Cat. No.: HY-N0006

Demethoxycurcumin

(Synonyms: Curcumin II; Desmethoxycurcumin; Monodemethoxycurcumin)

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

Demethoxycurcumin is one of the main active ingredients of curcumin, which has anti-inflammatory, antioxidant, antibacterial, anti-cancer and neuroprotective effects. Demethoxycurcumin is orally active. Demethoxycurcumin can be used in inflammation, cancer and Alzheimer's disease research.

For research use only. We do not sell to patients.

Purity: 99.95%
CAS No.: 22608-11-3
Formula: C₂₀H₁₈O₅
Molecular Weight:338.36

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Demethoxycurcumin Chemical Structure

CAS No.: 22608-11-3

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

ChAT

iNOS

All Cholinesterase (ChE) Isoforms :

AChE
BChE

All NO Synthase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A-431	IC₅₀	>100 μM Compound: DC	Cytotoxicity against human A431 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay Cytotoxicity against human A431 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay	[PMID: 29605808]
A549	IC₅₀	28.2 μM Compound: DC	Cytotoxicity against human A549 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay Cytotoxicity against human A549 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay	[PMID: 29605808]
DLD-1	IC₅₀	>40 μM Compound: DC	Cytotoxicity against human DLD1 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay Cytotoxicity against human DLD1 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay	[PMID: 29605808]
HT-1080	IC₅₀	7.5 μM Compound: DMC	Decrease in UPA expression in human HT1080 cells Decrease in UPA expression in human HT1080 cells	[PMID: 31336310]
MCF7	IC₅₀	92.1 μM Compound: DC	Cytotoxicity against human MCF7 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay Cytotoxicity against human MCF7 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay	[PMID: 29605808]
MDA-MB-231	IC₅₀	9 mM Compound: DMC	Increase in MMP3 level in human MDA-MB-231 cells after 24 hrs by ELISA Increase in MMP3 level in human MDA-MB-231 cells after 24 hrs by ELISA	[PMID: 31336310]
WRL68	IC₅₀	26.3 μM Compound: DC	Cytotoxicity against human WRL68 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay Cytotoxicity against human WRL68 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay	[PMID: 29605808]

In Vitro

Demethoxycurcumin (0-150 μM, 0-96 h) exerts anti-inflammatory effect on Caco-2 cells of in vitro inflamed human intestinal mucosa model by inhibiting NO secretion induced by iNOS^[1].
Demethoxycurcumin (35 μM, 48 h) can change the expression of DNA damage, cell cycle and apoptosis-related genes in human lung cancer NCI-H460 cells, and exhibit anticancer activity^[2].
The IC₅₀ values of Demethoxycurcumin (2-1024 μg/mL, 12-14 h) forE. coli, S. aureus, and S. dysenteriae are 212.5, 282.5 and 465.0 μg/mL, and the MIC values are 512, 1024 and 1024 μg/mL, respectively^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Caco-2 Cells (LPS-induced inflammation model cell)
Concentration:

0-150 μM
Incubation Time:

0-96 h
Result:

Showed cytotoxic effects on CACO-2-differentiated cells at 130 μM and 150 μM concentrations.

In Vivo

Demethoxycurcumin (3-30 mg/kg/day for 5 days or 20 days, i.p.) regulates the expression of inflammatory and apoptotic genes in the hippocampus and frontal cortex of rat models infused with Aβ + Ibotenic acid (HY-N2311) and helps to improve Alzheimer's disease^[3].
Demethoxycurcumin (10 mg/kg/day for 28 days, p.o.) can enhance cognition and enhance choline acetyltransferase (ChAT) activity in mice induced by Scopolamine (HY-N0296) ^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male ICR mice (6 weeks old) weighing 30–35 g, memory impairment induced by Scopolamine^[5]
Dosage:

10 mg/kg/day for 28 days
Administration:

Oral gavage (p.o.)
Result:

Significantly improved scopolamine induced passive avoidance task learning disabilities and memory impairments in Morris Water Maze.
The activity of choline acetyltransferase (ChAT) was significantly increased to 33.03%.

Chemical Information

CAS No. 22608-11-3
Appearance Solid
Molecular Weight 338.36
Formula C₂₀H₁₈O₅
Color Pink to red
SMILES

O=C(CC(/C=C/C1=CC=C(O)C=C1)=O)/C=C/C2=CC=C(O)C(OC)=C2
Synonyms

Curcumin II; Desmethoxycurcumin; Monodemethoxycurcumin
Structure Classification
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (5)

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Journal Impact Factor

Imeta

2026 Feb 24.
Cancer Gene Ther

Demethoxycurcumin suppresses HK2-mediated glycolysis by targeting PTEN/Akt signaling. [Abstract]

2025 Oct 2. PMID: 41039015
Philos Trans R Soc Lond B Biol Sci

Small molecule activates citrullination through targeting PAD2. [Abstract]

2023 Nov 20;378(1890):20220248. PMID: 37778388
Future Pharmacol

2024 Mar 8, 4(1), 256-278.
Vet Microbiol

Curcumin inhibits classical swine fever virus replication by interfering with lipid metabolism. [Abstract]

2021 Aug;259:109152. PMID: 34146894

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (295.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9554 mL

14.7772 mL

29.5543 mL

5 mM

0.5911 mL

2.9554 mL

5.9109 mL

10 mM

0.2955 mL

1.4777 mL

2.9554 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 3.25 mg/mL (9.61 mM); Clear solution

This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (459 KB)
- English - EN (459 KB)
- Français - FR (459 KB)
- Deutsch - DE (459 KB)
- Norwegian - NO (459 KB)
- Español - ES (459 KB)
- Swedish - SV (459 KB)
- Italian - IT (459 KB)
- Korean - KR (459 KB)
- Portuguese - PT (459 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Somchit M, et al. Demethoxycurcumin from Curcuma longa rhizome suppresses iNOS induction in an in vitro inflamed human intestinal mucosa model. Asian Pac J Cancer Prev. 2014;15(4):1807-10. [Content Brief]

[2]. Ko YC, et al. Demethoxycurcumin Alters Gene Expression Associated with DNA Damage, Cell Cycle and Apoptosis in Human Lung Cancer NCI-H460 Cells In Vitro. In Vivo. 2015 01-02;29(1):83-94. [Content Brief]

[3]. Ahmed T, et al. A comparative study of curcuminoids to measure their effect on inflammatory and apoptotic gene expression in an Aβ plus ibotenic acid-infused rat model of Alzheimer's disease. Brain Res. 2011 Jul 11;1400:1-18. [Content Brief]

[4]. Lim DW, et al. Enhanced Cognitive Effects of Demethoxycurcumin, a Natural Derivative of Curcumin on Scopolamine-Induced Memory Impairment in Mice. Molecules. 2016 Aug 5;21(8):1022. [Content Brief]

[5]. Luo J, et al. Demethoxycurcumin: A potential antimicrobial agent: Exposure by microcalorimetry and modified broth microdilution method[J]. Journal of Thermal Analysis and Calorimetry, 2014, 115: 2331-2338.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9554 mL	14.7772 mL	29.5543 mL	73.8858 mL
	5 mM	0.5911 mL	2.9554 mL	5.9109 mL	14.7772 mL
	10 mM	0.2955 mL	1.4777 mL	2.9554 mL	7.3886 mL
	15 mM	0.1970 mL	0.9851 mL	1.9703 mL	4.9257 mL
	20 mM	0.1478 mL	0.7389 mL	1.4777 mL	3.6943 mL
	25 mM	0.1182 mL	0.5911 mL	1.1822 mL	2.9554 mL
	30 mM	0.0985 mL	0.4926 mL	0.9851 mL	2.4629 mL
	40 mM	0.0739 mL	0.3694 mL	0.7389 mL	1.8471 mL
	50 mM	0.0591 mL	0.2955 mL	0.5911 mL	1.4777 mL
	60 mM	0.0493 mL	0.2463 mL	0.4926 mL	1.2314 mL
	80 mM	0.0369 mL	0.1847 mL	0.3694 mL	0.9236 mL
	100 mM	0.0296 mL	0.1478 mL	0.2955 mL	0.7389 mL

Keywords

Demethoxycurcumin

5 Cited Publications

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Demethoxycurcumin

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Publications Citing Use of MedChemExpress (MCE) Demethoxycurcumin

All Cholinesterase (ChE) Isoforms

All NO Synthase Isoforms

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Demethoxycurcumin Related Classifications

Cat. No.: HY-N0006