Oudemansin A
Oudemansin A is an antibiotic. Oudemansin A acts on fungi to inhibit protein, RNA and DNA synthesis.
For research use only. We do not sell to patients.
- CAS No.: 73341-71-6
- Formula: C17H22O4
- Molecular Weight:290.35
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| KB | IC50 |
5.48 μg/mL
Compound: (-)-oudemansin A
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Cytotoxicity against human KB cells by resazurin microplate assay
Cytotoxicity against human KB cells by resazurin microplate assay
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[PMID: 20329738] |
| MCF7 | IC50 |
>50 μg/mL
Compound: (-)-oudemansin A
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Cytotoxicity against human MCF7 cells by resazurin microplate assay
Cytotoxicity against human MCF7 cells by resazurin microplate assay
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[PMID: 20329738] |
| NCI-H187 | IC50 |
15.1 μg/mL
Compound: (-)-oudemansin A
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Cytotoxicity against human NCI-H187 cells by resazurin microplate assay
Cytotoxicity against human NCI-H187 cells by resazurin microplate assay
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[PMID: 20329738] |
| Vero | IC50 |
39.19 μg/mL
Compound: (-)-oudemansin A
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Cytotoxicity against african green monkey Vero cells by green fluorescent protein assay
Cytotoxicity against african green monkey Vero cells by green fluorescent protein assay
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[PMID: 20329738] |
Chemical Information
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CAS No. 73341-71-6
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Molecular Weight 290.35
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Formula C17H22O4
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SMILES
CO[C@@H](/C=C/C1=CC=CC=C1)[C@@H](C)/C(C(OC)=O)=C\OC
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Structure Classification
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Initial Source
Oudemansiella mucida
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
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BrdU Incorporation Assay
Bromodeoxyuridine (BrdU) incorporation assay is based on the principle that BrdU, a thymidine analog, is incorporated into newly synthesized DNA during the S phase of the cell cycle, thereby serving as a marker of DNA replication and cellular proliferation. Incorporated BrdU can be detected using anti-BrdU antibodies following DNA denaturation, enabling visualization or quantification of proliferating cells through immunochemical detection methods such as immunofluorescence or immunohistochemistry.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Oudemansin A Related Classifications
HY-165494 Related Classifications
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