2. Anti-infection NF-κB
  3. Parasite Bacterial Keap1-Nrf2
  4. Phytol

Phytol  (Synonyms: (E)​-​Phytol)

Cat. No.: HY-N3075 Purity: ≥98.0%
COA Handling Instructions

Molarity Calculator

Phytol ((E)-Phytol) is an orally active diterpenoid alcohol that can be extracted from chlorophyll. Phytol has antioxidant, anti-inflammatory, anti-schistosomiasis and antibacterial activities.

For research use only. We do not sell to patients.

Phytol Chemical Structure

Phytol Chemical Structure

CAS No. : 150-86-7

Size Price Stock Quantity
Oil + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Oil
1 g USD 50 In-stock
5 g   Get quote  
10 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Phytol Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Phytol

View All Parasite Isoform Specific Products:

View All Isoforms
Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Phytol ((E)-Phytol) is an orally active diterpenoid alcohol that can be extracted from chlorophyll. Phytol has antioxidant, anti-inflammatory, anti-schistosomiasis and antibacterial activities[1][2][3].

IC50 & Target

Schistosome

 

In Vitro

Phytol (50-100 μg/mL, 48 h) reduces the motor activity of worms and causes their death[1].
Phytol (40 μM, 2 h) inhibits osteoclast differentiation and oxidative stress of RAW264.7 cells induced by RANKL by regulating Nrf2/HO-1[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Phytol Related Antibodies

Cell Viability Assay[2]

Cell Line: RAW264.7
Concentration: 5, 10, 20, 40 μM
Incubation Time: 5 days
Result: Did not exert toxicity to RAW264.7.

Western Blot Analysis[2]

Cell Line: RAW264.7
Concentration: 40 μM
Incubation Time: 2 h
Result: Results: Decreased the Nrf2 expression in the cytosolic extract and increased in the nuclear extract.
Did not affect the expression of the housekeeping genes, β-actin and lamin B.
Reduced the expression of NFATc1 and c-Fos.
Recovered the expression of SOD, CAT.

Cell Migration Assay [2]

Cell Line: RAW264.7
Concentration: 5, 10, 20, 40 μM
Incubation Time: 5 days
Result: Decreased cell migration and the bone resorption area and osteoclast movement.
In Vivo

Phytol (40 mg/kg, orally, for 2 weeks) can reduce the number of parasites in mice infected with adult C. mansoni[1].
Phytol (10 or 20 mg/kg, orally, for 4 consecutive days) can improve anemia and oxidative brain damage in Plasmodium bergsii infected mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Plasmodium berghei infected mice[3]
Dosage: 10 or 20 mg/kg
Administration: p.o.
Result: Suppressed the multiplication of P. berghei in a dose-dependent manner and ameliorated the P. berghei-induced anaemia and brain damage.
Molecular Weight

296.53

Formula

C20H40O
CAS No.
Appearance

Oil

Color

Light brown to brown

SMILES

CC(C)CCC[C@@H](C)CCC[C@@H](C)CCC/C(C)=C/CO
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (421.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 25 mg/mL (84.31 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3723 mL 16.8617 mL 33.7234 mL
5 mM 0.6745 mL 3.3723 mL 6.7447 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.25 mg/mL (7.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.25 mg/mL (7.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 3.3723 mL 16.8617 mL 33.7234 mL 84.3085 mL
5 mM 0.6745 mL 3.3723 mL 6.7447 mL 16.8617 mL
10 mM 0.3372 mL 1.6862 mL 3.3723 mL 8.4309 mL
15 mM 0.2248 mL 1.1241 mL 2.2482 mL 5.6206 mL
20 mM 0.1686 mL 0.8431 mL 1.6862 mL 4.2154 mL
25 mM 0.1349 mL 0.6745 mL 1.3489 mL 3.3723 mL
30 mM 0.1124 mL 0.5621 mL 1.1241 mL 2.8103 mL
40 mM 0.0843 mL 0.4215 mL 0.8431 mL 2.1077 mL
50 mM 0.0674 mL 0.3372 mL 0.6745 mL 1.6862 mL
60 mM 0.0562 mL 0.2810 mL 0.5621 mL 1.4051 mL
80 mM 0.0422 mL 0.2108 mL 0.4215 mL 1.0539 mL
DMSO 100 mM 0.0337 mL 0.1686 mL 0.3372 mL 0.8431 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Phytol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phytol150-86-7(E)​-​PhytolParasiteBacterialKeap1-Nrf2Anti-inflammatoryAntioxidantRAW264.7Plasmodium bergheiInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Phytol
Cat. No.:
HY-N3075
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.