Phorbol 12,13-dibutyrate (Synonyms: Phorbol dibutyrate; PDBu)

Cat. No.: HY-18985G: Data Sheet Handling Instructions
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Phorbol 12,13-dibutyrate (Phorbol dibutyrate) (GMP) is Phorbol 12,13-dibutyrate (HY-18985) produced by using GMP guidelines. GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. Phorbol 12,13-dibutyrate is a PKC activator and a potent skin tumor promoter.

For research use only. We do not sell to patients.

Phorbol 12,13-dibutyrate Chemical Structure

CAS No. : 37558-16-0

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
C3H 10T1/2	ED₅₀	0.02 μM Compound: PDBU	Tested for inhibition of EGF(epidermal growth factor) to C3H10T1/2 cells. Tested for inhibition of EGF(epidermal growth factor) to C3H10T1/2 cells.	[PMID: 10715158]
HEK293	EC₅₀	22.47 μM Compound: 2e	Activation of mouse wild type TRPV4 expressed in HEK293 cells assessed as increase in intracellular calcium concentration Activation of mouse wild type TRPV4 expressed in HEK293 cells assessed as increase in intracellular calcium concentration	[PMID: 19361196]
MT4	EC₅₀	14 nM Compound: 9	Antiviral activity against HIV2 ROD infected in MT4 cells assessed as cell viability after 5 days by MTT assay Antiviral activity against HIV2 ROD infected in MT4 cells assessed as cell viability after 5 days by MTT assay	[PMID: 25970561]
MT4	EC₅₀	79 nM Compound: 9	Antiviral activity against HIV1 3B infected in MT4 cells assessed as cell viability after 5 days by MTT assay Antiviral activity against HIV1 3B infected in MT4 cells assessed as cell viability after 5 days by MTT assay	[PMID: 25970561]
Vero	EC₅₀	1.8 μM Compound: 9	Antiviral activity against chikungunya virus Indian ocean strain 899 infected in Vero cells assessed as virus-induced cytopathic effect after 6 to 7 days Antiviral activity against chikungunya virus Indian ocean strain 899 infected in Vero cells assessed as virus-induced cytopathic effect after 6 to 7 days	[PMID: 25970561]

In Vitro

Phorbol 12,13-dibutyrate (Phorbol dibutyrate) (GMP) (1 μM) activates PKC and inhibited Na/K-ATPase transport activity in OK cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

504.61

Formula

C₂₈H₄₀O₈

CAS No.

37558-16-0

SMILES

O=C1C(C)=C[C@@]2([H])[C@]3(O)[C@@]([C@@](C4(C)C)([H])[C@@]4(OC(CCC)=O)[C@H](OC(CCC)=O)[C@H]3C)([H])C=C(CO)C[C@]12O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Singh J, et al. Immunocytochemical evidence for PDBu-induced activation of RhoA/ROCK in human internal anal sphincter smooth muscle cells. Am J Physiol Gastrointest Liver Physiol. 2011 Aug;301(2):G317-25. [Content Brief]

[2]. Szallasi Z, et al. Dissociation of phorbol esters leads to immediate redistribution to the cytosol of protein kinases C alpha and C delta in mouse keratinocytes. J Biol Chem. 1994 Nov 4;269(44):27159-62. [Content Brief]

[3]. Middleton JP, et al. Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells. J Biol Chem. 1993 Jul 25;268(21):15958-64. [Content Brief]

Phorbol 12,13-dibutyrate Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phorbol 12,13-dibutyrate (GMP)37558-16-0Phorbol dibutyrate (GMP)PDBu (GMP)PKCProtein kinase CInhibitorinhibitorinhibit

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