Obacunone
Based on 4 publication(s) in Google Scholar
Obacunone is a highly oxidized triterpenoid limonoid isolated from citrus plants. Obacunone exerts its anticancer effects by inducing apoptosis. Obacunone also protects retinal pigment epithelial (RPE) cells from ultraviolet (UV) radiation (UVR)-induced oxidative damage.
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- Purity: 99.75%
- CAS No.: 751-03-1
- 화학식: C26H30O7
- 분자량:454.51
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보관:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) Obacunone
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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IF
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WB
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RT-PCR
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Histological Imaging/Staining
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| J774.A1 | IC50 |
83.61 μM
Compound: 5
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Antiinflammatory activity in mouse J774A1 cells assessed as reduction of LPS-induced nitric oxide production treated 2 hrs before LPS addition measured after 20 hrs by using Griess reagent
Antiinflammatory activity in mouse J774A1 cells assessed as reduction of LPS-induced nitric oxide production treated 2 hrs before LPS addition measured after 20 hrs by using Griess reagent
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[PMID: 23688954] |
| MCF7 | IC50 |
0.02 μM
Compound: 11g
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Antiproliferative activity against human MCF7 cells incubated for 48 hrs by [3H]-thymidine incorporation assay
Antiproliferative activity against human MCF7 cells incubated for 48 hrs by [3H]-thymidine incorporation assay
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[PMID: 33289552] |
| MDA-MB-435 | IC50 |
6.8 μM
Compound: 11g
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Antiproliferative activity against human MDA-MB-435 cells incubated for 48 hrs by [3H]-thymidine incorporation assay
Antiproliferative activity against human MDA-MB-435 cells incubated for 48 hrs by [3H]-thymidine incorporation assay
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[PMID: 33289552] |
| SW480 | IC50 |
56 μM
Compound: 11g
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Antiproliferative activity against human SW480 cells incubated for 72 hrs by MTT assay
Antiproliferative activity against human SW480 cells incubated for 72 hrs by MTT assay
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[PMID: 33289552] |
Obacunone (1-50 μM; 48 h) effectively inhibits UVR-induced cytotoxicity[2].
Obacunone (25 μM; 1 h+12 h) improves UVR-induced oxidative damage in ARPE-19 cells and inhibits reactive oxygen species accumulation, mitochondrial depolarization, lipid peroxidation, and single-stranded DNA accumulation in human ARPE-19 cells[2].
Obacunone (25 μM; 1 h+12 h) slows UVR-induced apoptosis in human ARPE-19 cells and murine RPE cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 751-03-1
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Appearance Solid
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분자량 454.51
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화학식 C26H30O7
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Color White to off-white
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SMILES
O=C(OC1(C)C)C=C[C@]([C@@]1([H])C2)(C)[C@@]3([H])CC[C@]4(C)[C@@]([C@H]5C(O[C@H]4C6=COC=C6)=O)(O5)[C@]3(C)C2=O
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Structure Classification
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (4)
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Journal Impact Factor
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Most Recent
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Int Immunopharmacol
Obacunone inhibits RIPK1/RIPK3/MLKL-mediated necroptosis by suppressing mitochondrial ROS and MIF signaling in intestinal ischemia-reperfusion injury. [Abstract]2026 Jan 1;168(Pt 2):115901. PMID: 41317539 -
ACS Omega
Identification of New Modulators and Inhibitors of Palmitoyl-Protein Thioesterase 1 for CLN1 Batten Disease and Cancer. [Abstract]2024 Feb 28;9(10):11870-11882. PMID: 38496939 -
Mol Med Rep
Obacunone inhibits ferroptosis through regulation of Nrf2 homeostasis to treat diabetic nephropathy. [Abstract]2025 May;31(5):135. PMID: 40116089
Obacunone purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 May;31(5):135. [Abstract]
CCK-8 assay was used to measure the viability of HK-2 cells treatment with Obacunone (OB) solutions at different concentrations (0, 5, 10, 20, 40, 80 and 160 µM).
Obacunone purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 May;31(5):135. [Abstract]
Levels of ROS in HK-2 cells from each group were measured using immunofluorescence treated with Obacunone (OB) (20, 40, 80 μM).
Obacunone purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 May;31(5):135. [Abstract]
Protein levels of GPX4, ACSL4, SLC7A11 and FTH1 in HK-2 cells from each group were measured using western blotting treated with Obacunone (OB) (20, 40, 80 μM).
Obacunone purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 May;31(5):135. [Abstract]
The expression levels of Nrf2 and KEAP1 in HK-2 cells from the Control, HG, HG + Obacunone (OB) 20 µM, HG + OB 40 µM and HG + OB 80 µM groups were assessed using reverse transcription-quantitative PCR.
Obacunone purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 May;31(5):135. [Abstract]
Severity of renal pathological damage in each group of rats was observed using hematoxylin and eosin staining treated with Obacunone (OB) (25, 50, 100 mg/kg, p.o.).
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J Orthop Surg Res
Obacunone acts as a histone deacetylase 1 inhibitor to limit p38MAPK signaling and alleviate osteoarthritis progression. [Abstract]2025 May 3;20(1):441. PMID: 40319261
용액&용해도
DMSO : 100 mg/mL (220.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.50 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.50 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Chidambara Murthy KN, et al. Obacunone and obacunone glucoside inhibit human colon cancer (SW480) cells by the induction of apoptosis. Food Chem Toxicol. 2011 Jul;49(7):1616-25. [Content Brief]
[2]. Huang DR, et al. Obacunone protects retinal pigment epithelium cells from ultra-violet radiation-induced oxidative injury. Aging (Albany NY). 2021 Feb 1;13(8):11010-11025. [Content Brief]
[3]. Xu S, et al. Obacunone activates the Nrf2-dependent antioxidant responses. Protein Cell. 2016 Sep;7(9):684-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.2002 mL | 11.0009 mL | 22.0017 mL | 55.0043 mL |
| 5 mM | 0.4400 mL | 2.2002 mL | 4.4003 mL | 11.0009 mL | |
| 10 mM | 0.2200 mL | 1.1001 mL | 2.2002 mL | 5.5004 mL | |
| 15 mM | 0.1467 mL | 0.7334 mL | 1.4668 mL | 3.6670 mL | |
| 20 mM | 0.1100 mL | 0.5500 mL | 1.1001 mL | 2.7502 mL | |
| 25 mM | 0.0880 mL | 0.4400 mL | 0.8801 mL | 2.2002 mL | |
| 30 mM | 0.0733 mL | 0.3667 mL | 0.7334 mL | 1.8335 mL | |
| 40 mM | 0.0550 mL | 0.2750 mL | 0.5500 mL | 1.3751 mL | |
| 50 mM | 0.0440 mL | 0.2200 mL | 0.4400 mL | 1.1001 mL | |
| 60 mM | 0.0367 mL | 0.1833 mL | 0.3667 mL | 0.9167 mL | |
| 80 mM | 0.0275 mL | 0.1375 mL | 0.2750 mL | 0.6876 mL | |
| 100 mM | 0.0220 mL | 0.1100 mL | 0.2200 mL | 0.5500 mL |
Obacunone Related Classifications
HY-N0428 Related Classifications
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