Cat. No.: HY-133180

YW1128

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YW1128 (compound 3a) is a potent Wnt/β-Catenin inhibitor. YW1128 induces the proteasome degradation of β-catenin and subsequent inhibits the Wnt/β-catenin signaling in cells. YW1128 significantly decreases hepatic lipid accumulation. YW1128 improves glucose tolerance of high fat diet-fed mice without noticeable toxicity. YW1128 down regulates the genes involved in the glucose and fatty acid anabolism.

For research use only. We do not sell to patients.

Purity: 99.38%
CAS No.: 2131223-64-6
Formula: C₂₀H₁₇N₅O
Molecular Weight:343.38

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

YW1128 Chemical Structure

CAS No.: 2131223-64-6

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Sample (0.1mg)

In-stock

(In Japan)

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All Wnt Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	20 nM Compound: 3a	Inhibition of Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring siRNA after 24 hrs by luciferase reporter gene assay Inhibition of Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring siRNA after 24 hrs by luciferase reporter gene assay	[PMID: 30605343]
HEK293	IC₅₀	34 nM Compound: 3a	Inhibition of Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring GSK3B siRNA after 24 hrs by luciferase reporter gene assay Inhibition of Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring GSK3B siRNA after 24 hrs by luciferase reporter gene assay	[PMID: 30605343]
HEK293	IC₅₀	4.1 nM Compound: 3a	Inhibition of Wnt3a activated Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring TCF/LEF-responsive luciferase preincubated with cells followed by Wnt3a addition and measured after 24 hrs by luciferase reporter gene assay Inhibition of Wnt3a activated Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring TCF/LEF-responsive luciferase preincubated with cells followed by Wnt3a addition and measured after 24 hrs by luciferase reporter gene assay	[PMID: 30605343]
HEK293	IC₅₀	6 nM Compound: 3a	Inhibition of LiCl activated Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring TCF/LEF-responsive luciferase preincubated with cells followed by LiCl addition and measured after 24 hrs by luciferase reporter gene assay Inhibition of LiCl activated Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring TCF/LEF-responsive luciferase preincubated with cells followed by LiCl addition and measured after 24 hrs by luciferase reporter gene assay	[PMID: 30605343]
K562	IC₅₀	>50 μM Compound: 1	Anti-leukemia activity in human K562 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay Anti-leukemia activity in human K562 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay	[PMID: 35777269]

Chemical Information

CAS No. 2131223-64-6
Appearance Solid
Molecular Weight 343.38
Formula C₂₀H₁₇N₅O
Color White to off-white
SMILES

O=C(C1=C(C)N(C2=CC=CC=C2C)N=N1)NC3=NC4=CC=CC=C4C=C3
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (291.22 mM; ultrasonic and warming and heat to 160°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9122 mL

14.5611 mL

29.1223 mL

5 mM

0.5824 mL

2.9122 mL

5.8245 mL

10 mM

0.2912 mL

1.4561 mL

2.9122 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 2.5 mg/mL (7.28 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Obianom ON, et al. Triazole-Based Inhibitors of the Wnt/β-Catenin Signaling Pathway Improve Glucose and Lipid Metabolisms in Diet-Induced Obese Mice. J Med Chem. 2019 Jan 24;62(2):727-741. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9122 mL	14.5611 mL	29.1223 mL	72.8056 mL
	5 mM	0.5824 mL	2.9122 mL	5.8245 mL	14.5611 mL
	10 mM	0.2912 mL	1.4561 mL	2.9122 mL	7.2806 mL
	15 mM	0.1941 mL	0.9707 mL	1.9415 mL	4.8537 mL
	20 mM	0.1456 mL	0.7281 mL	1.4561 mL	3.6403 mL
	25 mM	0.1165 mL	0.5824 mL	1.1649 mL	2.9122 mL
	30 mM	0.0971 mL	0.4854 mL	0.9707 mL	2.4269 mL
	40 mM	0.0728 mL	0.3640 mL	0.7281 mL	1.8201 mL
	50 mM	0.0582 mL	0.2912 mL	0.5824 mL	1.4561 mL
	60 mM	0.0485 mL	0.2427 mL	0.4854 mL	1.2134 mL
	80 mM	0.0364 mL	0.1820 mL	0.3640 mL	0.9101 mL
	100 mM	0.0291 mL	0.1456 mL	0.2912 mL	0.7281 mL

Keywords

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YW1128

Stem Cell Signaling Compound Library

Wnt/Hedgehog/Notch Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Cytoskeleton Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Antihypertensive Compound Library

Extracellular Vesicles (EVs) Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

Cell Proliferation Compound Library

Lactic Acid Metabolic Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

All Wnt Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

YW1128

Stem Cell Signaling Compound Library

Wnt/Hedgehog/Notch Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Cytoskeleton Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Antihypertensive Compound Library

Extracellular Vesicles (EVs) Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

Cell Proliferation Compound Library

Lactic Acid Metabolic Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

All Wnt Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

YW1128 Related Classifications

Cat. No.: HY-133180