1. Cell Cycle/DNA Damage
  2. Deubiquitinase
  3. USP28-IN-2

USP28-IN-2 is a USP28 inhibitor (IC50=0.3 μM) with high selectivity over USP2, USP7, USP8, USP9x, UCHL3 and UCHL5. USP28-IN-2 shows cytotoxicity against cancer cells, down-regulates the cellular level of c-Myc through ubiquitin-proteasome system. USP28-IN-2 also decreases the ankyrase-1/2 level in vitro. USP28-IN-2 enhance the sensitivity of colorectal cancer cells to Regorafenib (HY-10331).

For research use only. We do not sell to patients.

USP28-IN-2 Chemical Structure

USP28-IN-2 Chemical Structure

CAS No. : 2931509-11-2

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Description

USP28-IN-2 is a USP28 inhibitor (IC50=0.3 μM) with high selectivity over USP2, USP7, USP8, USP9x, UCHL3 and UCHL5. USP28-IN-2 shows cytotoxicity against cancer cells, down-regulates the cellular level of c-Myc through ubiquitin-proteasome system. USP28-IN-2 also decreases the ankyrase-1/2 level in vitro. USP28-IN-2 enhance the sensitivity of colorectal cancer cells to Regorafenib (HY-10331)[1].

IC50 & Target[1]

USP28

0.3 μM (IC50)

In Vitro

USP28-IN-2 (compound 9l) (15 μM, 17.5 μM; 3 d) inhibits colony formation of human colorectal cancer cells HCT116 (17.5 μM) and Ls174T (15 μM)[1].
USP28-IN-2 (20-80 μM; 24 h) down-regulates the level of c-Myc by enhancing its degradation via ubiquitin-proteasome system (UPS)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: Human colorectal cancer cells HCT116 and Ls174T
Concentration: 20 μM, 30 μM, 50 μM, and 60 μM, for Ls174T; 30 μM, 50 μM, 60 μM and 80 μM for HCT116
Incubation Time: 24 h
Result: Dose-dependently down-regulated the cellular level of c-Myc.
Molecular Weight

475.39

Formula

C23H20Cl2N2O3S

CAS No.
SMILES

CS(=O)(C1=CC=C(C(Cl)=C1)C(NC2=CC=C(C(C3=CC=C(C=C3)C4(CC4)N)=C2)Cl)=O)=O

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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USP28-IN-2 Related Classifications

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Product Name:
USP28-IN-2
Cat. No.:
HY-149228
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