2. Cell Cycle/DNA Damage Antibody-drug Conjugate/ADC Related
  3. DNA/RNA Synthesis ADC Payload
  4. β-Amanitin

β-Amanitin is a cyclic peptide toxin in the poisonous Amanita phalloides mushroom. β-Amanitin inhibits inhibits eukaryotic RNA polymerase II and III. β-Amanitin inhibits protein synthesis. β-Amanitin can be used as a cytotoxic component of antibody-drug conjugates (ADCs).

For research use only. We do not sell to patients.

Custom Peptide Synthesis

β-Amanitin

β-Amanitin Chemical Structure

CAS No. : 21150-22-1

Size Price Stock Quantity
100 μg In-stock
500 μg In-stock
1 mg In-stock
5 mg   Get quote  
10 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 Customer Validation

β-Amanitin Related Antibodies

Top Publications Citing Use of Products

View All DNA/RNA Synthesis Isoform Specific Products:

View All Isoforms
RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

View All ADC Payload Isoform Specific Products:

View All Isoforms
Auristatin Calicheamicins Camptothecins Daunorubicins/Doxorubicins Duocarmycins Maytansinoids Pyrrolobenzodiazepines Traditional Cytotoxic Agents

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

β-Amanitin is a cyclic peptide toxin in the poisonous Amanita phalloides mushroom. β-Amanitin inhibits inhibits eukaryotic RNA polymerase II and III. β-Amanitin inhibits protein synthesis. β-Amanitin can be used as a cytotoxic component of antibody-drug conjugates (ADCs)[1][2].

IC50 & Target[1]

RNA Polymerase

 

Traditional Cytotoxic Agents

 

In Vitro

β-Amanitin (0.01-100 μg/mL; 36 hours) shows toxicity in MCF-7 cells, and the rates of cell viability are calculated as 52%, 62%, 84%, 86%, and 91% at concentrations of 100, 10, 1, 0.1, and 0.01 μg/mL, respectively[2].
β-Amanitin shows a great inhibition of protein synthesis at both concentrations (10 μg/mL and 1 μg/mL) in MCF-7 cells for 24 hours[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

β-Amanitin Related Antibodies

Cell Viability Assay[2]

Cell Line: MCF-7 cells
Concentration: 0.01, 0.1, 1, 10, 100 µg/mL
Incubation Time: 36 hours
Result: Showed toxicity in MCF-7 cells.
Molecular Weight

919.95

Formula

C39H53N9O15S
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Ile-{6-hydroxy-Trp}-Gly-Ile-Gly-Cys-Asn-Pro (Sulfidebridge try4-cys8)
Sequence Shortening

D-{6-hydroxy-Trp}-IWGIGC (Sulfidebridge try4-cys8)
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 20 mg/mL (21.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 10 mg/mL (10.87 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0870 mL 5.4351 mL 10.8702 mL
5 mM 0.2174 mL 1.0870 mL 2.1740 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.02%

SDS (480 KB)

COA (257 KB) RP-HPLC (232 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.0870 mL 5.4351 mL 10.8702 mL 27.1754 mL
5 mM 0.2174 mL 1.0870 mL 2.1740 mL 5.4351 mL
10 mM 0.1087 mL 0.5435 mL 1.0870 mL 2.7175 mL
DMSO 15 mM 0.0725 mL 0.3623 mL 0.7247 mL 1.8117 mL
20 mM 0.0544 mL 0.2718 mL 0.5435 mL 1.3588 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

β-Amanitin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

β-Amanitin21150-22-1DNA/RNA SynthesisADC PayloadADC CytotoxincyclicpeptidetoxinpoisonousmushroomcytotoxicproteinsynthesisInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
β-Amanitin
Cat. No.:
HY-125586
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (480 KB) Français - FR (480 KB) Deutsch - DE (480 KB) Norwegian - NO (480 KB) Español - ES (480 KB) Swedish - SV (480 KB) Italian - IT (480 KB) Korean - KR (480 KB) Portuguese - PT (480 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.