Phorbol 12,13-dibutyrate
Based on 3 publication(s) in Google Scholar
Phorbol 12,13-dibutyrate (Phorbol dibutyrate) is a PKC activator and a potent skin tumor promoter.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 37558-16-0
- Formula: C28H40O8
- Molecular Weight:504.61
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Phorbol 12,13-dibutyrate
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Customer Validation & Images
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WB
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Flow Cytometry
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WB
Biological Activity
PKC[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| C3H 10T1/2 | ED50 |
0.02 μM
Compound: PDBU
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Tested for inhibition of EGF(epidermal growth factor) to C3H10T1/2 cells.
Tested for inhibition of EGF(epidermal growth factor) to C3H10T1/2 cells.
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[PMID: 10715158] |
| HEK293 | EC50 |
22.47 μM
Compound: 2e
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Activation of mouse wild type TRPV4 expressed in HEK293 cells assessed as increase in intracellular calcium concentration
Activation of mouse wild type TRPV4 expressed in HEK293 cells assessed as increase in intracellular calcium concentration
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[PMID: 19361196] |
| MT4 | EC50 |
14 nM
Compound: 9
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Antiviral activity against HIV2 ROD infected in MT4 cells assessed as cell viability after 5 days by MTT assay
Antiviral activity against HIV2 ROD infected in MT4 cells assessed as cell viability after 5 days by MTT assay
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[PMID: 25970561] |
| MT4 | EC50 |
79 nM
Compound: 9
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Antiviral activity against HIV1 3B infected in MT4 cells assessed as cell viability after 5 days by MTT assay
Antiviral activity against HIV1 3B infected in MT4 cells assessed as cell viability after 5 days by MTT assay
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[PMID: 25970561] |
| Vero | EC50 |
1.8 μM
Compound: 9
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Antiviral activity against chikungunya virus Indian ocean strain 899 infected in Vero cells assessed as virus-induced cytopathic effect after 6 to 7 days
Antiviral activity against chikungunya virus Indian ocean strain 899 infected in Vero cells assessed as virus-induced cytopathic effect after 6 to 7 days
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[PMID: 25970561] |
Chemical Information
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CAS No. 37558-16-0
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Appearance Solid
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Molecular Weight 504.61
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Formula C28H40O8
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Color White to light yellow
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SMILES
O=C1C(C)=C[C@@]2([H])[C@]3(O)[C@@]([C@@](C4(C)C)([H])[C@@]4(OC(CCC)=O)[C@H](OC(CCC)=O)[C@H]3C)([H])C=C(CO)C[C@]12O
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Synonyms
Phorbol dibutyrate; PDBu
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (3)
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Journal Impact Factor
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Most Recent
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ACS Biomater Sci Eng
3D-Printed PCL Scaffolds Loaded with bFGF and BMSCs Enhance Tendon-Bone Healing in Rat Rotator Cuff Tears by Immunomodulation and Osteogenesis Promotion. [Abstract]2025 Feb 10;11(2):1123-1139. PMID: 39851055
Phorbol 12,13-dibutyrate purchased from MedChemExpress. Usage Cited in: ACS Biomater Sci Eng. 2025 Feb 10;11(2):1123-1139. [Abstract]
Phorbol 12,13-dibutyrate (PDBu) (10 μM; 2 h) treatment reversed the effect of PCLMF on the MAPK pathway in M1 macrophages.
Phorbol 12,13-dibutyrate purchased from MedChemExpress. Usage Cited in: ACS Biomater Sci Eng. 2025 Feb 10;11(2):1123-1139. [Abstract]
Phorbol 12,13-dibutyrate (PDBu) (10 μM; 2 h) reversed the effect of PCLMF on macrophage polarization.
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Front Cell Dev Biol
miR-212/132-Enriched Extracellular Vesicles Promote Differentiation of Induced Pluripotent Stem Cells Into Pancreatic Beta Cells. [Abstract]2021 May 13:9:673231. PMID: 34055806 -
Aging
Autophagy is induced in human keratinocytes during human papillomavirus 11 pseudovirion entry. [Abstract]2020 Nov 16;12(22):23017-23028. PMID: 33197887
Phorbol 12,13-dibutyrate purchased from MedChemExpress. Usage Cited in: Aging. 2020 Nov 16;12(22):23017-23028. [Abstract]
Phorbol 12,13-dibutyrate (PDBu) (1 μM; 30 min) restored the phosphorylation levels of Erk and partially rescued the phosphorylation levels of mTOR and ULK1 (S757), as well as the autophagy activity marker protein LC3-II.
Solvent & Solubility
DMSO : ≥ 125 mg/mL (247.72 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.12 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.12 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (805 KB)
- English - EN (805 KB)
- Français - FR (805 KB)
- Deutsch - DE (805 KB)
- Norwegian - NO (805 KB)
- Español - ES (805 KB)
- Swedish - SV (805 KB)
- Italian - IT (805 KB)
- Portuguese - PT (805 KB)
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Handling Instructions (2659 KB)
References
[1]. Singh J, et al. Immunocytochemical evidence for PDBu-induced activation of RhoA/ROCK in human internal anal sphincter smooth muscle cells. Am J Physiol Gastrointest Liver Physiol. 2011 Aug;301(2):G317-25. [Content Brief]
[2]. Szallasi Z, et al. Dissociation of phorbol esters leads to immediate redistribution to the cytosol of protein kinases C alpha and C delta in mouse keratinocytes. J Biol Chem. 1994 Nov 4;269(44):27159-62. [Content Brief]
[3]. Middleton JP, et al. Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells. J Biol Chem. 1993 Jul 25;268(21):15958-64. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9817 mL | 9.9086 mL | 19.8173 mL | 49.5432 mL |
| 5 mM | 0.3963 mL | 1.9817 mL | 3.9635 mL | 9.9086 mL | |
| 10 mM | 0.1982 mL | 0.9909 mL | 1.9817 mL | 4.9543 mL | |
| 15 mM | 0.1321 mL | 0.6606 mL | 1.3212 mL | 3.3029 mL | |
| 20 mM | 0.0991 mL | 0.4954 mL | 0.9909 mL | 2.4772 mL | |
| 25 mM | 0.0793 mL | 0.3963 mL | 0.7927 mL | 1.9817 mL | |
| 30 mM | 0.0661 mL | 0.3303 mL | 0.6606 mL | 1.6514 mL | |
| 40 mM | 0.0495 mL | 0.2477 mL | 0.4954 mL | 1.2386 mL | |
| 50 mM | 0.0396 mL | 0.1982 mL | 0.3963 mL | 0.9909 mL | |
| 60 mM | 0.0330 mL | 0.1651 mL | 0.3303 mL | 0.8257 mL | |
| 80 mM | 0.0248 mL | 0.1239 mL | 0.2477 mL | 0.6193 mL | |
| 100 mM | 0.0198 mL | 0.0991 mL | 0.1982 mL | 0.4954 mL |
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