B-Raf IN 19
B-Raf IN 19 (compound 31) is a BRAF inhibitor that inhibits BRAF(WT) and BRAF(V600E) (IC50: 0.57 μM and 0.28 μM, respectively). B-Raf IN 19 inhibits MAPK signaling in melanoma cells.
For research use only. We do not sell to patients.
- CAS No.: 708218-62-6
- Formula: C20H15NO3S3
- Molecular Weight:413.53
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
IC50 & Target
[1]|
BRAFWT 0.57 μM (IC50) |
BRafV600E 0.28 μM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Sf9 | IC50 |
0.28 μM
Compound: 31
|
Inhibition of human 6x-His tagged BRAF V600E mutant expressed in Sf9 cells by ELISA based kinase assay
Inhibition of human 6x-His tagged BRAF V600E mutant expressed in Sf9 cells by ELISA based kinase assay
|
[PMID: 22537109] |
| Sf9 | IC50 |
0.57 μM
Compound: 31
|
Inhibition of human 6x-His tagged wild type BRAF expressed in Sf9 cells by ELISA based kinase assay
Inhibition of human 6x-His tagged wild type BRAF expressed in Sf9 cells by ELISA based kinase assay
|
[PMID: 22537109] |
Chemical Information
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CAS No. 708218-62-6
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Molecular Weight 413.53
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Formula C20H15NO3S3
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SMILES
O=S(NC1=CC(SC2=CC=CC=C2)=C(C3=C1C=CC=C3)O)(C4=CC=CS4)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)