Thalidomide 4-chloro
Based on 1 Customer Validation
Thalidomide 4-chloro is a chlorinated E3 ligase activator. Thalidomide 4-chloro may be used for further derivitization by substitution of chlorine.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 244057-36-1
- Formula: C13H9ClN2O4
- Molecular Weight:292.68
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
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Help & FAQs
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Ubiquitination Compound Library
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E3 Ligase Ligand Library
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Bioactive Compound Library Max
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Bioactive Compound Library
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High-Throughput Bioactive Compound Library
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3D Diverse Fragment Library
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MCE Kinase Hinge Binder Fragment Library
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Molecular Glue-like Compound Library
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Anti-Cancer Compound Library
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| NAMALVA | IC50 |
>1 μM
Compound: 26
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Antiproliferative activity against human NAMALWA cells assessed as inhibition of [3H]thymidine incorporation after 72 hrs by scintillation counting
Antiproliferative activity against human NAMALWA cells assessed as inhibition of [3H]thymidine incorporation after 72 hrs by scintillation counting
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[PMID: 23168019] |
| PBMC | IC50 |
>1 μM
Compound: 26
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Inhibition of TNF-alpha production in LPS-stimulated human PBMC preincubated for 1 hr before LPS challenge measured after 28 to 20 hrs by ELISA
Inhibition of TNF-alpha production in LPS-stimulated human PBMC preincubated for 1 hr before LPS challenge measured after 28 to 20 hrs by ELISA
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[PMID: 23168019] |
| T-cell | EC50 |
>1 μM
Compound: 26
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Inhibition of IL-2 production in human T cells measured after 2 to 3 days by ELISA
Inhibition of IL-2 production in human T cells measured after 2 to 3 days by ELISA
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[PMID: 23168019] |
Chemical Information
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CAS No. 244057-36-1
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Appearance Solid
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Molecular Weight 292.68
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Formula C13H9ClN2O4
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Color Brown to gray
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SMILES
O=C1N(C(CC2)C(NC2=O)=O)C(C3=C1C=CC=C3Cl)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 33.33 mg/mL (113.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 3.33 mg/mL (11.38 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 3.33 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (33.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (246 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4167 mL | 17.0835 mL | 34.1670 mL | 85.4175 mL |
| 5 mM | 0.6833 mL | 3.4167 mL | 6.8334 mL | 17.0835 mL | |
| 10 mM | 0.3417 mL | 1.7084 mL | 3.4167 mL | 8.5418 mL | |
| 15 mM | 0.2278 mL | 1.1389 mL | 2.2778 mL | 5.6945 mL | |
| 20 mM | 0.1708 mL | 0.8542 mL | 1.7084 mL | 4.2709 mL | |
| 25 mM | 0.1367 mL | 0.6833 mL | 1.3667 mL | 3.4167 mL | |
| 30 mM | 0.1139 mL | 0.5695 mL | 1.1389 mL | 2.8473 mL | |
| 40 mM | 0.0854 mL | 0.4271 mL | 0.8542 mL | 2.1354 mL | |
| 50 mM | 0.0683 mL | 0.3417 mL | 0.6833 mL | 1.7084 mL | |
| 60 mM | 0.0569 mL | 0.2847 mL | 0.5695 mL | 1.4236 mL | |
| 80 mM | 0.0427 mL | 0.2135 mL | 0.4271 mL | 1.0677 mL | |
| 100 mM | 0.0342 mL | 0.1708 mL | 0.3417 mL | 0.8542 mL |
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