Corynoxine B
Based on 5 publication(s) in Google Scholar
Corynoxine B is an alkaloid-based autophagy inducer and α-synuclein aggregation inhibitor that ameliorates Mn-induced dysregulation of autophagy and enhances α-synuclein (α-syn) clearance in Parkinson's disease mice.
For research use only. We do not sell to patients.
- Purity: 99.76%
- CAS No.: 17391-18-3
- Formula: C22H28N2O4
- Molecular Weight:384.47
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Corynoxine B
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Customer Validation & Images
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IHC
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WB
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IHC
All α-synuclein Isoforms
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Biological Activity
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α-synuclein Aggregation |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HL-60 | IC50 |
>40 μM
Compound: 5
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Cytotoxicity against human HL60 cells after 48 hrs by MTT assay
Cytotoxicity against human HL60 cells after 48 hrs by MTT assay
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[PMID: 21070010] |
| SW480 | IC50 |
>40 μM
Compound: 5
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Cytotoxicity against human SW480 cells after 48 hrs by MTT assay
Cytotoxicity against human SW480 cells after 48 hrs by MTT assay
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[PMID: 21070010] |
Corynoxine B (25-100 μM, 2 h) can improve Mn induced autophagy dysregulation and neurotoxicity in SH-SY5Y human neuroblastoma cells[1]. Corynoxine B can enhance the expression of BECN1 protein and restore autophagy inhibition caused by overexpression of SNCA/α-synuclein (α-syn) in PC12 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SH-SY5Y cell
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Concentration:25-100 μM
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Incubation Time:2 h
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Result:Improved cell vitality
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Cell Line:Inducible PC12 cell lines (iPC12) overexpressing SNCA (WT and A53T)
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Concentration:
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Incubation Time:24 h
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Result:Increased LC3-II and BECN1 protein levels
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:A53T α-syn transgenic mouse model[3].
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Dosage:5-20 mg/kg
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Administration:Intraperitoneal injection (i.p.)
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Result:Promoted α-syn clearance
Chemical Information
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CAS No. 17391-18-3
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Appearance Solid
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Molecular Weight 384.47
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Formula C22H28N2O4
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Color White to off-white
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SMILES
O=C(NC1=C2C=CC=C1)[C@]32[C@@](C[C@H](/C(C(OC)=O)=C\OC)[C@H](CC)C4)([H])N4CC3
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (5)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
VPS34 Governs Oocyte Developmental Competence by Regulating Mito/Autophagy: A Novel Insight into the Significance of RAB7 Activity and Its Subcellular Location. [Abstract]2024 Sep 17:e2308823. PMID: 39287146 -
Acta Pharmacol Sin
Corynoxine B derivative CB6 prevents Parkinsonian toxicity in mice by inducing PIK3C3 complex-dependent autophagy. [Abstract]2022 Oct;43(10):2511-2526. PMID: 35217810 -
Food Chem Toxicol
Corynoxine B ameliorates HMGB1-dependent autophagy dysfunction during manganese exposure in SH-SY5Y human neuroblastoma cells. [Abstract]2019 Feb;124:336-348. PMID: 30578841
Corynoxine B purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2019 Feb;124:336-348. [Abstract]
The expression levels of Beclin1, LC3II/I, and p62 are determined by western blotting in the control, 200 μM Mn treatment, and Cory B pretreatment groups.
Corynoxine B purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2019 Feb;124:336-348. [Abstract]
Expression levels of α-SYN and Bcl2 in the whole-cell lysates are determined by western blotting in the control, 200 μM Mn treatment, and Cory B pretreatment groups.
Corynoxine B purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2019 Feb;124:336-348. [Abstract]
The colocalization of HMGB1 (red) and Beclin1 (green), and Bcl2 (red) and Beclin1 (green) in the control, 200 μM Mn treatment, and 100 μM Cory B pretreatment groups.
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J Pharm Pharmacol
Advanced oxidation protein products accelerates paracetamol-induced liver injury through AMPK-mTOR signaling pathway in chronic kidney disease. [Abstract]2025 Aug 19:rgaf069. PMID: 40828098 -
Plant Biotechnol (Tokyo)
A sterile plant culture system of Uncaria rhynchophylla as a biosynthetic model of monoterpenoid indole alkaloids. [Abstract]2025 Jun 25;42(2):145-154. PMID: 40636431
Solvent & Solubility
DMSO : 83.33 mg/mL (216.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.41 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Portuguese - PT (479 KB)
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Handling Instructions (2659 KB)
References
[1]. Yan D, et al. Corynoxine B ameliorates HMGB1-dependent autophagy dysfunction during manganese exposure in SH-SY5Y human neuroblastoma cells. Food Chem Toxicol. 2019 Feb;124:336-348. [Content Brief]
[2]. Chen L, et al. Corynoxine Protects Dopaminergic Neurons Through Inducing Autophagy and Diminishing Neuroinflammation in Rotenone-Induced Animal Models of Parkinson's Disease. Front Pharmacol. 2021 Apr 13;12:642900. [Content Brief]
[3]. Song JX, et al. HMGB1 is involved in autophagy inhibition caused by SNCA/α-synuclein overexpression: a process modulated by the natural autophagy inducer corynoxine B. Autophagy. 2014 Jan;10(1):144-54. doi: 10.4161/auto.26751. Epub 2013 Jan 1. Erratum in: Autophagy. 2015;11(9):1708. [Content Brief]
[4]. Zhu Q, et al. Corynoxine B targets at HMGB1/2 to enhance autophagy for α-synuclein clearance in fly and rodent models of Parkinson's disease. Acta Pharm Sin B. 2023 Jun;13(6):2701-2714. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6010 mL | 13.0049 mL | 26.0098 mL | 65.0246 mL |
| 5 mM | 0.5202 mL | 2.6010 mL | 5.2020 mL | 13.0049 mL | |
| 10 mM | 0.2601 mL | 1.3005 mL | 2.6010 mL | 6.5025 mL | |
| 15 mM | 0.1734 mL | 0.8670 mL | 1.7340 mL | 4.3350 mL | |
| 20 mM | 0.1300 mL | 0.6502 mL | 1.3005 mL | 3.2512 mL | |
| 25 mM | 0.1040 mL | 0.5202 mL | 1.0404 mL | 2.6010 mL | |
| 30 mM | 0.0867 mL | 0.4335 mL | 0.8670 mL | 2.1675 mL | |
| 40 mM | 0.0650 mL | 0.3251 mL | 0.6502 mL | 1.6256 mL | |
| 50 mM | 0.0520 mL | 0.2601 mL | 0.5202 mL | 1.3005 mL | |
| 60 mM | 0.0433 mL | 0.2167 mL | 0.4335 mL | 1.0837 mL | |
| 80 mM | 0.0325 mL | 0.1626 mL | 0.3251 mL | 0.8128 mL | |
| 100 mM | 0.0260 mL | 0.1300 mL | 0.2601 mL | 0.6502 mL |
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