2. Metabolic Enzyme/Protease Cytoskeleton Apoptosis Membrane Transporter/Ion Channel
  3. Phosphodiesterase (PDE) Myosin Apoptosis Calmodulin Potassium Channel
  4. W-7 hydrochloride

W-7 (hydrochloride)  (Synonyms: W-7塩酸塩)

製品番号: HY-100912 純度: 99.96%
COA 取扱説明書 Technical Support

W-7 hydrochloride is a selective calmodulin antagonist. W-7 hydrochloride inhibits the Ca2+-calmodulin-dependent phosphodiesterase and myosin light chain kinase with IC50 values of 28 μM and 51 μM, respectively. W-7 hydrochloride induces apoptosis and has antitumor and vascular relaxing activity. W-7 hydrochloride is a blocker of Kv4.3 and can be used for research of arrhythmias.

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CAS 番号 : 61714-27-0

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>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 29 在庫あり
Solution
10 mM * 1 mL in DMSO USD 29 在庫あり
Solid
5 mg $27 在庫あり
10 mg $40 在庫あり
25 mg $65 在庫あり
50 mg $90 在庫あり
100 mg $135 在庫あり
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500 mg   お問い合わせ  

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カスタマーレビュー

Based on 7 publication(s) in Google Scholar

Other Forms of W-7 hydrochloride:

W-7 (hydrochloride) 関連抗体

Top Publications Citing Use of Products

    W-7 hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2025 Oct 29:S1097-2765(25)00822-6.  [Abstract]

    HEK293T cells expressing FLAG‑tagged RNF32 were treated with PMA and IO for 90 min, then immunoprecipitated (IP) with an anti‑RNF32 antibody in the presence or absence of the indicated CaM inhibitors W-7 hydrochloride (25-100 μM) or Calmidazolium. Whole cell extracts (WCEs) were immunoprecipitated with anti-FLAG resin. Immunocomplexes and WCEs were analyzed by immunoblotting. The results showed that the CaM inhibitors W-7 hydrochloride and Calmidazolium reduced the binding of RNF32 to the IKK complex in cells treated with PMA and IO.

    W-7 hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2025 Oct 29:S1097-2765(25)00822-6.  [Abstract]

    Immunofluorescence staining of endogenous RNF32 in U2OS cells stimulated with 10 ng/ml phorbol-12-myristate-13-acetate (PMA) and 950 nM ionomycin (IO) for 90 minutes with or without the CaM inhibitor W‑7 hydrochloride (25-100 μM). The results showed that the CaM inhibitors W‑7 hydrochloride greatly reduced the formation of RNF32 condensates. Scale bars indicate 10 µm.

    W-7 hydrochloride purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Dec 31;39(24):e71322.  [Abstract]

    Results of CCK-8 assays assessing the proliferation of OSCC cells (Cal-27, HN4) in the sgNC and sgORAI3 groups, as well as following treatment with inhibitors W-7 hydrochloride (10 μM) for calmodulin and CsA for calcineurin. Fold changes are relative to the optical density at a wavelength of 450 nm at time 0 h.

    W-7 hydrochloride purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Dec 31;39(24):e71322.  [Abstract]

    Representative images of scratch assays showing the migration of OSCC cells in the sgNC and sgORAI3 groups, as well as following treatment with W-7 hydrochloride (10 μM; 24-48 h) and CsA. Scale bar, 200 μm.

    W-7 hydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Aug 3:134478.  [Abstract]

    The relative expression levels of CaM and CN genes following inhibitor treatment at 48 h. The results showed that 10 μM W-7 hydrochloride (48 h) significantly downregulated CaM genes in both insects, while FK506 exhibited superior efficacy at 100 μM.

    W-7 hydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Aug 3:134478.  [Abstract]

    The survival rate of G. molesta and C. chinensis after treatment with inhibitors and cyantraniliprole (CY). The results showed that injection or oral administration of W-7 hydrochloride (10 μM) or FK506 (100 μM) increased the sensitivity of both G. molesta and C. chinensis to cyantraniliprole (LC20), resulting in a notable rise in mortality rates, with the cyantraniliprole/W7 group showing the highest mortality.

    Phosphodiesterase (PDE) アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

    Myosin アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    MLCK

    Calmodulin アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    CALM1 CALM2 CALM3

    • 生物活性

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    製品説明

    W-7 hydrochloride is a selective calmodulin antagonist. W-7 hydrochloride inhibits the Ca2+-calmodulin-dependent phosphodiesterase and myosin light chain kinase with IC50 values of 28 μM and 51 μM, respectively. W-7 hydrochloride induces apoptosis and has antitumor and vascular relaxing activity. W-7 hydrochloride is a blocker of Kv4.3 and can be used for research of arrhythmias[1][2][3][4][5][6].

    IC50 & Target

    IC50: 28 μM (Phosphodiesterase) and 51 µM (Myosin light chain kinase)[1]
    体外実験

    W-7 hydrochloride is distributed mainly in the cytoplasm, and inhibits proliferation of Chinese hamster ovary K1 (CHO-K1) cells. W-7 hydrochloride selectively blocks the phase of the cell cycle (G1/S boundary phase) in a manner. 25 μM W-7 hydrochloride arrests the growth of the cells at the G1/S boundary phase of the cell cycle[1].
    W-7 (100 μM) hydrochloride exhibits a similar extent of antagonism between the contractile responses to carbachol and KCl. The increase in myosin light chain (P-LC) phosphate content in response to 1-min stimulation with 10 μM carbachol is inhibited by W-7 hydrochloride. W-7 hydrochloride antagonizes the smooth muscle contraction through the inhibition of the initial increase in the P-LC phosphorylation[2].
    Treatment with W-7 hydrochloride results in the dose-dependent inhibition of cell proliferation in various human multiple myeloma cell lines. W-7 hydrochloride induces G1 phase cell cycle arrest by downregulating cyclins and upregulating p21cip1. W-7 hydrochloride induces apoptosis via caspase activation; this occurred partly through the elevation of intracellular calcium levels and mitochondrial membrane potential depolarization and through inhibition of the STAT3 phosphorylation and subsequent downregulation of Mcl-1 protein[3].
    W-7 hydrochloride competitively inhibits Ca2+/calmodulin-dependent phosphodiesterase with a Ki value of 300 μM[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    W-7 (hydrochloride) 関連抗体
    体内実験

    W-7 (3 mg/kg; intraperitoneal injection; on 5 consecutive days per week; female BALB/c nu mice) hydrochloride treatment significantly reduces tumor growth in a murine MM model[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female BALB/c nu mice (6-week-old) injected with RPMI 8226 cells[3]
    Dosage: 3 mg/kg
    Administration: Intraperitoneal injection; on 5 consecutive days per week
    Result: Significantly reduced tumor growth in a murine MM model.
    分子量

    377.33

    分子式

    C16H22Cl2N2O2S
    CAS 番号
    Appearance

    Solid

    Color

    Off-white to yellow

    SMILES

    O=S(C1=C2C=CC=C(Cl)C2=CC=C1)(NCCCCCCN)=O.[H]Cl
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    DMSO : 250 mg/mL (662.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 1.43 mg/mL (3.79 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6502 mL 13.2510 mL 26.5020 mL
    5 mM 0.5300 mL 2.6502 mL 5.3004 mL
    10 mM 0.2650 mL 1.3251 mL 2.6502 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

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    体積 （開始）

    V1

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    濃度 （終了）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (5.51 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (5.51 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    g

    Dosing volume
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    μL

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    % 
    +
    %
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.96%

    COA (246 KB) LCMS (83 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.6502 mL 13.2510 mL 26.5020 mL 66.2550 mL
    DMSO 5 mM 0.5300 mL 2.6502 mL 5.3004 mL 13.2510 mL
    10 mM 0.2650 mL 1.3251 mL 2.6502 mL 6.6255 mL
    15 mM 0.1767 mL 0.8834 mL 1.7668 mL 4.4170 mL
    20 mM 0.1325 mL 0.6626 mL 1.3251 mL 3.3128 mL
    25 mM 0.1060 mL 0.5300 mL 1.0601 mL 2.6502 mL
    30 mM 0.0883 mL 0.4417 mL 0.8834 mL 2.2085 mL
    40 mM 0.0663 mL 0.3313 mL 0.6626 mL 1.6564 mL
    50 mM 0.0530 mL 0.2650 mL 0.5300 mL 1.3251 mL
    60 mM 0.0442 mL 0.2209 mL 0.4417 mL 1.1043 mL
    80 mM 0.0331 mL 0.1656 mL 0.3313 mL 0.8282 mL
    100 mM 0.0265 mL 0.1325 mL 0.2650 mL 0.6626 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    W-7 hydrochloride Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    W-761714-27-0W7W 7Phosphodiesterase (PDE)MyosinApoptosisCalmodulinPotassium ChannelCaMCalcium-Modulated ProteinKcsACa2+calmodulinmyosinlightchainphosphateanti-proliferationphosphodiesteraseantitumorInhibitorinhibitorinhibit

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    製品名:
    W-7 hydrochloride
    製品番号:
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