Cat. No.: HY-13812

QNZ

(Synonyms: EVP4593)

49 Cited Publications

Customer Review

Based on 49 publication(s) in Google Scholar

QNZ (EVP4593) shows strong inhibitory effects on NF-κB transcriptional activation and TNF-α production with IC₅₀s of 11 and 7 nM, respectively. QNZ (EVP4593) is a neuroprotective inhibitor of SOC channel.

For research use only. We do not sell to patients.

Purity: 99.50%
CAS No.: 545380-34-5
Formula: C₂₂H₂₀N₄O
Molecular Weight:356.42

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

QNZ Chemical Structure

CAS No.: 545380-34-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (49)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

NF-κB

11 nM (IC₅₀, in human Jurkat cells transfected with pNFκB-Luc)

All NF-κB Isoforms :

All TNF Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	IC₅₀	0.012 μM Compound: 99302782	HARVARD: Inhibition of liver stage Plasmodium berghei infection in HepG2 cells HARVARD: Inhibition of liver stage Plasmodium berghei infection in HepG2 cells	[PMID: 22586124]
HepG2	IC₅₀	59 μM Compound: 99302782	HARVARD: Cytotoxicity in HepG2 cell line HARVARD: Cytotoxicity in HepG2 cell line	[PMID: 22586124]

In Vitro

QNZ (Compound 11q) has a suppressing effect of the NF-κB mediated-inflammatory response. QNZ inhibits edema formation dose-dependently^[1]. QNZ (EVP4593) reduces the number of lysosomes/autophagosomes and store-operated channel (SOC) currents in Huntington's disease (HD). Normalization of calcium transport within neurons in response to QNZ is expect to reduce pathology manifestation. A number of lysosomes/autophagosomes are evaluated in HD and WT neurons treated with QNZ using transmission electron microscopy (TEM). Incubation with QNZ reduces the number of lysosomes/autophagosomes in HD GABAergic medium spiny (GABA MS)-like neurons (GMSLNs) by almost two-fold (from 0.41±0.04 to 0.23±0.04; p<0.05), while WT neurons are not affected. This observation is confirmed by examining lysosome content by flow cytometry (FC) analysis. The median fluorescence intensity is reduced by 34±6 % in HD GMSLNs upon QNZ treatment (p<0.05)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 545380-34-5
Appearance Solid
Molecular Weight 356.42
Formula C₂₂H₂₀N₄O
Color Light green to green
SMILES

NC1=CC2=C(NCCC3=CC=C(OC4=CC=CC=C4)C=C3)N=CN=C2C=C1
Synonyms

EVP4593
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (49)

All WB

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Journal Impact Factor

Signal Transduct Target Ther

Piezo1 activation suppresses bone marrow adipogenesis to prevent osteoporosis by inhibiting a mechanoinflammatory autocrine loop. [Abstract]

2025 Oct 28;10(1):357. PMID: 41145414
Cancer Cell

Overcoming tyrosine kinase inhibitor resistance in lung cancer brain metastasis with CTLA4 blockade. [Abstract]

2024 Nov 11;42(11):1882-1897.e7. PMID: 39423817
Nat Commun

Phenazine biosynthesis-like domain-containing protein (PBLD) and Cedrelone promote antiviral immune response by activating NF-ĸB. [Abstract]

2025 Jan 8;16(1):496. PMID: 39779683
Cell Death Differ

Transcription factor Zhx2 is a checkpoint that programs macrophage polarization and antitumor response. [Abstract]

2023 Sep;30(9):2104-2119. PMID: 37582865
Adv Sci (Weinh)

Glutamine Metabolism Underlies the Functional Similarity of T Cells between Nile Tilapia and Tetrapod. [Abstract]

2023 Apr;10(12):e2201164. PMID: 36890649
J Adv Res

TRPM2-mediated feed-forward loop promotes chondrocyte damage in osteoarthritis via calcium-cGAS-STING-NF-κB pathway. [Abstract]

2024 Nov 5:S2090-1232(24)00499-5. PMID: 39505144
Cell Mol Biol Lett

Mesenchymal stem cell-secreted KGF ameliorates acute lung injury via the Gab1/ERK/NF-κB signaling axis. [Abstract]

2025 Jul 10;30(1):79. PMID: 40640718
Cancer Lett

Exosomes deliver lncRNA DARS-AS1 siRNA to inhibit chronic unpredictable mild stress-induced TNBC metastasis. [Abstract]

2022 Sep 1:543:215781. PMID: 35688263
Cell Death Dis

RANKL/RANK signaling recruits Tregs via the CCL20-CCR6 pathway and promotes stemness and metastasis in colorectal cancer. [Abstract]

2024 Jun 20;15(6):437. PMID: 38902257
Cancer Immunol Res

Blocking WNT7A Enhances MHC-I Antigen Presentation and Enhances the Effectiveness of Immune Checkpoint Blockade Therapy. [Abstract]

2025 Mar 4;13(3):400-416. PMID: 39602462
Haematologica

MicroRNA-21 maintains hematopoietic stem cell homeostasis through sustaining the NF-κB signaling pathway in mice. [Abstract]

2020 Jan 23;haematol.2019.236927. PMID: 31974197
Clin Sci (Lond)

Ergosterol attenuates cigarette smoke extract-induced COPD by modulating inflammation, oxidative stress and apoptosis in vitro and in vivo. [Abstract]

2019 Jul 15;133(13):1523-1536. PMID: 31270147

QNZ purchased from MedChemExpress. Usage Cited in: Clin Sci (Lond). 2019 Jul 15;133(13):1523-1536. [Abstract]

Western blot analyses show that the inhibition of NF-κB/p65 enhanced the protective effect of ergosterol in CSE-induced 16HBE cells.
Mucosal Immunol

Targeting the EP2 receptor ameliorates inflammatory bowel disease in mice by enhancing the immunosuppressive activity of Treg cells. [Abstract]

2025 Apr;18(2):418-430. PMID: 39746548
Biomed Pharmacother

Mesenchymal stem cell conditioned medium alleviates acute lung injury through KGF-mediated regulation of epithelial sodium channels. [Abstract]

2023 Dec 31:169:115896. PMID: 37984305
Environ Pollut

Procymidone induces muscle atrophy and impaired myocyte fusion via the TNF-α/NF-κB/ROS pathway in developing zebrafish embryos. [Abstract]

2026 Apr 1:394:127760. PMID: 41638287
Mol Med

Dysregulated lipid metabolism and intervertebral disc degeneration: the important role of ox-LDL/LOX-1 in endplate chondrocyte senescence and calcification. [Abstract]

2024 Aug 9;30(1):117. PMID: 39123116
Ecotoxicol Environ Saf

Asiaticoside ameliorates pyrimethanil-induced autophagy-dependent liver injury by suppressing CRHR1. [Abstract]

2025 Dec 2:308:119482. PMID: 41338089
Front Immunol

Targeted Inhibition of FTO Demethylase Protects Mice Against LPS-Induced Septic Shock by Suppressing NLRP3 Inflammasome. [Abstract]

2021 May 4:12:663295. PMID: 34017338
J Mol Cell Biol

GSDMB promotes non-canonical pyroptosis by enhancing caspase-4 activity. [Abstract]

2019 Jun 1;11(6):496-508. PMID: 30321352

QNZ purchased from MedChemExpress. Usage Cited in: J Mol Cell Biol. 2019 Jun 1;11(6):496-508. [Abstract]

Cytoplasmic LPS-induced GSDMB and GSDMD expression are both significantly attenuated by either JSH-23 or QNZ.
Int J Mol Med

Long non‑coding RNA CASC2 ameliorates sepsis‑induced acute kidney injury by regulating the miR‑155 and NF‑κB pathway. [Abstract]

2020 May;45(5):1554-1562. PMID: 32323747
Am J Chin Med

Maslinic Acid Ameliorates Myocardial Ischemia Reperfusion Injury-Induced Oxidative Stress via Activating Nrf2 and Inhibiting NF-[Formula: see text]B Pathways. [Abstract]

2023;51(4):929-951. PMID: 36974993
J Pathol

LncRNA GAS5 ameliorates intestinal barrier injury by targeting the miR-223-3p/FBXW7 axis and inactivating NF-κB signaling in vitro and in vivo. [Abstract]

2025 Sep;267(1):25-39. PMID: 40719162
Drug Des Devel Ther

Design, Synthesis, and Biological Evaluation of Novel Hydroxytyrosol Derivatives as Protectors for Vascular Endothelium Against Lipid Overload. [Abstract]

2025 Apr 1:19:2433-2452. PMID: 40190807
Respir Res

Submersion and hypoxia inhibit alveolar epithelial Na+ transport through ERK/NF-κB signaling pathway. [Abstract]

2023 Apr 24;24(1):117. PMID: 37095538
Biochim Biophys Acta Mol Basis Dis

Dengue virus is involved in insulin resistance via the downregulation of IRS-1 by inducing TNF-α secretion. [Abstract]

2022 Oct 1;1868(10):166472. PMID: 35752384
FASEB J

Fish NF-κB couples TCR and IL-17 signals to regulate ancestral T-cell immune response against bacterial infection. [Abstract]

2021 Apr;35(4):e21457. PMID: 33689192
Mol Nutr Food Res

Lactobacillus Casei Zhang Alleviates Shrimp Tropomyosin-Induced Food Allergy by Switching Antibody Isotypes through the NF-κB-Dependent Immune Tolerance. [Abstract]

2020 May;64(10):e1900496. PMID: 32243079
J Cell Mol Med

The outer membrane protein Tp92 of Treponema pallidum induces human mononuclear cell death and IL-8 secretion. [Abstract]

2018 Dec;22(12):6039-6054. PMID: 30596396

QNZ purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2018 Dec;22(12):6039-6054. [Abstract]

NF-κB protein expression in cells treated with Tp92 (5 μg/mL) or Tp92 (5 μg/mL)+QNZ (10 nM).
J Funct Foods

2023 Nov, 110, 105863.
J Funct Foods

2023 Jun, 105, 105582.
Fish Shellfish Immunol

Characteristics of CD4-1 gene and its immune responses against Aeromonas veronii infection by activating NF-κB signaling in Qihe crucian carp Carassius auratus. [Abstract]

2024 Feb:145:109318. PMID: 38142019
Fish Shellfish Immunol

Diflovidazin damages the hematopoietic stem cells to zebrafish embryos via the TLR4/ NF-κB/ p53 pathway. [Abstract]

2023 Apr:135:108672. PMID: 36893927
Aging

Astaxanthin protects against osteoarthritis via Nrf2: a guardian of cartilage homeostasis. [Abstract]

2019 Nov 26;11(22):10513-10531. PMID: 31772142
Cell Signal

TFAP2A-mediated downregulation of UBE2C is crucial for endometrial decidualization and embryo implantation. [Abstract]

2025 Nov:135:112016. PMID: 40701315
Mol Cell Endocrinol

Elevated latent transforming growth factor beta binding protein 2 in endometriosis promotes endometrial stromal cell invasion and proliferation via the NF-kB signaling pathway. [Abstract]

2022 Jun 15;550:111647. PMID: 35429597
Exp Cell Res

Sparstolonin B inhibits pancreatic adenocarcinoma through the NF-κB signaling pathway. [Abstract]

2022 May 18;417(1):113214. PMID: 35594953
Mol Med Rep

Sevoflurane attenuates brain damage through inhibiting autophagy and apoptosis in cerebral ischemia‑reperfusion rats. [Abstract]

2020 Jan;21(1):123-130. PMID: 31746402
Int J Biochem Cell Biol

Resveratrol induces depletion of TRAF6 and suppresses prostate cancer cell proliferation and migration. [Abstract]

2020 Jan;118:105644. PMID: 31712163
J Steroid Biochem Mol Biol

Dihydrotestosterone regulation of cyclooxygenase-2 expression in bovine endometrial epithelium cells by androgen receptor mediated EGFR/PI3K/Akt pathway. [Abstract]

2021 Nov:214:106001. PMID: 34547381
Exp Ther Med

LncRNA XIST promotes inflammation by downregulating GRα expression in the adenoids of children with OSAHS. [Abstract]

2021 May;21(5):500. PMID: 33791009
Biochem Biophys Res Commun

Expression of CXCL13 in clear cell renal cell carcinoma: Assisting clinical diagnosis and exploring the malignant biological mechanism. [Abstract]

2025 Dec 6:795:153103. PMID: 41365162
J Environ Sci (China)

Multifunctional assays and molecular simulations reveal the protective mechanisms of QNZ in mitigating PFOA-induced cytotoxicity in Nthy-ori3-1 cells. [Abstract]

2026 Mar:161:201-208. PMID: 41461467
bioRxiv

2025 February 22.
Research Square Preprint

2025 Jan 05.
SSRN

2023 Nov 21.
Research Square Preprint

2023 May 8.
SSRN

2023 May 22.
MicroPubl Biol

Chemical Genetics Screen of EVP4593 Sensitivity in Budding Yeast Identifies Effects on Mitochondrial Structure and Function. [Abstract]

2023 Apr 25:2023:10.17912/micropub.biology.000806. PMID: 37179969
Oxid Med Cell Longev

USP7 Attenuates Endoplasmic Reticulum Stress and NF- κ B Signaling to Modulate Chondrocyte Proliferation, Apoptosis, and Inflammatory Response under Inflammation. [Abstract]

2022 Apr 6;2022:1835900. PMID: 35432716

Solvent & Solubility

In Vitro:

DMSO : ≥ 37 mg/mL (103.81 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8057 mL

14.0284 mL

28.0568 mL

5 mM

0.5611 mL

2.8057 mL

5.6114 mL

10 mM

0.2806 mL

1.4028 mL

2.8057 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (7.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[2]

iPSHD22 cells are cultured in K-4 medium in a 96-well black plates with clear flat bottom. Next, cells are treated with chemical compounds (e.g., QNZ 100 nM) for 24 h prior to analysis. Fluorescent assay MultiTox-Fluor Multiplex Cytotoxicity Assay is used to measure simultaneously the relative number of live (viability) and dead (cytotoxicity) cells in each well. Fluorescence is detected by DTX 880 Multimode Microplate Reader. To evaluate the level of cell death (LoCD), the following equation is employed: ([cytotoxicity in a well with cells]-[cytotoxicity in a well without cells])/([viability in a well with cells]-[viability in a well without cells])^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tobe M, et al. Discovery of quinazolines as a novel structural class of potent inhibitors of NF-kappa B activation. [Content Brief]

[2]. Nekrasov ED, et al. Manifestation of Huntington's disease pathology in human induced pluripotent stem cell-derived neurons. Mol Neurodegener. 2016 Apr 14;11:27. [Content Brief]

[3]. Wu J, et al. Enhanced Store-Operated Calcium Entry Leads to Striatal Synaptic Loss in a Huntington's Disease Mouse Model. J Neurosci. 2016 Jan 6;36(1):125-41. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8057 mL	14.0284 mL	28.0568 mL	70.1420 mL
	5 mM	0.5611 mL	2.8057 mL	5.6114 mL	14.0284 mL
	10 mM	0.2806 mL	1.4028 mL	2.8057 mL	7.0142 mL
	15 mM	0.1870 mL	0.9352 mL	1.8705 mL	4.6761 mL
	20 mM	0.1403 mL	0.7014 mL	1.4028 mL	3.5071 mL
	25 mM	0.1122 mL	0.5611 mL	1.1223 mL	2.8057 mL
	30 mM	0.0935 mL	0.4676 mL	0.9352 mL	2.3381 mL
	40 mM	0.0701 mL	0.3507 mL	0.7014 mL	1.7535 mL
	50 mM	0.0561 mL	0.2806 mL	0.5611 mL	1.4028 mL
	60 mM	0.0468 mL	0.2338 mL	0.4676 mL	1.1690 mL
	80 mM	0.0351 mL	0.1754 mL	0.3507 mL	0.8768 mL
	100 mM	0.0281 mL	0.1403 mL	0.2806 mL	0.7014 mL

Keywords

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QNZ

Apoptosis Compound Library

Immunology/Inflammation Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Oxygen Sensing Compound Library

Pyroptosis Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Antidepressant Compound Library

Human Metabolite Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Mitochondrial Protection Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Autoimmune Disease Compound Library

Highly Selective Inhibitors Library

Cytokine Inhibitors Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) QNZ

All NF-κB Isoforms

All TNF Receptor Isoforms

Biological Activity

Chemical Information

Publications (49)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

QNZ Related Classifications

Cat. No.: HY-13812