USP30-IN-1
Based on 1 Customer Validation
USP30-IN-1 is a potent and selective USP30 inhibitor, with IC50 values of 94 nM and 4 nM in vivo and in vitro, respectively, and Ki values of 0.33 μM (Krippendorf method) and 0.38 μM (traditional method), respectively. USP30-IN-1 covalently binds to the catalytic cysteine (Cys77) of USP30, blocks ubiquitin substrate binding to inhibit deubiquitination, and ultimately induces mitophagy. USP30-IN-1 can be used for the research of idiopathic Parkinson's disease and mitophagy-related diseases.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 99.41%
- CAS No.: 2511541-78-7
- 화학식: C17H15N5O2
- 분자량:321.33
-
보관:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
용액&용해도
- 순도&문서
- References
-
Help & FAQs
Biological Activity
|
USP30 94 nM (IC50, in vivo) |
USP30 4 nM (IC50, in vitro) |
USP30-IN-1 (compound USP30-I-1) (0.01-10 μM; 1 h) blocks the binding of the HA-Ub-PA probe to endogenous USP30 in lysates of SH-SY5Y neuroblastoma cells, potently and specifically inhibits USP30 activity, but weakly inhibits USP10 activity[1].
USP30-IN-1 (30 min) dose-dependently inhibits the activity of USP30 in catalyzing the cleavage of ubiquitin-rhodamine substrate in a cell-free system[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:SH-SY5Y neuroblastoma cell lysates
-
Concentration:0, 0.01, 0.1, 1, 10 μM
-
Incubation Time:1 h
-
Result:Dose-dependently inhibited the labeling of endogenous USP30 by HA-Ub-PA, demonstrating highly selective and potent target inhibition.
Chemical Information
-
CAS No. 2511541-78-7
-
Appearance Solid
-
분자량 321.33
-
화학식 C17H15N5O2
-
SMILES
N#CC1=CC=CC(C2=CN=C(C(N[C@@H]3C[C@@H](C)N(C#N)C3)=O)O2)=C1
-
선적
Room temperature in continental US; may vary elsewhere.
-
보관
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
용액&용해도
DMSO : 100 mg/mL (311.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1121 mL | 15.5603 mL | 31.1207 mL | 77.8016 mL |
| 5 mM | 0.6224 mL | 3.1121 mL | 6.2241 mL | 15.5603 mL | |
| 10 mM | 0.3112 mL | 1.5560 mL | 3.1121 mL | 7.7802 mL | |
| 15 mM | 0.2075 mL | 1.0374 mL | 2.0747 mL | 5.1868 mL | |
| 20 mM | 0.1556 mL | 0.7780 mL | 1.5560 mL | 3.8901 mL | |
| 25 mM | 0.1245 mL | 0.6224 mL | 1.2448 mL | 3.1121 mL | |
| 30 mM | 0.1037 mL | 0.5187 mL | 1.0374 mL | 2.5934 mL | |
| 40 mM | 0.0778 mL | 0.3890 mL | 0.7780 mL | 1.9450 mL | |
| 50 mM | 0.0622 mL | 0.3112 mL | 0.6224 mL | 1.5560 mL | |
| 60 mM | 0.0519 mL | 0.2593 mL | 0.5187 mL | 1.2967 mL | |
| 80 mM | 0.0389 mL | 0.1945 mL | 0.3890 mL | 0.9725 mL | |
| 100 mM | 0.0311 mL | 0.1556 mL | 0.3112 mL | 0.7780 mL |
USP30-IN-1 Related Classifications
HY-161909 Related Classifications
Powered by Bioz