Cat. No.: HY-N3075

Phytol

(Synonyms: (E)-Phytol)

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Phytol ((E)-Phytol) is an orally active diterpenoid alcohol that can be extracted from chlorophyll. Phytol has antioxidant, anti-inflammatory, anti-schistosomiasis and antibacterial activities.

For research use only. We do not sell to patients.

Purity: 99.97%
CAS No.: 150-86-7
Formula: C₂₀H₄₀O
Molecular Weight:296.53

Storage:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Phytol Chemical Structure

CAS No.: 150-86-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Phytol ((E)-Phytol) is an orally active diterpenoid alcohol that can be extracted from chlorophyll. Phytol has antioxidant, anti-inflammatory, anti-schistosomiasis and antibacterial activities^[1]^[2]^[3].

IC₅₀ & Target

Schistosome

All Parasite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>100 μM Compound: 5	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 25497963]
BMDC	IC₅₀	1.22 μM Compound: 19	Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated TNF-alpha production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated TNF-alpha production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA	[PMID: 23484668]
BMDC	IC₅₀	2.02 μM Compound: 19	Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated IL12 production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated IL12 production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA	[PMID: 23484668]
BMDC	IC₅₀	3.62 μM Compound: 19	Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated IL-12p40 production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated IL-12p40 production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA	[PMID: 23484668]
HCT-15	IC₅₀	22.5 μM Compound: 3	Antiproliferative activity against human HCT15 cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human HCT15 cells after 72 hrs by sulforhodamine B assay	[PMID: 25981689]
HeLa	IC₅₀	46.6 μM Compound: 6	Cytotoxicity against human HeLa cells by MTT method Cytotoxicity against human HeLa cells by MTT method	[PMID: 17547457]
HL-60	IC₅₀	>100 μM Compound: 5	Cytotoxicity against human HL60 cells by SRB assay Cytotoxicity against human HL60 cells by SRB assay	[PMID: 25497963]
K562	IC₅₀	45.8 μg/mL Compound: Phytol	Antiproliferative activity against human K562 cells assessed as reduction in cell viability incubated for 2 days by MTT assay Antiproliferative activity against human K562 cells assessed as reduction in cell viability incubated for 2 days by MTT assay	[PMID: 32527555]
MCF7	IC₅₀	>50 μg/mL Compound: 18	Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 21109433]
SW-620	IC₅₀	5.85 μM Compound: 3	Antiproliferative activity against human SW620 cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human SW620 cells after 72 hrs by sulforhodamine B assay	[PMID: 25981689]
U-251	IC₅₀	27.8 μM Compound: 3	Antiproliferative activity against human U251 cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human U251 cells after 72 hrs by sulforhodamine B assay	[PMID: 25981689]
U-87MG ATCC	IC₅₀	8.02 μM Compound: 3	Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay	[PMID: 25981689]

In Vitro

Phytol (50-100 μg/mL, 48 h) reduces the motor activity of worms and causes their death^[1].
Phytol (40 μM, 2 h) inhibits osteoclast differentiation and oxidative stress of RAW264.7 cells induced by RANKL by regulating Nrf2/HO-1^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

RAW264.7
Concentration:

5, 10, 20, 40 μM
Incubation Time:

5 days
Result:

Did not exert toxicity to RAW264.7.

Western Blot Analysis^[2]

Cell Line:

RAW264.7
Concentration:

40 μM
Incubation Time:

2 h
Result:

Results: Decreased the Nrf2 expression in the cytosolic extract and increased in the nuclear extract.
Did not affect the expression of the housekeeping genes, β-actin and lamin B.
Reduced the expression of NFATc1 and c-Fos.
Recovered the expression of SOD, CAT.

Cell Migration Assay ^[2]

Cell Line:

RAW264.7
Concentration:

5, 10, 20, 40 μM
Incubation Time:

5 days
Result:

Decreased cell migration and the bone resorption area and osteoclast movement.

In Vivo

Phytol (40 mg/kg, orally, for 2 weeks) can reduce the number of parasites in mice infected with adult C. mansoni^[1].
Phytol (10 or 20 mg/kg, orally, for 4 consecutive days) can improve anemia and oxidative brain damage in Plasmodium bergsii infected mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Plasmodium berghei infected mice^[3]
Dosage:

10 or 20 mg/kg
Administration:

p.o.
Result:

Suppressed the multiplication of P. berghei in a dose-dependent manner and ameliorated the P. berghei-induced anaemia and brain damage.

Chemical Information

CAS No. 150-86-7
Appearance Liquid (Density: 0.85 g/cm³)
Molecular Weight 296.53
Formula C₂₀H₄₀O
Color Light yellow to brown
SMILES

CC(C)CCC[C@@H](C)CCC[C@@H](C)CCC/C(C)=C/CO
Synonyms

(E)-Phytol
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (3)

All

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Journal Impact Factor

PLoS Biol

A high-throughput behavioral screening platform for measuring chemotaxis by C. elegans. [Abstract]

2024 Jun 27;22(6):e3002672. PMID: 38935621
Int J Environ Health Res

Green extraction and RSM optimization of Peganum harmala shoots for antioxidant and anti-inflammatory properties: modulation of pro-inflammatory cytokines. [Abstract]

2026 Feb 17:1-12. PMID: 41700732
bioRxiv

An efficient behavioral screening platform classifies natural products and other chemical cues according to their chemosensory valence in C. elegans. [Abstract]

2024 Apr 3:2023.06.02.542933. PMID: 37333363

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (337.23 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 25 mg/mL (84.31 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.3723 mL

16.8617 mL

33.7234 mL

5 mM

0.6745 mL

3.3723 mL

6.7447 mL

10 mM

0.3372 mL

1.6862 mL

3.3723 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.25 mg/mL (7.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.25 mg/mL (7.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.25 mg/mL (7.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. de Moraes J, et al. Phytol, a diterpene alcohol from chlorophyll, as a drug against neglected tropical diseaseSchistosomiasis mansoni. PLoS Negl Trop Dis. 2014 Jan 2;8(1):e2617. [Content Brief]

[2]. Kim EN, et al. Phytol Suppresses Osteoclast Differentiation and Oxidative Stress through Nrf2/HO-1 Regulation in RANKL-Induced RAW264.7 Cells. Cells. 2022 Nov 14;11(22):3596. [Content Brief]

[3]. Usman MA, et al. Phytol suppresses parasitemia and ameliorates anaemia and oxidative brain damage in mice infected with Plasmodium berghei. Exp Parasitol. 2021 May;224:108097. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol / DMSO	1 mM	3.3723 mL	16.8617 mL	33.7234 mL	84.3085 mL
	5 mM	0.6745 mL	3.3723 mL	6.7447 mL	16.8617 mL
	10 mM	0.3372 mL	1.6862 mL	3.3723 mL	8.4309 mL
	15 mM	0.2248 mL	1.1241 mL	2.2482 mL	5.6206 mL
	20 mM	0.1686 mL	0.8431 mL	1.6862 mL	4.2154 mL
	25 mM	0.1349 mL	0.6745 mL	1.3489 mL	3.3723 mL
	30 mM	0.1124 mL	0.5621 mL	1.1241 mL	2.8103 mL
	40 mM	0.0843 mL	0.4215 mL	0.8431 mL	2.1077 mL
	50 mM	0.0674 mL	0.3372 mL	0.6745 mL	1.6862 mL
	60 mM	0.0562 mL	0.2810 mL	0.5621 mL	1.4051 mL
	80 mM	0.0422 mL	0.2108 mL	0.4215 mL	1.0539 mL
DMSO	100 mM	0.0337 mL	0.1686 mL	0.3372 mL	0.8431 mL

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Phytol

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Publications Citing Use of MedChemExpress (MCE) Phytol

All Parasite Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Phytol Related Classifications

HY-N3075 Related Classifications