BM 957

Two cancer cell lines (H1417 and H146 cell lines) are used. Induction of cell death by compounds (e.g., BM 957) in the H146 cell line. Cells are treated with different concentrations of the compounds (e.g., BM 957: 10, 30, 100 nM) for 24 hr. Cell viability is determined using a trypan blue exclusion assay^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Mice^[1]
Induction of cleavage of PARP and caspase-3 in H146 xenograft tumors by compounds 28 and BM 957. SCID mice bearing H146 xenograft tumors (100-200 mm³) are treated with vehicle control, single dose of 28 (50 mg/kg, i.v.) or BM 957 (25 mg/kg, i.v.). SCID mice bearing xenograft tumors (100 mm³) are treated with vehicle control, compound 28 at 50 mg/kg, i.v. or BM 957 at 25 mg/kg, i.v., daily, 5 days a week for two weeks. The tumor growth inhibition for both compounds is statistically highly significant with p=0.0033 for compound 28 versus vehicle control and p=0.0006 for BM 957 versus vehicle control, respectively, when the mean tumor volume reaches 750 mm³ in the vehicle treated group in both experiments^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.