Rifamycin B
Based on 1 Customer Validation
Rifamycin B (4-O-(Carboxymethyl)-Rifamycin) is a member of ansamycin antibiotics family with an anti-mycobacterial activity against tuberculosis, leprosy and AIDS-related mycobacterial infections. Rifamycin B is a metabolic product of Nocardia inediterranei, which can yield Rifamycin O and Rifamycin S (HY-125365) by microbial transformation.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 13929-35-6
- Formula: C39H49NO14
- Molecular Weight:755.80
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Chemical Information
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CAS No. 13929-35-6
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Appearance Solid
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Molecular Weight 755.80
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Formula C39H49NO14
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Color Light yellow to yellow
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SMILES
C/C(C(N1)=O)=C/C=C/[C@H](C)[C@H](O)[C@@H](C)[C@@H](O)[C@@H](C)[C@H](OC(C)=O)[C@@H]([C@@H](OC)/C=C/O[C@]2(C)C(C3=C(O2)C(C)=C(O)C4=C3C(OCC(O)=O)=CC1=C4O)=O)C
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Synonyms
4-O-(Carboxymethyl)-Rifamycin
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (132.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (3.31 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Seong BL, et al. Microbial transformation of rifamycin B: a new synthetic approach to rifamycin derivatives. J Antibiot (Tokyo). 1983 Oct;36(10):1402-4. [Content Brief]
[2]. Jin ZH, et al. Scale-up of rifamycin B fermentation with Amycolatoposis mediterranei. J Zhejiang Univ Sci. 2004 Dec;5(12):1590-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3231 mL | 6.6155 mL | 13.2310 mL | 33.0775 mL |
| 5 mM | 0.2646 mL | 1.3231 mL | 2.6462 mL | 6.6155 mL | |
| 10 mM | 0.1323 mL | 0.6616 mL | 1.3231 mL | 3.3078 mL | |
| 15 mM | 0.0882 mL | 0.4410 mL | 0.8821 mL | 2.2052 mL | |
| 20 mM | 0.0662 mL | 0.3308 mL | 0.6616 mL | 1.6539 mL | |
| 25 mM | 0.0529 mL | 0.2646 mL | 0.5292 mL | 1.3231 mL | |
| 30 mM | 0.0441 mL | 0.2205 mL | 0.4410 mL | 1.1026 mL | |
| 40 mM | 0.0331 mL | 0.1654 mL | 0.3308 mL | 0.8269 mL | |
| 50 mM | 0.0265 mL | 0.1323 mL | 0.2646 mL | 0.6616 mL | |
| 60 mM | 0.0221 mL | 0.1103 mL | 0.2205 mL | 0.5513 mL | |
| 80 mM | 0.0165 mL | 0.0827 mL | 0.1654 mL | 0.4135 mL | |
| 100 mM | 0.0132 mL | 0.0662 mL | 0.1323 mL | 0.3308 mL |
Rifamycin B Related Classifications
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