Cat. No.: HY-136341

7,8-Dihydroneopterin

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7,8-Dihydroneopterin, an inflammation marker, induces cellular apoptosis in astrocytes and neurons via enhancement of nitric oxide synthase (iNOS) expression. 7,8-Dihydroneopterin can be used in the research of neurodegenerative diseases.

For research use only. We do not sell to patients.

Purity: 97.2%
CAS No.: 1218-98-0
Formula: C₉H₁₃N₅O₄
Molecular Weight:255.23

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

7,8-Dihydroneopterin Chemical Structure

CAS No.: 1218-98-0

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

iNOS	Human Endogenous Metabolite

All NO Synthase Isoforms :

All Endogenous Metabolite Isoforms :

In Vitro

7,8-Dihydroneopterin is a heterocyclic pteridine which produced and secreted by monocytes/macrophages upon stimulation with the cytokine interferon-y (IFN-y)^[1].
7,8-Dihydroneopterin (0.1, 0.5, 1, 2.5, 5 mM; 2 days and 5 days) inhibits growth of U373MG astroeytes^[1] .

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Astrocytic cell line U373MG
Concentration:

0.1, 0.5, 1, 2.5, 5 mM
Incubation Time:

2 days and 5 days
Result:

After 2 days of treatment, a significantly lower cell number was observed for those cells treated with 5 mM and 2.5 mM.
After incubation for 5 days concentrations of 1-5 mM completely abrogated acid phosphatase activity to background level found for complete cell culture medium.
A significant cell growth inhibition was also visible for the cells incubated with 0.5 mM, whereas 0.1 mM did not alter cell growth.

Chemical Information

CAS No. 1218-98-0
Appearance Solid
Molecular Weight 255.23
Formula C₉H₁₃N₅O₄
Color Light yellow to yellow
SMILES

O=C1NC(N)=NC2=C1N=C([C@H](O)[C@H](O)CO)CN2
Structure Classification
- Others
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (979.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.9180 mL

19.5902 mL

39.1803 mL

5 mM

0.7836 mL

3.9180 mL

7.8361 mL

10 mM

0.3918 mL

1.9590 mL

3.9180 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 6.25 mg/mL (24.49 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (8.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (8.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. C Speth, et al. Inflammation Marker 7,8-dihydroneopterin Induces Apoptosis of Neurons and Glial Cells: A Potential Contribution to Neurodegenerative Processes. Immunobiology. 2000 Nov;202(5):460-76. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.9180 mL	19.5902 mL	39.1803 mL	97.9509 mL
	5 mM	0.7836 mL	3.9180 mL	7.8361 mL	19.5902 mL
	10 mM	0.3918 mL	1.9590 mL	3.9180 mL	9.7951 mL
	15 mM	0.2612 mL	1.3060 mL	2.6120 mL	6.5301 mL
	20 mM	0.1959 mL	0.9795 mL	1.9590 mL	4.8975 mL
	25 mM	0.1567 mL	0.7836 mL	1.5672 mL	3.9180 mL
	30 mM	0.1306 mL	0.6530 mL	1.3060 mL	3.2650 mL
	40 mM	0.0980 mL	0.4898 mL	0.9795 mL	2.4488 mL
	50 mM	0.0784 mL	0.3918 mL	0.7836 mL	1.9590 mL
	60 mM	0.0653 mL	0.3265 mL	0.6530 mL	1.6325 mL
	80 mM	0.0490 mL	0.2449 mL	0.4898 mL	1.2244 mL
	100 mM	0.0392 mL	0.1959 mL	0.3918 mL	0.9795 mL

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7,8-Dihydroneopterin

Gut Microbial Metabolite Library

Microbial Metabolite Library

Neurodegenerative Disease-related Compound Library

Angiogenesis-Related Compound Library

Food-Sourced Compound Library

Human Metabolite Library

Anti-Pulmonary Fibrosis Compound Library

Metabolic Enzyme Compound Library

Membrane Protein-targeted Compound Library

Animal-Sourced Natural Product Library

Highly Selective Activators Library

Cell Death Library

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Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

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Mass Spectrometry Human Endogenous Metabolite Library

Mass Spectrometry Human Metabolite Library

Natural Product Diversity Scaffold Library

Bacteria-Sourced Compound Library

Mass Spectrometry Natural Product Library

Natural Product and Natural Product-Like Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

Natural Product Library

Human Endogenous Metabolite Compound Library

Anti-Aging Compound Library

All NO Synthase Isoforms

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

7,8-Dihydroneopterin Related Classifications

HY-136341 Related Classifications