SGK1-IN-2
Based on 1 Customer Validation
SGK1-IN-2 (Compound 14h) is a selective, ATP-competitive SGK1 inhibitor, with an IC50 of 0.005 μM at 10 μM ATP and an IC50 of 0.025 μM at 500 μM ATP. SGK1-IN-2 can be used in studies of cancer, hypertension and diabetes.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 98.67%
- No. CAS: 1426214-64-3
- Fòrmula: C17H12Cl2N6O2S
- Peso molecular:435.29
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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GSK 650394 (Standard)SGK Inhibitor -
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PROTAC SGK3 degrader-1PROTAC SGK3 Degrader -
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EMD638683 (Standard)Standard of EMD638683 -
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EMD638683 (S-Form)SGK1 Inhibitor
Actividad biológica
Descripciòn
IC50 & Target
[1]|
SGK1 5-25 nM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| U2OS | IC50 |
0.63 μM
Compound: 5
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Inhibition of recombinant human SGK1 (S61 to L431, S422D) expressed in human U20S cells assessed as inhibition of GSK3beta phosphorylation after 6 hrs by fluorescence assay
Inhibition of recombinant human SGK1 (S61 to L431, S422D) expressed in human U20S cells assessed as inhibition of GSK3beta phosphorylation after 6 hrs by fluorescence assay
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[PMID: 24900601] |
In Vitro
Chemical Information
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No. CAS 1426214-64-3
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Appearance Solid
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Peso molecular 435.29
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Fòrmula C17H12Cl2N6O2S
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Color Light yellow to yellow
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SMILES
O=S(C1=CC(Cl)=CC=C1Cl)(NC2=CC=C(C3=CN=C4C(NN=C4N)=N3)C=C2)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 62.5 mg/mL (143.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.78 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.2973 mL | 11.4866 mL | 22.9732 mL | 57.4330 mL |
| 5 mM | 0.4595 mL | 2.2973 mL | 4.5946 mL | 11.4866 mL | |
| 10 mM | 0.2297 mL | 1.1487 mL | 2.2973 mL | 5.7433 mL | |
| 15 mM | 0.1532 mL | 0.7658 mL | 1.5315 mL | 3.8289 mL | |
| 20 mM | 0.1149 mL | 0.5743 mL | 1.1487 mL | 2.8716 mL | |
| 25 mM | 0.0919 mL | 0.4595 mL | 0.9189 mL | 2.2973 mL | |
| 30 mM | 0.0766 mL | 0.3829 mL | 0.7658 mL | 1.9144 mL | |
| 40 mM | 0.0574 mL | 0.2872 mL | 0.5743 mL | 1.4358 mL | |
| 50 mM | 0.0459 mL | 0.2297 mL | 0.4595 mL | 1.1487 mL | |
| 60 mM | 0.0383 mL | 0.1914 mL | 0.3829 mL | 0.9572 mL | |
| 80 mM | 0.0287 mL | 0.1436 mL | 0.2872 mL | 0.7179 mL | |
| 100 mM | 0.0230 mL | 0.1149 mL | 0.2297 mL | 0.5743 mL |
SGK1-IN-2 Related Classifications
HY-135893 Related Classifications
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