8-Azahypoxanthine
Based on 1 Customer Validation
8-Azahypoxanthine is a purine analog and an inhibitor of hypoxanthine-guanine phosphoribosyltransferase (HGPRT). 8-Azahypoxanthine exhibits antitumor activity and can be used in the research of tumors such as adenocarcinoma.
For research use only. We do not sell to patients.
- Purity: 98.99%
- CAS No.: 2683-90-1
- Formula: C4H3N5O
- Molecular Weight:137.10
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
8-Azahypoxanthine (10 μg/mL) in the medium containing 10% normal FBS reduces the cloning efficiency of HeLa S3 cells to nearly 0 (complete lethality), while exerting no effect on the cloning efficiency of normal diploid fibroblasts[1].
8-Azahypoxanthine can inhibit the colony formation of the human epidermoid carcinoma cell line H. Ep. No. 2 and suppress the growth of Ca 755 cells (cultured mouse adenocarcinoma 755 cells)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BD2F1 mice (C57BL/6×DBA/2 F1 mice) treated Adenocarcinoma 755 (Ca 755) tumors[2]
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Dosage:Non-toxic dose
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Administration:Intraperitoneal injection; Day 1-11
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Result:Inhibited the growth of subcutaneous Ca 755 tumors in BD2F1 mice.
Chemical Information
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CAS No. 2683-90-1
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Appearance Solid
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Molecular Weight 137.10
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Formula C4H3N5O
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Color Light yellow to yellow
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SMILES
O=C1C2=C(N=CN1)NN=N2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (729.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (18.23 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (18.23 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. M H Vaughan, et al. Differential sensitivity of human normal and malignant cells to 8-azahypoxanthine in vitro. Exp Cell Res. 1971, 69, 1. [Content Brief]
[2]. Bennett LL Jr, et al. Studies with 8-azainosine, a cytotoxic nucleoside with antitumor activity. Cancer Res. 1973 Mar;33(3):465-71. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 7.2939 mL | 36.4697 mL | 72.9395 mL | 182.3486 mL |
| 5 mM | 1.4588 mL | 7.2939 mL | 14.5879 mL | 36.4697 mL | |
| 10 mM | 0.7294 mL | 3.6470 mL | 7.2939 mL | 18.2349 mL | |
| 15 mM | 0.4863 mL | 2.4313 mL | 4.8626 mL | 12.1566 mL | |
| 20 mM | 0.3647 mL | 1.8235 mL | 3.6470 mL | 9.1174 mL | |
| 25 mM | 0.2918 mL | 1.4588 mL | 2.9176 mL | 7.2939 mL | |
| 30 mM | 0.2431 mL | 1.2157 mL | 2.4313 mL | 6.0783 mL | |
| 40 mM | 0.1823 mL | 0.9117 mL | 1.8235 mL | 4.5587 mL | |
| 50 mM | 0.1459 mL | 0.7294 mL | 1.4588 mL | 3.6470 mL | |
| 60 mM | 0.1216 mL | 0.6078 mL | 1.2157 mL | 3.0391 mL | |
| 80 mM | 0.0912 mL | 0.4559 mL | 0.9117 mL | 2.2794 mL | |
| 100 mM | 0.0729 mL | 0.3647 mL | 0.7294 mL | 1.8235 mL |
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