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AMZ30 

Cat. No.: HY-12833 Purity: 99.16%
Handling Instructions

AMZ30 is a selective, covalent inhibitors of protein phosphatase methylesterase-1(PME-1; IC50=600 nM); selectively inactivates PME-1 and reduces the demethylated form of PP2A in living cells.

For research use only. We do not sell to patients.

AMZ30 Chemical Structure

AMZ30 Chemical Structure

CAS No. : 1313613-09-0

Size Price Stock Quantity
10 mg USD 144 In-stock
Estimated Time of Arrival: December 31
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Top Publications Citing Use of Products

    AMZ30 purchased from MCE. Usage Cited in: Front Aging Neurosci. 2018 Jun 8;10:173.

    Immunoblotting and quantifications of deMe-PP2A, total-PP2A, p-α-syn, α-syn, and Flag-LCMT-1 in vector, α-syn, α-syn+LCMT-1, α-syn+AMZ30, α-syn+PP2Ac, and α-syn+siPME-1 group. β-tubulin is used as a loading control.
    • Biological Activity

    • Technical Information

    • Purity & Documentation

    • References

    Description

    AMZ30 is a selective, covalent inhibitors of protein phosphatase methylesterase-1(PME-1; IC50=600 nM); selectively inactivates PME-1 and reduces the demethylated form of PP2A in living cells. IC50 value: 600 nM [1] Target: PME-1 inhibitor in vitro: AMZ30 showed substantially improved inhibition of PME-1 (IC50 value of 600 nM) with more than 100-fold selectivity relative to other SHs in human cell lysates. Incubation of HEK 293T cells with a concentration range of AMZ30 generated an in situ IC50 value for PME-1 inhibition of 3.5 μM as determined by gel-based ABPP without any observed cross-reactivity with other SHs even at 100 μM compound. Treatment of HEK 293T cells stably overexpressing PME-1 with 28 (20 μM) caused an ~80% reduction in the levels of demethylated PP2A [1].

    Solvent & Solubility
    In Vitro: 

    DMSO : 4.8 mg/mL (10.40 mM; Need ultrasonic and warming)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1671 mL 10.8356 mL 21.6713 mL
    5 mM 0.4334 mL 2.1671 mL 4.3343 mL
    10 mM 0.2167 mL 1.0836 mL 2.1671 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Molecular Weight

    461.44

    Formula

    C₁₉H₁₂FN₃O₆S₂

    CAS No.

    1313613-09-0

    SMILES

    O=S(C1=CC([N+]([O-])=O)=CC=C1)(N2C(/C=C(S(=O)(C3=CC=C(F)C=C3)=O)\C#N)=CC=C2)=O

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

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    × = ×
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    Product Name:
    AMZ30
    Cat. No.:
    HY-12833
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    AMZ30

    Cat. No.: HY-12833