PLP (180-199) TFA
Based on 1 Customer Validation
PLP (180-199) TFA is a myelin proteolipid protein-derived peptide corresponding to amino acids 180-199 of PLP. PLP (180-199) TFA acts as an encephalitogenic peptide and induces experimental autoimmune encephalomyelitis (EAE) in susceptible BALB/c mice. PLP (180-199) TFA can stimulate antigen-specific T-cell responses, promote inflammatory cytokine production including IFN-γ and TNF-α, and is used for the study of autoimmune encephalomyelitis and multiple sclerosis-related immune responses.
For research use only. We do not sell to patients.
- Purity: 99.22%
- Formula: C92H145N23O30S.xC2HF2O3
- Molecular Weight:2085.34 (free base)
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
PLP (180-199) TFA stimulates antigen-specific lymphocyte proliferation in spleen and lymph node cells isolated from PLP180-199-immunized BALB/c mice after peptide restimulation[1].
PLP (180-199) (10 μg/mL) TFA induces IFN-γ and TNF-α production in lymphoid cells isolated from PLP180-199-immunized BALB/c mice after peptide stimulation[2].
PLP (180-199) (10-14 days after immunization) TFA stimulates proliferation and IFN-γ production of spleen and lymph node cells from wild-type and B cell-deficient BALB/c mice after PLP180-199 immunization[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
PLP (180-199) (200 μg/mouse; s.c. immunization) TFA induces EAE in BALB/c mice with increased clinical disease scores and characteristic ascending paralysis[2].
PLP (180-199) (200 μg/mouse; s.c. immunization) TFA induces EAE in wild-type and B cell-deficient BALB/c mice and regulates disease severity through antigen-specific immune responses[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.
Administration: PLP (180-199) (200 μg/mouse) TFA peptide emulsified 1:1 in incomplete Freund’s adjuvant (IFA) containing Mycobacterium tuberculosis H37Ra (0.5 mg/mouse; 0.1 mL emulsion/mouse)•administered subcutaneously (s.c.) at immunization sites. Pertussis toxin (300 ng/mouse) was injected intravenously (i.v.) at the time of immunization and 72 h later to induce EAE
(2) Pertussis toxin administration is required for EAE induction and was given intravenously at immunization and 72 h later.
(3) Clinical EAE severity was evaluated using a clinical scoring system based on paralysis progression.
Histology analysis: Inflammatory cell infiltration, demyelination, and axonal loss in the central nervous system.
Immune response analysis:Increased PLP-specific T cell responses and altered cytokine production, including IFN-γ and IL-10 changes.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 2085.34 (free base)
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Formula C92H145N23O30S.xC2HF2O3
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Color White to off-white
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Sequence
Trp-Thr-Thr-Cys-Gln-Ser-Ile-Ala-Phe-Pro-Ser-Lys-Thr-Ser-Ala-Ser-Ile-Gly-Ser-Leu
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Sequence Shortening
WTTCQSIAFPSKTSASIGSL
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 25 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 10 mg/mL (Need ultrasonic)
Purity & Documentation
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Data Sheet (288 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PLP (180-199) TFA Related Classifications
HY-P5462A Related Classifications
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