NCGC00685960
Based on 1 Customer Validation
NCGC00685960 is a Nicotinamide N-methyltransferase (NNMT) inhibitor with an IC50 <10 nM. NCGC00685960 has potent antitumor activity. NCGC00685960 increases H3K27 trimethylation levels in ovarian cancer cells and inhibits α-SMA expression in NNMT-expressing ovarian fibroblasts. NCGC00685960 reduces 1-MNA levels, reverses SAM and H3K27 hypomethylation and significantly impairs collagen contractility in cancer-associated fibroblasts (CAFs). NCGC00685960 can be used for cancers research, such as ovarian cancer, breast cancer, colon cancer.
For research use only. We do not sell to patients.
- Purity: 99.97%
- CAS No.: 3091000-99-3
- Formula: C24H32ClN5O2
- Molecular Weight:458.00
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
NCGC00685960 (NNMTi) (3-72 h) inhibits NNMT activity, reduces 1-methylnicotinamide (1-MNA) production, restores SAM levels, reverses H3K27me3 hypomethylation, downregulates complement factor (e.g., C3) secretion, decreases the expression of CAF markers (α-SMA, Ly6C, MHCII) and collagen contractility, reduces the recruitment of immunosuppressive Ly6C+PD-L1+ monocytes, and restores CD8+ T cell activation and proliferation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6NCrl with ovarian cancer, breast cancer and colon cancer[1]
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Dosage:4, 25 mg/kg
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Administration:I.p. or i.t.; daily; 42 days
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Result:Reduced primary tumour weight.
Decreased C3 protein levels in tumour tissue compared to vehicle control.
Reduced number of immunosuppressive Ly6C+PD-L1+ monocytes per mg tumour compared to vehicle control.
Increased CD8+ granzyme B+ T cells per mg tumour compared to vehicle control.
Chemical Information
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CAS No. 3091000-99-3
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Appearance Solid
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Molecular Weight 458.00
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Formula C24H32ClN5O2
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Color White to off-white
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SMILES
O=C1NC2=C(C=CC=C2N3N=C([C@@H]4CN(CCC5CCCCC5)CCC4)NC3=O)C=C1.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
In Vitro:
DMSO : ≥ 200 mg/mL (436.68 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (10.92 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (10.92 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1834 mL | 10.9170 mL | 21.8341 mL | 54.5852 mL |
| 5 mM | 0.4367 mL | 2.1834 mL | 4.3668 mL | 10.9170 mL | |
| 10 mM | 0.2183 mL | 1.0917 mL | 2.1834 mL | 5.4585 mL | |
| 15 mM | 0.1456 mL | 0.7278 mL | 1.4556 mL | 3.6390 mL | |
| 20 mM | 0.1092 mL | 0.5459 mL | 1.0917 mL | 2.7293 mL | |
| 25 mM | 0.0873 mL | 0.4367 mL | 0.8734 mL | 2.1834 mL | |
| 30 mM | 0.0728 mL | 0.3639 mL | 0.7278 mL | 1.8195 mL | |
| 40 mM | 0.0546 mL | 0.2729 mL | 0.5459 mL | 1.3646 mL | |
| 50 mM | 0.0437 mL | 0.2183 mL | 0.4367 mL | 1.0917 mL | |
| 60 mM | 0.0364 mL | 0.1820 mL | 0.3639 mL | 0.9098 mL | |
| 80 mM | 0.0273 mL | 0.1365 mL | 0.2729 mL | 0.6823 mL | |
| 100 mM | 0.0218 mL | 0.1092 mL | 0.2183 mL | 0.5459 mL |
NCGC00685960 Related Classifications
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