Paradol
Based on 4 publication(s) in Google Scholar
Paradol is a pungent phenolic substance found in ginger and other Zingiberaceae plants. Paradol is an effective inhibitor of tumor promotion in mouse skin carcinogenesis, binds to cyclooxygenase (COX)-2 active site.
For research use only. We do not sell to patients.
- Purity: 98.66%
- CAS No.: 27113-22-0
- Formula: C17H26O3
- Molecular Weight:278.39
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
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Publications Citing Use of MedChemExpress (MCE) Paradol
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Biological Activity
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COX-2 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
84 μM
Compound: M11
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Cytotoxicity against human HCT116 cells assessed as growth inhibition after 24 hrs by MTT assay
Cytotoxicity against human HCT116 cells assessed as growth inhibition after 24 hrs by MTT assay
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[PMID: 22246389] |
| NCI-H1299 | IC50 |
66.5 μM
Compound: M11
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Cytotoxicity against human H1299 cells assessed as growth inhibition after 24 hrs by MTT assay
Cytotoxicity against human H1299 cells assessed as growth inhibition after 24 hrs by MTT assay
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[PMID: 22246389] |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 27113-22-0
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Appearance Liquid (Density: 1.0690 g/cm3)
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Molecular Weight 278.39
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Formula C17H26O3
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Color Light yellow to yellow
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SMILES
COC1=C(O)C=CC(CCC(CCCCCCC)=O)=C1
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Synonyms
[6]-Gingerone; [6]-Paradol
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (4)
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Journal Impact Factor
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Most Recent
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ChemMedChem
Machine Learning-Driven Discovery of Structurally Related Natural Products as Activators of the Cardiac Calcium Pump SERCA2a. [Abstract]2025 Jan 23:e202400913. PMID: 39853697 -
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Solvent & Solubility
DMSO : ≥ 140 mg/mL (502.89 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
KB, human oral epidermoid carcinoma cell lines (ATCC CCL-17) are plated at a density of 5×103 cells/200 μL/well into 96-well plate. After an overnight growth, the cells are treated with a series of paradol derivatives. All of the derivatives of paradol tested are dissolved in DMSO. The final concentration of DMSO in the culture medium is kept below 0.1% and the controls are treated with DMSO alone. Cell viability is assessed using MTT assay. In brief, after the cells are grown in the media in the absence or presence of the test compounds (e.g., Paradol, 10, 50, 100, 150, and 200 μM) for 48 h, they are then replaced to a 200 μL culture medium containing 0.5 mg/mL MTT for 3 h. The resulting MTT-formazan product is dissolved by an addition of the same volume of DMSO. The amount of formazan is determined by measuring the absorbance at 570 nm[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[3]
Male ICR mice (7 weeks old, 36±2 g) challenged with middle cerebral artery occlusion (MCAO)/reperfusion (M/R) are randomly divided into vehicle (10% Tween80)- or Paradol-administered groups (n=6~7 per group). Paradol dissolved in 10% Tween80 is orally administered (10 mg/kg) into mice at 1, 5, or 10 mg/kg immediately after reperfusion.
Rats[4]
Five-week-old Sprague-Dawley rats (male) are used. At 8 weeks of age, the rats are fasted for 14 h prior to the oral administration of olive oil (1 mL) containing zingerone or 6-, 8-, or 12-paradol (10 mg/kg). Three rats in each group are anesthetized with isoflurane, and samples (0.3 mL) of their blood are collected from their jugular vein using a heparinized needle and syringe at 0 (i.e., prior to the oral administration), 0.25, 0.5, 1, 3, 6, and 24 h after the oral administration of the olive oil containing test compounds. The AUC0-24h values determined using this time schedule are very similar compared with AUC0-24h that sampled the time points more minutely with other materials in our laboratory.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. van Breemen RB, et al. Cyclooxygenase-2 inhibitors in ginger (Zingiber officinale). Fitoterapia. 2011 Jan;82(1):38-43. [Content Brief]
[2]. Keum YS, et al. Induction of apoptosis and caspase-3 activation by chemopreventive [6]-paradol and structurally related compounds in KB cells. Cancer Lett. 2002 Mar 8;177(1):41-7. [Content Brief]
[3]. Gaire BP, et al. Neuroprotective effect of 6-paradol in focal cerebral ischemia involves the attenuation of neuroinflammatory responses in activated microglia. PLoS One. 2015 Mar 19;10(3):e0120203. [Content Brief]
[4]. Setoguchi S, et al. Pharmacokinetics of Paradol Analogues Orally Administered to Rats. J Agric Food Chem. 2016 Mar 9;64(9):1932-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5921 mL | 17.9604 mL | 35.9208 mL | 89.8021 mL |
| 5 mM | 0.7184 mL | 3.5921 mL | 7.1842 mL | 17.9604 mL | |
| 10 mM | 0.3592 mL | 1.7960 mL | 3.5921 mL | 8.9802 mL | |
| 15 mM | 0.2395 mL | 1.1974 mL | 2.3947 mL | 5.9868 mL | |
| 20 mM | 0.1796 mL | 0.8980 mL | 1.7960 mL | 4.4901 mL | |
| 25 mM | 0.1437 mL | 0.7184 mL | 1.4368 mL | 3.5921 mL | |
| 30 mM | 0.1197 mL | 0.5987 mL | 1.1974 mL | 2.9934 mL | |
| 40 mM | 0.0898 mL | 0.4490 mL | 0.8980 mL | 2.2451 mL | |
| 50 mM | 0.0718 mL | 0.3592 mL | 0.7184 mL | 1.7960 mL | |
| 60 mM | 0.0599 mL | 0.2993 mL | 0.5987 mL | 1.4967 mL | |
| 80 mM | 0.0449 mL | 0.2245 mL | 0.4490 mL | 1.1225 mL | |
| 100 mM | 0.0359 mL | 0.1796 mL | 0.3592 mL | 0.8980 mL |
Paradol Related Classifications
HY-14617 Related Classifications
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