Sampsonione P
Sampsonione P is an RXRα inhibitor isolated and extracted from Hypericum sampsonii. Sampsonione P inhibits RXRα transcriptional activity and can downregulate the expression of pro-inflammatory proteins such as iNOS and COX-2, thereby exerting anti-inflammatory effects. Sampsonione P has antitumor activity. Sampsonione P can be used for research on inflammatory diseases and cancer.
For research use only. We do not sell to patients.
- CAS No.: 1562672-80-3
- Formula: C33H42O5
- Molecular Weight:518.68
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
Description
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RXR α |
COX-2 |
iNOS |
In Vitro
The compound class including Sampsonione P (compound 6), specifically compounds 6-9, exhibits dose-dependent cytotoxicity against HeLa cells at concentrations of 5-20 μM[2].
Sampsonione P (50 μM; 24 h) exhibits cytotoxic effects on RAW264.7 cells, reducing cell viability to 46.29%[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:RAW264.7 cells
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Concentration:50 μM
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Incubation Time:24 h
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Result:Reduced cell viability to 46.29%.
Chemical Information
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CAS No. 1562672-80-3
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Molecular Weight 518.68
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Formula C33H42O5
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SMILES
O=C1[C@@]23C(O[C@@H](C3)C(C)(O)C)=C(C([C@@]1(C(C)([C@@H](C2)C/C=C(C)\C)C)C/C=C(C)\C)=O)C(C4=CC=CC=C4)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Dual Luciferin reporter gene assay
Luciferin reporter gene assay is a reporting system to detect the activity of Firefly Luciferase using luciferin as a substrate, which is often used in the research of miRNA target gene verification and promoter transcriptive activity regulation. Dual luciferase usually refers to Firefly luciferase and Renilla luciferase.
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
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Subcutaneous Cell-Line-Derived Xenograft
Subcutaneous cell-line-derived xenograft (CDX) models are established by implanting cultured human cancer cell lines into immunodeficient mice, where the injected cells form localized tumors that can be monitored in vivo as a measure of tumorigenic potential, growth kinetics, and treatment response. These models are widely used in oncology research because they allow reproducible tumor formation and enable comparative assessment of tumor growth between different cell lines or genetic manipulations in a controlled in vivo microenvironment. Subcutaneous implantation of cancer cells in immunodeficient mice is a standard approach for evaluating tumor growth behavior and therapeutic response across multiple cancer types, including prostate, esophageal, pancreatic, and colon cancer models.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Sampsonione P Related Classifications
HY-N21705 Related Classifications
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Keywords
- Sampsonione P
- 1562672-80-3
- RAR/RXR
- COX
- NO Synthase
- Endogenous Metabolite
- RAW264.7 cell viability
- RXRα transcriptional activity
- iNOS
- RXRα inhibitor
- leukaemia and lung cancer
- LPS-induced nitric oxide production
- HeLa cell viability
- COX-2 protein expression
- human cancer cell lines
- LPS-activated RAW264.7 cells
- Inhibitor
- inhibitor
- inhibit