Muramyl dipeptide (Synonyms: MDP)

Name: Muramyl dipeptide
Brand: MedChemExpress
SKU: HY-127090
Rating: 5.0 (10 reviews)

Cat. No.: HY-127090 Purity: 99.07%: Data Sheet
COA

SDS
Handling Instructions
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Technical Support

Muramyl dipeptide (MDP) is a synthetic immunoreactive peptide, consisting of N-acetyl muramic acid attached to a short amino acid chain of L-Ala-D-isoGln. Muramyl dipeptide is an inducer of bone formation through induction of Runx2. Muramyl dipeptide directly enhances osteoblast differentiation by up-regulating Runx2 gene expression through MAPK pathways. Muramyl dipeptide is a NLRP1 agonist.

For research use only. We do not sell to patients.

CAS No. : 53678-77-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of Muramyl dipeptide:

Muramyl dipeptide-d₃ Get quote

10 Publications Citing Use of MCE Muramyl dipeptide

ELISA

: Muramyl dipeptide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2025 Aug 30:354:120514. [Abstract]; Western blot analysis of p-Smad3, Runx2, OPN, and OCN protein levels and quantification. Runx2 agonist Muramyl dipeptide (MDP) (10 μg/mL; 24 h) partially restored osteogenic marker expression in Smad2/3-overexpressed MC3T3-E1 cells.

: Muramyl dipeptide purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 16;143(Pt 3):113622. [Abstract]; Muramyl dipeptide (MDP) (10 μg/ml, 1 h) enhanced the colocalization of VCPIP1 and Erbin in RAW264.7 cells.

: Muramyl dipeptide purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 16;143(Pt 3):113622. [Abstract]; Muramyl dipeptide (MDP) (10 μg/ml, 1 h) treatment increased the protein expression of VCPIP1, Erbin, and the phosphorylation levels of IKKα/β, IκBα, and NF-κB in RAW264.7 cells.

: Muramyl dipeptide purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 16;143(Pt 3):113622. [Abstract]; Muramyl dipeptide (MDP) (10 μg/mL, 6 h) significantly increased levels of inflammatory factors TNF-α, IL-6, and IL-1β in the cultured supernatant of RAW264.7 cells.

: Muramyl dipeptide purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 16;143(Pt 3):113622. [Abstract]; Muramyl dipeptide (MDP) (10 μg/ml, 1 h) induced increased nuclear p65 in both BMDMs from VCPIP1 KO and WT mice.

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NLRP3 NLRP1 NOD1 NOD2

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]^[2]

p38 MAPK

NLRP1

In Vitro

Muramyl dipeptide (0.1-10 μg/mL; 24 hours) increases protein expression of Runx2 in a dose-dependent manner^[1].
Muramyl dipeptide (0.1-10 μg/mL; 6 hours) increases mRNA levels of Runx2 in a dose-dependent manner^[1].
Muramyl dipeptide indirectly attenuates osteoclast differentiation through a decreased RANKL/OPG ratio^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	MC3T3-E1 cells
Concentration:	0.1, 1, 10 µg/mL
Incubation Time:	24 hours
Result:	Increased protein expression of Runx2 in a dose-dependent manner.

RT-PCR^[1]

Cell Line:	MC3T3-E1 cells
Concentration:	0.1, 1, 10 µg/mL
Incubation Time:	6 hours
Result:	Increases mRNA levels of Runx2 in a dose-dependent manner.

In Vivo

Muramyl dipeptide (1.25 mg/kg; i.p.; twice) alleviates bone loss induced by osteoporosis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	RANKL-induced osteoporosis model (Five-week-old C57BL/6 mice)^[1]
Dosage:	1.25 mg/kg
Administration:	i.p.; twice (RANKL-induced osteoporosis for 3 weeks and euthanized at 4 weeks)
Result:	Significantly enhanced the trabecular bone volume (BV/TV) and trabecular number (Tb.N).

Clinical Trial

Molecular Weight

492.48

Formula

C₁₉H₃₂N₄O₁₁

CAS No.

53678-77-6

Appearance

Solid

Color

White to off-white

SMILES

C[C@@H](C(N[C@@H](C(N)=O)CCC(O)=O)=O)NC([C@H](O[C@H]1[C@@H]([C@H](OC([C@@H]1NC(C)=O)O)CO)O)C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (203.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (101.53 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0305 mL	10.1527 mL	20.3054 mL
5 mM	0.4061 mL	2.0305 mL	4.0611 mL
10 mM	0.2031 mL	1.0153 mL	2.0305 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 5 mg/mL (10.15 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 5 mg/mL (10.15 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 5 mg/mL (10.15 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.07%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (247 KB) HNMR (149 KB) RP-HPLC (218 KB) MS (150 KB)

Handling Instructions (2659 KB)

References

[1]. Park OJ, et al. Muramyl Dipeptide, a Shared Structural Motif of Peptidoglycans, Is a Novel Inducer of Bone Formation through Induction of Runx2. J Bone Miner Res. 2017 Jul;32(7):1455-1468. [Content Brief]

[2]. V Kaushal, et al. Neuronal NLRP1 inflammasome activation of Caspase-1 coordinately regulates inflammatory interleukin-1-beta production and axonal degeneration-associated Caspase-6 activation. Cell Death Differ. 2015 Oct;22(10):1676-86. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.0305 mL	10.1527 mL	20.3054 mL	50.7635 mL
	5 mM	0.4061 mL	2.0305 mL	4.0611 mL	10.1527 mL
	10 mM	0.2031 mL	1.0153 mL	2.0305 mL	5.0763 mL
	15 mM	0.1354 mL	0.6768 mL	1.3537 mL	3.3842 mL
	20 mM	0.1015 mL	0.5076 mL	1.0153 mL	2.5382 mL
	25 mM	0.0812 mL	0.4061 mL	0.8122 mL	2.0305 mL
	30 mM	0.0677 mL	0.3384 mL	0.6768 mL	1.6921 mL
	40 mM	0.0508 mL	0.2538 mL	0.5076 mL	1.2691 mL
	50 mM	0.0406 mL	0.2031 mL	0.4061 mL	1.0153 mL
	60 mM	0.0338 mL	0.1692 mL	0.3384 mL	0.8461 mL
	80 mM	0.0254 mL	0.1269 mL	0.2538 mL	0.6345 mL
	100 mM	0.0203 mL	0.1015 mL	0.2031 mL	0.5076 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Muramyl dipeptide Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Muramyl dipeptide53678-77-6MDPp38 MAPKNOD-like Receptor (NLR)peptidoglycansboneformationNod2osteoblastosteoclastdifferentiationRANKL/OPGratioinductionInhibitorinhibitorinhibit

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Muramyl dipeptide (Synonyms: MDP)

Customer Review

Other Forms of Muramyl dipeptide:

Muramyl dipeptide Related Antibodies

10 Publications Citing Use of MCE Muramyl dipeptide

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All p38 MAPK Isoform Specific Products:

View All NOD-like Receptor (NLR) Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Muramyl dipeptide Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Muramyl dipeptide Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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