197 Results for "

Chromogenic Substrate

" in MedChemExpress (MCE) Product Catalog:
Products (197)

197 Results for "Chromogenic Substrate" in MCE Product Catalog:

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Referencia número: HY-123275C
No. CAS: 62354-65-8
Synonyms: S-2238 dihydrochloride
Target:  

Thrombin

Áreas de investigación:  

Others

H-D-Phe-Pip-Arg-pNA dihydrochloride (S-2238 dihydrochloride) is a specific chromogenic peptide substrate for thrombin (Thrombin). The amide bond of H-D-Phe-Pip-Arg-pNA dihydrochloride is rapidly hydrolyzed by thrombin, producing a characteristic yellow absorbance at 405 nm, and the change in absorbance is proportional to thrombin activity. H-D-Phe-Pip-Arg-pNA dihydrochloride can be used in biochemical detection-related studies .
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Referencia número: HY-P10668
No. CAS: 410532-54-6
Target:  

Dengue Virus Flavivirus

Áreas de investigación:  

Infection

Ac-EVKKQR-pNA is a competitive chromogenic para-nitroanilide substrate corresponding to the P6-P1 segment amino-terminal to the NS2B-NS3 cleavage site but with a more reactive, hydrolytically cleavable, para-nitroanilide at the P1’ position. Ac-EVKKQR-pNA is promising for research of dengue 2 virus and flavivirus virus infection .
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Referencia número: HY-W013168
No. CAS: 1492-30-4
Synonyms: 4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate; pNpp
Target:  

Lipase

Áreas de investigación:  

Others

4-Nitrophenyl palmitate (4-Nitrophenyl hexadecanoate) is a chromogenic substrate for lipases and esterases. Upon enzymatic hydrolysis, 4-Nitrophenyl palmitate releases p-nitrophenol, which can be quantified by colorimetric detection at 410 nm as a measure of enzymatic activity. 4-Nitrophenyl palmitate is used to characterize the activity of various bacterial and mammalian enzymes, including those from Burkholderia and porcine pancreatic lipase .
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Referencia número: HY-W698964
No. CAS: 60301-87-3
2′-Hydroxy-5′-nitrohexadecanamide is a lysosomal hydrolase inhibitor and is a lipid containing pentadecanoic acid and a terminal nitrophenol in its structure. 2′-Hydroxy-5′-nitrohexadecanamide can cause intracellular lipid accumulation by inhibiting lysosomal hydrolase activity. Additionally, 2′-Hydroxy-5′-nitrohexadecanamide can be used to synthesize chromogenic substrates for measuring sphingolipase activity .
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Referencia número: HY-114522
No. CAS: 107021-38-5
Pureza:  99.93%
Áreas de investigación:  

Others

X-alpha-Gal is a chromogenic substrate that is used to screen colonies with high activity of β-galactopyranoside. X-alpha-Gal also detects α-galactosidase activity. X-alpha-Gal can be used in yeast two hybrid screening for MEL1 gene activation. X-alpha-Gal can be used with Matchmaker GAL4-based products to confirm protein interactions .
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Referencia número: HY-137529
No. CAS: 21653-40-7
Pureza:  99.92%
Áreas de investigación:  

Others

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride is a chromogenic peptide substrate used for trypsin and tryptase-like serine proteases. Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride releases p-nitroaniline upon hydrolysis, which can be detected by absorbance. Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride can be used in studies of A1PI degradation .
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Referencia número: HY-D1510
No. CAS: 77640-21-2
Áreas de investigación:  

Others

4-Nitrophenyl 2-O-(β-L-Fucopyranosyl)-β-D-Galactopyranoside is a synthetic chromogenic substrate and can be used in the assay of α-fucosidases which hydrolyze the glycosidic linkage Fuc α1-2Gal. The assay is based on the sequential action of α-fucosidase and an exogenously added exo-β-d-galactosidase to release the easily measurable p-nitrophenol moiety .
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Referencia número: HY-P4582
No. CAS: 110906-89-3
Synonyms: Suc-FAAF-pNA
Target:  

Ser/Thr Protease

Áreas de investigación:  

Others

Suc-Phe-Ala-Ala-Phe-pNA (Suc-FAAF-pNA) is a chromogenic polypeptide substrate that can be used for the quantitative activity assay of intracellular subtilisin (ISP) and neutral metalloprotease (NPS) derived from Saccharomonospora canescens. Suc-Phe-Ala-Ala-Phe-pNA is hydrolyzed by the catalytic triad of mature proteases to release the chromophore pNA. Suc-Phe-Ala-Ala-Phe-pNA is applicable to enzymological studies .
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Referencia número: HY-DY2002
No. CAS: 7240-90-6
X-GAL (BCIG) (solution) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity .
Solvent and Concentration: DMF: 20 mg/mL
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Referencia número: HY-W127380
No. CAS: 146797-82-2
Arachidonoyl Thio-PC is a substrate of many phospholipase A2 (PLA2), including sPLA2, cPLA2 and iPLA2. Cleavage of sn-2 fatty acids by PLA2 results in the production of free thiols, which react with chromogenic reagents such as DTNB (Ellman's reagent) and DTP, allowing quantification of PLA2 activity. Isozyme-specific cPLA2 activity can be measured by depleting or inhibiting sPLA2 and iPLA2 activity in the assay.
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Referencia número: HY-112641
No. CAS: 1176235-08-7
AkaLumine is a D-luciferin (HY-12591A) analogue with a Km of 2.06 μM for recombinant firefly luciferase (Fluc) protein. AkaLumine emits near-infrared (NIR) light (λmax=677 nm) in reactions with native Fluc. AkaLumine has high tissue-penetration and increases detection sensitivity from deep-tissue targets .
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Referencia número: HY-146248
No. CAS: 2412923-11-4
TFMU-ADPr is a selective reporter substrate of SARS-CoV-2 Macro1 (IC50=0.59 μM), with an excitation wavelength (λEx) of 385 nm, and an emission wavelength (λEm) of 502 nm (or 495 nm). TFMU-ADPr can also undergo enzymatic hydrolysis with Poly(ADP-ribose) Glycohydrolase (PARG) sourced from human, Tetrahymena thermophila and ADP-ribosylhydrolase 3 from human to release fluorophores, thereby directly reporting total poly (ADP-ribose) hydrolase activity. TFMU-ADPr binds to the ADPr-binding site of SARS-CoV-2 Macro1, and its TFMU moiety inserts into the narrow hydrophobic groove of this protein. TFMU-ADPr can thus be used to evaluate small-molecule inhibitors targeting PAR hydrolases under in vitro conditions, to investigate the regulatory mechanisms of ADP-ribosyl catabolic enzymes, or to detect PAR hydrolase activity in whole-cell lysate assays. TFMU-ADPr is also applicable to COVID-19-related research .
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Referencia número: HY-15914
No. CAS: 54970-72-8
Synonyms: DCHBS
Target:  

Fluorescent Dye

Áreas de investigación:  

Others

DHBS (3, 5-dichloro-2-hydroxybenzenesulfonate) is used in conjunction with 4-aminoantipyrine (4-AAP) (HY-W100990) and hydrogen peroxide (H2O2) for chromogenic quantitation of peroxidase in coupled enzymatic reactions. Component of Trinder reagent for use with peroxidase to measure generation of hydrogen peroxide in automated systems .
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Referencia número: HY-15930R
No. CAS: 54827-17-7
Synonyms: BM blue (Standard); Sure Blue TMB (Standard)
TMB (Standard) is the analytical standard of TMB. This product is intended for research and analytical applications. TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922 .
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Referencia número: HY-D0144
No. CAS: 5725-89-3
Synonyms: Methoxyresorufin
Resorufin methyl ether (Methoxyresorufin) is a cytochrome P450 fluorometric substrate . Resorufin methyl ether is a relatively specific substrate for CYP1A2 activity in rodents .
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Referencia número: HY-D0829
No. CAS: 81742-10-1
3,6-Dichlorotrimellitic anhydride is the key precursor that is used for preparing a variety of dichlorinated fluoresceins and rhodamines such as TET and HEX.
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Referencia número: HY-D0994A
No. CAS: 22919-26-2
Synonyms: 4-MUP disodium; MUP disodium
4-Methylumbelliferyl phosphate (4-MUP) disodium, an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate disodium is also a nerve agent simulant .
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Referencia número: HY-D1026
No. CAS: 64664-99-9
Synonyms: 3-Carboxyumbelliferyl-β-D-galactopyranoside
CUG (3-Carboxyumbelliferyl-β-D-galactopyranoside) is a fluorogenic substrate (λex=386, λem=445 nm, ε=32K).
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Referencia número: HY-D1208
No. CAS: 160081-62-9
Alkaline phosphatase substrate is a chemiluminescent substrate for alkaline phosphatase. Alkaline phosphatase substrate can be used for enzyme-linked immunoassays .
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Referencia número: HY-D1634
No. CAS: 88899-22-3
Bz-FVR-AMC is a fluorogenic substrate for procathepsin with a kcat/Km value of 1070 mM -1s -1. The high concentration of BZ-FVR-AMC inhibits the substrate .
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