Thalidomide 4-chloro

Cat. No.: HY-W460141 Purity: 99.74%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Thalidomide 4-chloro is a chlorinated E3 ligase activator. Thalidomide 4-chloro may be used for further derivitization by substitution of chlorine.

For research use only. We do not sell to patients.

Thalidomide 4-chloro Chemical Structure

CAS No. : 244057-36-1

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

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•Related Screening Libraries:

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View All Ligands for E3 Ligase Isoform Specific Products:

View All Isoforms

von Hippel-Lindau (VHL) MDM2 Cereblon IAP KLHDC2 Fbxo3 RNF4 DCAF11 DCAF1 UHRF1 TRIM21 NEURL1B KLHL41

Biological Activity
Purity & Documentation
Customer Review

Description

Thalidomide 4-chloro is a chlorinated E3 ligase activator. Thalidomide 4-chloro may be used for further derivitization by substitution of chlorine.

Cellular Effect

Cell Line	Type	Value	Description	References
NAMALVA	IC₅₀	> 1 μM Compound: 26	Antiproliferative activity against human NAMALWA cells assessed as inhibition of [3H]thymidine incorporation after 72 hrs by scintillation counting Antiproliferative activity against human NAMALWA cells assessed as inhibition of [3H]thymidine incorporation after 72 hrs by scintillation counting	[PMID: 23168019]
PBMC	IC₅₀	> 1 μM Compound: 26	Inhibition of TNF-alpha production in LPS-stimulated human PBMC preincubated for 1 hr before LPS challenge measured after 28 to 20 hrs by ELISA Inhibition of TNF-alpha production in LPS-stimulated human PBMC preincubated for 1 hr before LPS challenge measured after 28 to 20 hrs by ELISA	[PMID: 23168019]
T-cell	EC₅₀	> 1 μM Compound: 26	Inhibition of IL-2 production in human T cells measured after 2 to 3 days by ELISA Inhibition of IL-2 production in human T cells measured after 2 to 3 days by ELISA	[PMID: 23168019]

Molecular Weight

292.68

Formula

C₁₃H₉ClN₂O₄

CAS No.

244057-36-1

Appearance

Solid

Color

Brown to gray

SMILES

O=C1N(C(CC2)C(NC2=O)=O)C(C3=C1C=CC=C3Cl)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (113.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4167 mL	17.0835 mL	34.1670 mL
5 mM	0.6833 mL	3.4167 mL	6.8334 mL
10 mM	0.3417 mL	1.7084 mL	3.4167 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 3.33 mg/mL (11.38 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 3.33 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (33.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3.33 mg/mL (11.38 mM); Clear solution

This protocol yields a clear solution of ≥ 3.33 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (33.3 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.74%

Select Batch:

Data Sheet (246 KB) SDS (394 KB)

COA (252 KB) HNMR (114 KB) RP-HPLC (245 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4167 mL	17.0835 mL	34.1670 mL	85.4175 mL
	5 mM	0.6833 mL	3.4167 mL	6.8334 mL	17.0835 mL
	10 mM	0.3417 mL	1.7084 mL	3.4167 mL	8.5418 mL
	15 mM	0.2278 mL	1.1389 mL	2.2778 mL	5.6945 mL
	20 mM	0.1708 mL	0.8542 mL	1.7084 mL	4.2709 mL
	25 mM	0.1367 mL	0.6833 mL	1.3667 mL	3.4167 mL
	30 mM	0.1139 mL	0.5695 mL	1.1389 mL	2.8473 mL
	40 mM	0.0854 mL	0.4271 mL	0.8542 mL	2.1354 mL
	50 mM	0.0683 mL	0.3417 mL	0.6833 mL	1.7084 mL
	60 mM	0.0569 mL	0.2847 mL	0.5695 mL	1.4236 mL
	80 mM	0.0427 mL	0.2135 mL	0.4271 mL	1.0677 mL
	100 mM	0.0342 mL	0.1708 mL	0.3417 mL	0.8542 mL