Cat. No.: HY-156382

SPOP-IN-1

Customer Review

Based on 1 Customer Validation

SPOP-IN-1 is a selective SPOP E3 ubiquitin ligase inhibitor. SPOP-IN-1 leads to the accumulation of tumor suppressors PTEN and DUSP7 and decreased levels of phosphorylated AKT and ERK in clear-cell renal cell carcinoma.

For research use only. We do not sell to patients.

Purity: 99.71%
CAS No.: 2136270-56-7
Formula: C₂₆H₃₁N₇O₂S
Molecular Weight:505.64

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

SPOP-IN-1 Chemical Structure

CAS No.: 2136270-56-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Akt Isoforms :

All ERK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A498	IC₅₀	2.1 μM Compound: 6lc	Antiproliferative activity against human A498 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human A498 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32297747]
A549	IC₅₀	16.2 μM Compound: 6lc	Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32297747]
HK-2	IC₅₀	>30 μM Compound: 6lc	Cytotoxicity against human HK2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human HK2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32297747]
Hs 766	IC₅₀	14.5 μM Compound: 6lc	Cytotoxicity against human Hs766T cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human Hs766T cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32297747]
NCI-H1299	IC₅₀	10.7 μM Compound: 6lc	Cytotoxicity against human H1299 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human H1299 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32297747]
OS-RC-2	IC₅₀	3.5 μM Compound: 6lc	Antiproliferative activity against human OS-RC2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human OS-RC2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32297747]
U-251	IC₅₀	10.2 μM Compound: 6lc	Cytotoxicity against human U251 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human U251 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32297747]

Chemical Information

CAS No. 2136270-56-7
Appearance Solid
Molecular Weight 505.64
Formula C₂₆H₃₁N₇O₂S
Color Light yellow to yellow
SMILES

O=C(C1=CC2=C(N(CCC3=CC=CS3)C1=N)N=C4C(C)=CC=CN4C2=O)NCCN5CCN(CC5)C
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (9.89 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9777 mL

9.8885 mL

19.7769 mL

5 mM

0.3955 mL

1.9777 mL

3.9554 mL

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Dong Z, et al. Structure-Activity Relationship of SPOP Inhibitors against Kidney Cancer. J Med Chem. 2020 May 14;63(9):4849-4866. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9777 mL	9.8885 mL	19.7769 mL	49.4423 mL
DMSO	5 mM	0.3955 mL	1.9777 mL	3.9554 mL	9.8885 mL

Keywords

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SPOP-IN-1

Immunology/Inflammation Compound Library

MAPK Compound Library

Metabolism/Protease Compound Library

PI3K/Akt/mTOR Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Oxygen Sensing Compound Library

Ubiquitination Compound Library

Glycolysis Compound Library

Cytoskeleton Compound Library

Glutamine Metabolism Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Angiogenesis-Related Compound Library

Glucose Metabolism Compound Library

Anti-Liver Cancer Compound Library

Pain-Related Compound Library

Metabolic Enzyme Compound Library

Highly Selective Inhibitors Library

Multi-Target Compound Library

Mitophagy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Amino Acid Metabolism Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Lactylation Compound Library

All Akt Isoforms

All ERK Isoforms