USP28-IN-2
USP28-IN-2 is a USP28 inhibitor (IC50=0.3 μM) with high selectivity over USP2, USP7, USP8, USP9x, UCHL3 and UCHL5. USP28-IN-2 shows cytotoxicity against cancer cells, down-regulates the cellular level of c-Myc through ubiquitin-proteasome system. USP28-IN-2 also decreases the ankyrase-1/2 level in vitro. USP28-IN-2 enhance the sensitivity of colorectal cancer cells to Regorafenib (HY-10331).
For research use only. We do not sell to patients.
- CAS No.: 2931509-11-2
- Formula: C23H20Cl2N2O3S
- Molecular Weight:475.39
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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USP28 0.3 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
26.01 μM
Compound: 9l
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Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition incubated for 3 days by SRB assay
Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition incubated for 3 days by SRB assay
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[PMID: 37075624] |
| LS174T | IC50 |
21.9 μM
Compound: 9l
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Antiproliferative activity against human LS174T cells assessed as cell growth inhibition incubated for 3 days by SRB assay
Antiproliferative activity against human LS174T cells assessed as cell growth inhibition incubated for 3 days by SRB assay
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[PMID: 37075624] |
USP28-IN-2 (compound 9l) (15 μM, 17.5 μM; 3 d) inhibits colony formation of human colorectal cancer cells HCT116 (17.5 μM) and Ls174T (15 μM)[1].
USP28-IN-2 (20-80 μM; 24 h) down-regulates the level of c-Myc by enhancing its degradation via ubiquitin-proteasome system (UPS)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human colorectal cancer cells HCT116 and Ls174T
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Concentration:20 μM, 30 μM, 50 μM, and 60 μM, for Ls174T; 30 μM, 50 μM, 60 μM and 80 μM for HCT116
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Incubation Time:24 h
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Result:Dose-dependently down-regulated the cellular level of c-Myc.
Chemical Information
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CAS No. 2931509-11-2
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Molecular Weight 475.39
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Formula C23H20Cl2N2O3S
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SMILES
CS(=O)(C1=CC=C(C(Cl)=C1)C(NC2=CC=C(C(C3=CC=C(C=C3)C4(CC4)N)=C2)Cl)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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