2. Immunology/Inflammation Vitamin D Related/Nuclear Receptor Stem Cell/Wnt Neuronal Signaling
  3. Glucocorticoid Receptor Interleukin Related Notch
  4. Prednisone acetate

Prednisone acetate  (Synonyms: Prednisone 21-acetate)

Cat. No.: HY-B1832 Purity: 99.71%
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Prednisone acetate (Prednisone 21-acetate), a glucocorticoid, is an orally active Notch inhibitor. Prednisone acetate has anti-inflammatory activity and can enhance the immune response.

For research use only. We do not sell to patients.

Prednisone acetate Chemical Structure

Prednisone acetate Chemical Structure

CAS No. : 125-10-0

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Other Forms of Prednisone acetate:

Prednisone acetate Related Antibodies

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Description

Prednisone acetate (Prednisone 21-acetate), a glucocorticoid, is an orally active Notch inhibitor. Prednisone acetate has anti-inflammatory activity and can enhance the immune response[1][2].

IC50 & Target

IL-17

 

IL-10

 

IL-4

 

In Vivo

Prednisone acetate (5 mg/kg, Intragastric gavage, once a day for 4 weeks) induces hippocampal LTP impairment by causing neuronal lesions in the dentate gyrus, which reduced glutamic acid (Glu) and NMDAR2A, and impaired spatial memory in Hippocampal LTP impairment model mice[1].
Prednisone acetate (6 mg/kg, gavage, once a day for 15 days) reduces ocular and peripheral inflammatory responses and restores Th1/Th2 and Th17/Treg immune homeostasis through orchestrating the Notch signaling pathway in Experimental autoimmune uveitis (EAU) rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice [1]
Dosage: 5 mg/kg
Administration: Intragastric gavage (i.g.)
Result: Significantly failed to gain weight.
Decreased the population spike (%) after high-frequency stimulation.
Reduced the crossing times compared with the control group.
Resulted in the impairment of memory, and caused a trend of more serious impairment.
Reduced the levels of Glu and gamma-aminobutyric acid (GABA) in the hippocampus.
Reduced the expressions of N-methyl-D-aspartate receptors in the hippocampus.
Animal Model: Experimental autoimmune uveitis [2]
Dosage: 6 mg/kg
Administration: gavage
Result: Showed fewer manifestations in eyes from day 9 after immunization.
Reduced the inflammation in EAU rats.
Bound with the pocket of Notch signaling-related molecules with good affinity.
Exhibited anti-inflammatory effects through inhibiting Notch signaling activation.
Decreased Th1, Th17 and increased Th2, Treg frequencies in EAU.
Clinical Trial
Molecular Weight

400.46

Formula

C23H28O6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]1(C2)[C@](C(COC(C)=O)=O)(O)CC[C@@]1([H])[C@]3([H])CCC4=CC(C=C[C@]4(C)[C@@]3([H])C2=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (62.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4971 mL 12.4856 mL 24.9713 mL
5 mM 0.4994 mL 2.4971 mL 4.9943 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.24 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (5.19 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.71%

COA (252 KB) HNMR (274 KB) LCMS (268 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4971 mL 12.4856 mL 24.9713 mL 62.4282 mL
5 mM 0.4994 mL 2.4971 mL 4.9943 mL 12.4856 mL
10 mM 0.2497 mL 1.2486 mL 2.4971 mL 6.2428 mL
15 mM 0.1665 mL 0.8324 mL 1.6648 mL 4.1619 mL
20 mM 0.1249 mL 0.6243 mL 1.2486 mL 3.1214 mL
25 mM 0.0999 mL 0.4994 mL 0.9989 mL 2.4971 mL
30 mM 0.0832 mL 0.4162 mL 0.8324 mL 2.0809 mL
40 mM 0.0624 mL 0.3121 mL 0.6243 mL 1.5607 mL
50 mM 0.0499 mL 0.2497 mL 0.4994 mL 1.2486 mL
60 mM 0.0416 mL 0.2081 mL 0.4162 mL 1.0405 mL
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Prednisone acetate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Prednisone125-10-0Prednisone 21-acetateGlucocorticoid ReceptorInterleukin RelatedNotchILHippocampusExperimental autoimmune uveitisInhibitorinhibitorinhibit

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