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Cat. No.: HY-170983

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MC-25B

MC-25B is a DCAF16-recruiting FKBP12 PROTAC degrader with a DC₅₀ of 0.35 μM. MC-25B promotes the formation of a ternary complex with DCAF16 and nuclear-localized FKBP12_NLS, thereby mediating DCAF16-dependent degradation of FKBP12_NLS via the proteasome and Cullin-RING ligase pathway.
(Pink: FKBP12 ligand (HY-170988); Blue: DCAF16 ligand (HY-170986); Black: linker (HY-128844)).

For research use only. We do not sell to patients.

Formula: C₆₅H₉₂ClN₇O₁₄
Molecular Weight:1230.92

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (268 KB)

MC-25B Chemical Structure

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Bioz

Biological Activity

Description

MC-25B is a DCAF16-recruiting FKBP12 PROTAC degrader with a DC₅₀ of 0.35 μM. MC-25B promotes the formation of a ternary complex with DCAF16 and nuclear-localized FKBP12_NLS, thereby mediating DCAF16-dependent degradation of FKBP12_NLS via the proteasome and Cullin-RING ligase pathway^[1]. (Pink: FKBP12 ligand (HY-170988); Blue: DCAF16 ligand (HY-170986); Black: linker (HY-128844)).

IC₅₀ & Target^[1]

FKBP12

0.35 μM (DC₅₀)

In Vitro

MC-25B (1 μM; 24 h) induces selective, DCAF16-dependent degradation of FKBP12 in HEK293T cells expressing FLAG-FKBP12_NLS, with only one identified DCAF16-independent off-target effect^[1].
MC-25B (0.1-5 μM; 8 h) induces dose-dependent degradation of FLAG-FKBP12_NLS in HEK293T cells overexpressing HA-DCAF16, with a DC₅₀ of 0.35 μM and a Dmax of 89%^[1].
MC-25B (1-2 μM; 8 h) induces the degradation of FLAG-FKBP12_NLS in HEK293T cells expressing the DCAF16_3Cys mutant (retaining only C177−179), confirming that this cysteine cluster plays a critical role in MC-25B-mediated degradation^[1].
MC-25B (incubated for 72 h) inhibits the viability of HEK293T cells, with an IC₅₀ value of 2.2 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

HEK293T cells overexpressing HA-DCAF16 and FLAG-FKBP12_NLS
Concentration:

0.1 μM, 0.2 μM, 0.5 μM, 1 μM, 2 μM, 5 μM
Incubation Time:

8 h
Result:

Induced dose-dependent degradation of FLAG-FKBP12_NLS, with a maximum degradation (D_max) of 89% and a half-maximal degradation concentration (DC₅₀) of 0.35 μM.

Chemical Information

Molecular Weight 1230.92
Formula C₆₅H₉₂ClN₇O₁₄
SMILES

O=C(CN1CCN(C2=CC=CC(C(N3CCC(N(C4CC4)C(CCl)=O)CC3)=O)=C2)CC1)NCCCOCCOCCOCCCNC(COC5=CC=CC([C@H](OC([C@@H]6CCCCN6C(C(C(C)(C)CC)=O)=O)=O)CCC7=CC(OC)=C(OC)C=C7)=C5)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (268 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Campos MA, et al. Discovery of DCAF16 Binders for Targeted Protein Degradation. ACS chemical biology. 2025 Feb 21;20(2):479-488. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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