumarin is an inhibitor for phospholipase C that blocks the generation of inositol triphosphate (IP3), thereby affecting the calcium signaling pathway.
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- Purity: 100.0%
- CAS No.: 28448-04-6
- 화학식: C9H5NO4
- 분자량:191.14
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 3-Nitrocoumarin
MoreAll Phospholipase Isoforms
More
Biological Activity
3-Nitrocoumarin (50 mg/mL, 6 min) inhibits glucose-induced increase of intracellular calcium ions in yeast strain WPY260[2].
3-Nitrocoumarin inhibits PLC in Neurospora crassa with an IC50 of 10 µg/mL. 3-Nitrocoumarin also inhibits the growth of N. crassa, causes morphological changes of apical hyperbranching in hyphae[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 28448-04-6
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Appearance Solid
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분자량 191.14
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화학식 C9H5NO4
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Color Light yellow to yellow
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SMILES
O=C1C([N+]([O-])=O)=CC2=CC=CC=C2O1
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (4)
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Journal Impact Factor
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Most Recent
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Theranostics
FGF19/SOCE/NFATc2 signaling circuit facilitates the self-renewal of liver cancer stem cells. [Abstract]2021 Mar 5;11(10):5045-5060. PMID: 33754043 -
Cell Rep Med
2025 Sep 16;6(9):102324. PMID: 40897177 -
J Hazard Mater
PLCβ4 driven by cadmium-exposure during gestation and lactation contributes to cognitive deficits by suppressing PIP2/PLCγ1/CREB/BDNF signaling pathway in male offspring. [Abstract]2024 May 28:474:134756. PMID: 38820747 -
Blood Adv
PLCG2 Exon-Skipped Variants: Insights into Their Potential Role in Chronic Lymphocytic Leukemia. [Abstract]2026 Jun 5:bloodadvances.2025019077. PMID: 42263669
용액&용해도
DMSO : ≥ 100 mg/mL (523.18 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (276 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. Tisi R, et al., Phospholipase C is required for glucose-induced calcium influx in budding yeast. FEBS Lett. 2002 Jun 5;520(1-3):133-8. [Content Brief]
[2]. Silverman-Gavrila LB, et al., An IP3-activated Ca2+ channel regulates fungal tip growth. J Cell Sci. 2002 Dec 15;115(Pt 24):5013-25. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.2318 mL | 26.1588 mL | 52.3177 mL | 130.7942 mL |
| 5 mM | 1.0464 mL | 5.2318 mL | 10.4635 mL | 26.1588 mL | |
| 10 mM | 0.5232 mL | 2.6159 mL | 5.2318 mL | 13.0794 mL | |
| 15 mM | 0.3488 mL | 1.7439 mL | 3.4878 mL | 8.7196 mL | |
| 20 mM | 0.2616 mL | 1.3079 mL | 2.6159 mL | 6.5397 mL | |
| 25 mM | 0.2093 mL | 1.0464 mL | 2.0927 mL | 5.2318 mL | |
| 30 mM | 0.1744 mL | 0.8720 mL | 1.7439 mL | 4.3598 mL | |
| 40 mM | 0.1308 mL | 0.6540 mL | 1.3079 mL | 3.2699 mL | |
| 50 mM | 0.1046 mL | 0.5232 mL | 1.0464 mL | 2.6159 mL | |
| 60 mM | 0.0872 mL | 0.4360 mL | 0.8720 mL | 2.1799 mL | |
| 80 mM | 0.0654 mL | 0.3270 mL | 0.6540 mL | 1.6349 mL | |
| 100 mM | 0.0523 mL | 0.2616 mL | 0.5232 mL | 1.3079 mL |
3-Nitrocoumarin Related Classifications
HY-111919 Related Classifications
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