Naspm
Based on 8 publication(s) in Google Scholar
Naspm (1-Naphthyl acetyl spermine), a synthetic analogue of Joro spider toxin, is a calcium permeable AMPA (CP-AMPA) receptors antagonist.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 95.15%
- CAS No.: 122306-11-0
- 화학식: C22H34N4O
- 분자량:370.53
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보관:Pure form -20°C, 3 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) Naspm
More- J Headache Pain. 2022 Aug 10;23(1):98. [Abstract]
- Cell Rep. 2023 Dec 3;42(12):113551. [Abstract]
- Cell Rep. 2020 Nov 10;33(6):108369. [Abstract]
- Neurobiol Dis. 2024 May:194:106471. [Abstract]
- iScience. 2023 Mar 3;26(4):106322. [Abstract]
- Neuroscience. 2021 Nov 1:475:220-228. [Abstract]
- Brain Res. 2026 Jul 15:1883:150291. [Abstract]
- Toxicol In Vitro. 2017 Oct:44:57-65. [Abstract]
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IF
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Oocyte | IC50 |
0.69 μM
Compound: 2
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Antagonist activity for [Ca2+] permeable Ionotropic glutamate receptor AMPA expressed in Xenopus oocytes by injection of rat brain mRNA
Antagonist activity for [Ca2+] permeable Ionotropic glutamate receptor AMPA expressed in Xenopus oocytes by injection of rat brain mRNA
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[PMID: 11392532] |
| Oocyte | IC50 |
0.69 μM
Compound: 2-NAS
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Antagonistic activity against Ca++permeable Ionotropic glutamate receptor AMPA as concentration of compound that reduces the inward current induced by kainate by 50% in Xenopus oocytes
Antagonistic activity against Ca++permeable Ionotropic glutamate receptor AMPA as concentration of compound that reduces the inward current induced by kainate by 50% in Xenopus oocytes
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[PMID: 11551773] |
NASPM selectively suppresses the inwardly rectifying and Ca2+-permeable AMPA receptors expressed in type II neurons. It has no effect on AMPA receptors in type I neurons. At -60 mV, NASPM suppresses AMPA receptors in type II neurons with an IC50 value of 0.33 μM. The blocking effect of NASPM on the Ca2+-permeable AMPA receptors is use and voltage-dependent[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 122306-11-0
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Appearance Liquid (Density: 1.062 g/cm3)
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분자량 370.53
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화학식 C22H34N4O
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Color Colorless to light yellow
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SMILES
O=C(NCCCNCCCCNCCCN)CC1=C2C=CC=CC2=CC=C1
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Synonyms
1-Naphthylacetyl spermine
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Pure form -20°C 3 years In solvent -80°C 2 years -20°C 1 year
Publications (8)
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Journal Impact Factor
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Most Recent
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J Headache Pain
Dopamine receptor D2 regulates GLUA1-containing AMPA receptor trafficking and central sensitization through the PI3K signaling pathway in a male rat model of chronic migraine. [Abstract]2022 Aug 10;23(1):98. PMID: 35948867 -
Cell Rep
Single-cell RNA sequencing uncovers the cell type-dependent transcriptomic changes in the retrosplenial cortex after peripheral nerve injury. [Abstract]2023 Dec 3;42(12):113551. PMID: 38048224 -
Cell Rep
Restoration of Cingulate Long-Term Depression by Enhancing Non-apoptotic Caspase 3 Alleviates Peripheral Pain Hypersensitivity. [Abstract]2020 Nov 10;33(6):108369. PMID: 33176141 -
Neurobiol Dis
Spinal nerve transection-induced upregulation of SAP97 via promoting membrane trafficking of GluA1-containing AMPA receptors in the dorsal horn contributes to the pathogenesis of neuropathic pain. [Abstract]2024 May:194:106471. PMID: 38461868 -
iScience
Synaptic scaling of corticostriatal circuits underlies hyperactivity in GABA Transporter-1 deficient mice. [Abstract]2023 Mar 3;26(4):106322. PMID: 36968092 -
Neuroscience
2021 Nov 1:475:220-228. PMID: 34509547 -
Brain Res
Upregulation of synapse-associated protein 97 in the spinal dorsal horn exacerbates inflammatory pain through mediating GluA1-containing AMPARs membrane trafficking. [Abstract]2026 Jul 15:1883:150291. PMID: 41916464 -
Toxicol In Vitro
Propofol inhibits invasion and proliferation of C6 glioma cells by regulating the Ca2+ permeable AMPA receptor-system xc- pathway. [Abstract]2017 Oct:44:57-65. PMID: 28663055
Naspm purchased from MedChemExpress. Usage Cited in: Toxicol In Vitro. 2017 Oct:44:57-65. [Abstract]
A Transwell invasion assay of cells is used to measure invasiveness after treatment with L-glutamate, propofol, 1-Naphthyl acetyl spermine (NAS), or (R, S)-AMPA.
용액&용해도
DMSO : 100 mg/mL (269.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (6.75 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (6.75 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (268 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6988 mL | 13.4942 mL | 26.9884 mL | 67.4709 mL |
| 5 mM | 0.5398 mL | 2.6988 mL | 5.3977 mL | 13.4942 mL | |
| 10 mM | 0.2699 mL | 1.3494 mL | 2.6988 mL | 6.7471 mL | |
| 15 mM | 0.1799 mL | 0.8996 mL | 1.7992 mL | 4.4981 mL | |
| 20 mM | 0.1349 mL | 0.6747 mL | 1.3494 mL | 3.3735 mL | |
| 25 mM | 0.1080 mL | 0.5398 mL | 1.0795 mL | 2.6988 mL | |
| 30 mM | 0.0900 mL | 0.4498 mL | 0.8996 mL | 2.2490 mL | |
| 40 mM | 0.0675 mL | 0.3374 mL | 0.6747 mL | 1.6868 mL | |
| 50 mM | 0.0540 mL | 0.2699 mL | 0.5398 mL | 1.3494 mL | |
| 60 mM | 0.0450 mL | 0.2249 mL | 0.4498 mL | 1.1245 mL | |
| 80 mM | 0.0337 mL | 0.1687 mL | 0.3374 mL | 0.8434 mL | |
| 100 mM | 0.0270 mL | 0.1349 mL | 0.2699 mL | 0.6747 mL |
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