208 Results for "

cell staining

" in MedChemExpress (MCE) Product Catalog:
Products (208)

208 Results for "cell staining" in MCE Product Catalog:

209
209 Publications Verification
Cat. No.: HY-D0079
CAS No.: 104821-25-2
Synonyms: Hydroethidine; PD-MY 003
Dihydroethidium, also known as DHE, is a peroxide indicator. Dihydroethidium penetrates cell membranes to form a fluorescent protein complex with blue fluoresces. After entering the cells, Dihydroethidium is mainly localized in the cell membrane, cytoplasm and nucleus, and the staining effect is the strongest in the nucleus. Dihydroethidium produces inherent blue fluorescence with a maximum excitation wavelength of 370 nm and a maximum emission wavelength of 420 nm; after dehydrogenation, Dihydroethidium combines with RNA or DNA to produce red fluorescence with a maximum excitation wavelength of 300 nm and a maximum emission wavelength of 610 nm. 535 nm can also be used as the excitation wavelength for actual observation .
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198
198 Cited Publications
Cat. No.: HY-D0814
CAS No.: 28718-90-3
Synonyms: 4',6-Diamidino-2-phenylindole dihydrochloride
DAPI (4',6-Diamidino-2-phenylindole) dihydrochloride is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. DAPI cannot penetrate intact cell membranes and is commonly used for staining damaged and fixed cells . DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment (Ex/Em = 356/451 nm).
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198
198 Cited Publications
Cat. No.: HY-D2868
CAS No.: 47165-04-8
Synonyms: 4',6-Diamidino-2-phenylindole
DAPI (4',6-Diamidino-2-phenylindole) is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. DAPI cannot penetrate intact cell membranes and is commonly used for staining damaged and fixed cells . DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment (Ex/Em = 356/451 nm).
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192
192 Cited Publications
Cat. No.: HY-D1738
CAS No.: 28718-91-4
Synonyms: 4',6-Diamidino-2-phenylindole dilactate
DAPI (4',6-Diamidino-2-phenylindole) dilactate is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. DAPI cannot penetrate intact cell membranes and is commonly used for staining damaged and fixed cells . DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment (Ex/Em = 356/451 nm) .
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179
179 Cited Publications
Cat. No.: HY-D0815
CAS No.: 25535-16-4
Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.
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154
154 Cited Publications
Cat. No.: HY-W090090
CAS No.: 121207-31-6
Synonyms: Pyrromethene 546
BODIPY493/503 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells . Maximum excitation/emission wavelength: 493/503 nm .
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134
134 Cited Publications
Cat. No.: HY-D0938
CAS No.: 150347-59-4
Synonyms: CFSE; 5(6)-Carboxyfluorescein diacetate succinimidyl ester; 5(6)-CFDA N-succinmidyl ester
CFDA-SE is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence. After entering the cell, CFDA-SE locates in the cell membrane, cytoplasm and nucleus, and the fluorescence staining is strongest in the nucleus . CFDA-SE dye can be uniformly inherited by the cells with cell division and proliferation, and its attenuation is proportional to the number of cell divisions. This phenomenon can be detected and analyzed by flow cytometry under the excitation light of 488 nm, and can be used to detect the proliferation of cells .
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100
100 Cited Publications
Cat. No.: HY-K1073

MCE Annexin V-FITC/PI Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence (Annexin V-/PI-), early apoptosis cells show green fluorescence(Annexin V+/PI-), late apoptosis cells and necrosis cells show red and green fluorescence (Annexin V+/PI+).


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74
74 Cited Publications
Cat. No.: HY-B0324A
CAS No.: 548-62-9
Synonyms: Basic Violet 3; Gentian Violet; Methyl Violet 10B
Research Areas:  

Infection Cancer

Crystal Violet, also known as Gentian violet, methyl violet 10B, is a triphenyl-methane, an alkaline dye that binds to DNA in the nucleus of a cell, staining it a deep purple. It is often used for Gram staining to classify bacteria, or for cell or histological staining[1].
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46
46 Cited Publications
Cat. No.: HY-K1078

MCE One Step TUNEL Apoptosis Detection Kit (FITC) provides a rapid and convenient method to detect cell apoptosis. After staining cells with this kit, live cells have no fluorescence, apoptosis cells show green fluorescence.

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45
45 Cited Publications
Cat. No.: HY-118411
CAS No.: 61135-33-9
Purity:  99.78%
Synonyms: EdU
Target:  

PROTAC Linkers

Research Areas:  

Cancer

5-Ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, is incorporated into cellular DNA during DNA replication and the subsequent reaction of EdU with a fluorescent azide in a “Click” reaction. EdU staining is a fast, sensitive and reproducible method to study cell proliferation . 5-Ethynyl-2'-deoxyuridine is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs . 5-Ethynyl-2'-deoxyuridine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
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45
45 Cited Publications
Cat. No.: HY-101879
CAS No.: 65-61-2
Purity:  99.65%
Target:  

Fluorescent Dye

Research Areas:  

Others

Acridine Orange hydrochloride is a cell-permeable fluorescent nucleic acid dye and lysosomal probe with a pKa of 9.65. Acridine Orange hydrochloride enters cells via passive diffusion, is protonated and accumulates in acidic lysosomes, and forms aggregates at high concentrations, producing orange-red fluorescence; when the lysosomal proton gradient or membrane integrity is impaired, Acridine Orange hydrochloride is released into the cytoplasm, green fluorescence increases and red fluorescence decreases, so it can be used to monitor lysosomal acidification status and changes in membrane permeability. Acridine Orange hydrochloride binds to nucleic acids through intercalation or electrostatic interactions, exhibiting mainly green fluorescence when bound to double-stranded DNA and mainly red fluorescence when bound to RNA or single-stranded DNA, and can be used for nucleic acid staining and differential DNA/RNA detection. The Ex/Em of Acridine Orange hydrochloride monomer is approximately 492/525 nm; when bound to double-stranded DNA it is approximately 488/520-524 nm, and when bound to RNA or single-stranded DNA it is approximately 457/630-650 nm .
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45
45 Cited Publications
Cat. No.: HY-D0942
CAS No.: 10127-02-3
Synonyms: Euchrysine 3RX
Target:  

Fluorescent Dye

Research Areas:  

Others

Acridine Orange zinc chloride salt is a cell-permeable fluorescent nucleic acid dye and lysosomal probe with a pKa of 9.65. Acridine Orange zinc chloride salt enters cells via passive diffusion, is protonated and accumulates in acidic lysosomes, and forms aggregates at high concentrations, producing orange-red fluorescence; when the lysosomal proton gradient or membrane integrity is impaired, Acridine Orange zinc chloride salt is released into the cytoplasm, green fluorescence increases and red fluorescence decreases, so it can be used to monitor lysosomal acidification status and changes in membrane permeability. Acridine Orange zinc chloride salt binds to nucleic acids through intercalation or electrostatic interactions, exhibiting mainly green fluorescence when bound to double-stranded DNA and mainly red fluorescence when bound to RNA or single-stranded DNA, and can be used for nucleic acid staining and differential DNA/RNA detection. The Ex/Em of Acridine Orange zinc chloride salt monomer is approximately 492/525 nm; when bound to double-stranded DNA it is approximately 488/520-524 nm, and when bound to RNA or single-stranded DNA it is approximately 457/630-650 nm .
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45
45 Cited Publications
Cat. No.: HY-D0952
CAS No.: 494-38-2
Target:  

Fluorescent Dye

Research Areas:  

Others

Acridine Orange base is a cell-permeable fluorescent nucleic acid dye and lysosomal probe with a pKa of 9.65. Acridine Orange base enters cells via passive diffusion, is protonated and accumulates in acidic lysosomes, and forms aggregates at high concentrations, producing orange-red fluorescence; when the lysosomal proton gradient or membrane integrity is impaired, Acridine Orange base is released into the cytoplasm, green fluorescence increases and red fluorescence decreases, so it can be used to monitor lysosomal acidification status and changes in membrane permeability. Acridine Orange base binds to nucleic acids through intercalation or electrostatic interactions, exhibiting mainly green fluorescence when bound to double-stranded DNA and mainly red fluorescence when bound to RNA or single-stranded DNA, and can be used for nucleic acid staining and differential DNA/RNA detection. The Ex/Em of Acridine Orange base monomer is approximately 492/525 nm; when bound to double-stranded DNA it is approximately 488/520-524 nm, and when bound to RNA or single-stranded DNA it is approximately 457/630-650 nm .
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33
33 Cited Publications
Cat. No.: HY-K1071

MCE Cell Cycle and Apoptosis Analysis Kit (PI staining) provides a convenient method to detect cell cycle and cell apoptosis.


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30
30 Cited Publications
Cat. No.: HY-D1614
CAS No.: 216434-81-0
Target:  

Fluorescent Dye

Research Areas:  

Others

BODIPY493/503 methyl bromide is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells . Maximum excitation/emission wavelength: 493/503 nm .
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21
21 Cited Publications
Cat. No.: HY-D1421
CAS No.: 257277-27-3
Target:  

Fluorescent Dye

Research Areas:  

Others

PKH67 is a fluorescent cell binding dye with green fluorescence. PKH67 can stain the cell membrane and the Ex/Em is 490/502 nm. PKH67 is often used in combination with the non-specific red fluorescent dye PKH26 (Ex/Em=551/567 nm) to label cells, detect cell proliferation in vitro, and trace cells in vitro and in vivo .
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19
19 Cited Publications
Cat. No.: HY-103311
CAS No.: 11103-72-3
Purity:  ≥97.0%
Synonyms: Ammoniated ruthenium oxychloride
Target:  

Calcium Channel

Research Areas:  

Cardiovascular Disease

Ruthenium red (Ammoniated ruthenium oxychloride) is a polycationic dye widely used for electron microscopy (EM) of cells, tissues and vegetative bacteria. Ruthenium red strongly reacts with phospholipids and fatty acids and binds to acidic mucopolysaccharides. Ruthenium red is a L-type calcium current (ICa) blocker. When using Ruthenium Red to stain plant tissue sections affected by pathogens, the breakdown of mucilaginous substances by the pathogens prevents them from taking up the stain, whereas the cell walls of healthy tissue stain red .
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17
17 Cited Publications
Cat. No.: HY-K0315

MCE Hematoxylin-Eosin Staining Kit can be used for cell staining, the combination of hematoxylin and eosin results in a staining pattern where the cytoplasm appears red or pink and the nucleus takes on a blue or blue-violet.

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15
15 Cited Publications
Cat. No.: HY-K1094

MCE Viability/Cytotoxicity Assay Kit for Live & Dead Cells (Calcein/PI) enables dual fluorescent staining of both live and dead cells, making it suitable for assessing cell viability and cytotoxicity. The 500 T is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

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