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Cat. No.: HY-187984

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Natrin

Natrin is a cysteine-rich secretory protein (CRISP) toxin found in snake venom that acts as an ion channel modulator. Natrin blocks the large-conductance calcium-activated potassium channel BK_Ca with an IC₅₀ of 34.4 nM; it acts as a non-competitive antagonist to inhibit the ryanodine binding capacity and calcium release channel activity of RyR1, with an IC₅₀ of 1.03 μM for purified RyR1. Natrin induces vasoconstrictor responses without affecting basal tension. Natrin induces apoptosis in human hepatoma cells, elevating the Bax/Bcl‑2 ratio through the mitochondrial apoptotic pathway and causing metabolic perturbation. Natrin can be used in research on diseases such as hepatocellular carcinoma.

For research use only. We do not sell to patients.

CAS No.: 69633-04-1
Formula: C₈H₇Cl₃O₅S
Molecular Weight:321.56

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Natrin Chemical Structure

CAS No.: 69633-04-1

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
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Classifications
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Biological Activity

Description

IC₅₀ & Target

^[4]^[5]

Bax

Bcl-2

Kv1.3

All Calcium Channel Isoforms :

All Bcl-2 Family Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
SMMC-7721	IC50	49.33 mg/L	Cytotoxicity against human SMMC-7721 hepatocellular carcinoma cells assessed as viability reduction incubated 24 hrs by MTT assay. Cytotoxicity against human SMMC-7721 hepatocellular carcinoma cells assessed as viability reduction incubated 24 hrs by MTT assay.	30508545

In Vitro

Anti-cancerApoptosisLipid MetabolismLocal Vascular RegulationOthers

Anti-cancer

Natrin (25-200 mg/L; 24 h) inhibits SMMC-7721 cell viability with an IC₅₀ of 49.33 mg/L^[4].

Anti-cancer

Apoptosis

Natrin (25-50 mg/L; 24 h) induces apoptotic morphological changes in SMMC-7721 cells^[4].
Natrin induces apoptosis in SMMC-7721 cells by reducing Bcl-2 expression and increasing the Bax/Bcl-2 ratio through the mitochondrial pathway^[4].

Anti-cancer

Lipid Metabolism

Natrin (50 mg/L; 24 h) perturbs the metabolome of SMMC-7721, with 11 potential biomarkers and sphingolipid metabolism being most significantly affected^[4].

Local Vascular Regulation

Natrin (1 μM) does not change basal tension but enhances high K⁺-induced contraction of endothelium-denuded mouse thoracic aortic rings by approximately 23%^[1].
Natrin blocks BK_Ca currents in isolated mouse vascular smooth muscle cells, with an IC₅₀ of 34.4 nM and a Hill coefficient of 1.02^[1].

Others

Natrin specifically binds RyR1 in rabbit skeletal muscle SR vesicles in co-immunoprecipitation experiments and reduces RyR1-mediated calcium efflux^[3].
Natrin inhibits the binding of [³H]ryanodine to rabbit skeletal muscle SR vesicles with an IC₅₀ of 3.1 µM and decreases B_max at 3 µM; it inhibits the binding of [³H]ryanodine to purified RyR1 with an IC₅₀ of 1.03 µM and decreases B_max at 1 µM^[3].
Natrin induces contractile responses in endothelium-denuded mouse thoracic aorta and blocks high-conductance calcium-activated potassium channels, the skeletal muscle isoform of ryanodine receptors, and the voltage-gated potassium channel K_v1.3^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:

human SMMC-7721 hepatocellular carcinoma cells
Concentration:

25, 50, 75, 100, 150, 200 mg/L
Incubation Time:

24 h
Result:

Inhibited SMMC-7721 proliferation in a concentration-dependent manner.
IC₅₀ = 49.33 mg/L.

Apoptosis Analysis^[4]

Cell Line:

human SMMC-7721 hepatocellular carcinoma cells
Concentration:

25, 50 mg/L
Incubation Time:

24 h
Result:

Induced apoptotic morphological changes in SMMC-7721 cells.

Related Protocols :

Chemical Information

CAS No. 69633-04-1
Molecular Weight 321.56
Formula C₈H₇Cl₃O₅S
SMILES

O=S(O)(OCCOC1=CC(Cl)=C(C=C1Cl)Cl)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Wang J, et al. Blocking effect and crystal structure of natrin toxin, a cysteine-rich secretory protein from Naja atra venom that targets the BKCa channel. Biochemistry. 2005 Aug 2;44(30):10145-52.

[2]. Deka A, et al. Naja kaouthia venom protein, Nk-CRISP, upregulates inflammatory gene expression in human macrophages. International journal of biological macromolecules. 2020 Oct 1;160:602-11.

[3]. Zhou Q, et al. Structural and functional characterization of ryanodine receptor-natrin toxin interaction. Biophysical journal. 2008 Nov 01;95(9):4289-99.

[4]. Lu S, et al. Metabolomic study of natrin-induced apoptosis in SMMC-7721 hepatocellular carcinoma cells by ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry. International journal of biological macromolecules. 2019 Mar 1;124:1264-73.

[5]. Averin AS, et al. Cardiovascular Effects of Snake Toxins: Cardiotoxicity and Cardioprotection. Acta naturae. 2021;13(3):4-14.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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Natrin

All Calcium Channel Isoforms

All Bcl-2 Family Isoforms

Biological Activity

Description

IC50 & Target

Cellular Effect

In Vitro

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

Natrin Related Classifications

HY-187984 Related Classifications

Bioz

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