SGC2085 hydrochloride
Based on 1 publication(s) in Google Scholar
SGC2085 hydrochloride is a potent and selective inhibitor of coactivator associated arginine methyltransferase 1 (CARM1) with an IC50 of 50 nM. SGC2085 hydrochloride also selectively inhibits PRMT6 with an IC50 value of 5.2 μM, but not other PRMT proteins.
For research use only. We do not sell to patients.
- CAS No.: 1821908-49-9
- Formula: C19H25ClN2O2
- Molecular Weight:348.87
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocol
- Purity & Documentation
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) SGC2085 hydrochloride
MoreAll Histone Methyltransferase Isoforms
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Biological Activity
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PRMT6 5.2 μM (IC50) |
SGC2085 hydrochloride (1 μM, 10 μM, 50 μM; 48 h) is fully selective for 21 human protein methyltransferases[1].
SGC2085 hydrochloride (10 μM; 48 h) exhibits low cell permeability and no cell activity in HEK293 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1821908-49-9
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Molecular Weight 348.87
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Formula C19H25ClN2O2
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SMILES
[H]Cl.CC1=CC(OC2=CC=C(CNC([C@@H](N)C)=O)C=C2C)=CC(C)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
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Acta Pharmacol Sin
2022 Feb;43(2):457-469. PMID: 33850273
Protocol
SGC2085 is dissolved in DMSO and diluted with appropriate medium before use. HEK293 cells are grown in 12-well plates in DMEM supplemented with 10% FBS, penicillin (100 U/mL), and streptomycin (100 μg/mL). Thirty percent confluent cells are treated with inhibitors or DMSO. After 48 h, media are removed and cells are lysed in 100 μL of total lysis buffer (20 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA, 10 mM MgCl2, 0.5% Triton X-100, 12.5 U/mL benzonase), complete EDTA-free protease inhibitor cocktail. After 3 min incubation at room temperature, SDS is added to 1% final concentration. Lysates are run on SDS-PAGE, and immunoblotting is done as outlined below to determine the levels of unmethylated and methylated BAF155[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
SGC2085 hydrochloride Related Classifications
HY-100565A Related Classifications
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