Methylcobalamin
Based on 6 publication(s) in Google Scholar
Methylcobalamin (CH3-B12), a cobalamin, is a form of vitamin B12.
For research use only. We do not sell to patients.
- Purity: 99.02%
- CAS No.: 13422-55-4
- Formula: C63H91CoN13O14P
- Molecular Weight:1344.38
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Methylcobalamin
More- Nat Commun. 2025 Jan 31;16(1):1233. [Abstract]
- Adv Sci (Weinh). 2025 Oct 27:e13775. [Abstract]
- Phytomedicine. 2026 Apr:153:157899. [Abstract]
- Virol Sin. 2026 May 29:S1995-820X(26)00084-2. [Abstract]
- J Integr Neurosci. 2025 Jun 24;24(6):36965. [Abstract]
- J Cell Biochem. 2019 Jul;120(7):11921-11930. [Abstract]
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All Endogenous Metabolite Isoforms
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Biological Activity
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Human Endogenous Metabolite |
Methylcobalamin (CH3-B12) is a cobalamin, a form of vitamin B12. It differs from cyanocobalamin in that the cyanide is replaced by a methyl group. Methylcobalamin (CH3-B12) features an octahedral cobalt(III) centre. Methylcobalamin (CH3-B12) can be obtained as bright red crystals. From the perspective of coordination chemistry, Methylcobalamin (CH3-B12) is notable as a rare example of a compound that contains metal-alkyl bonds. Methylcobalamin (CH3-B12) is equivalent physiologically to vitamin B12, and can be used to prevent or treat pathology arising from a lack of vitamin B12 (vitamin B12 deficiency), such as pernicious anemia. Methylcobalamin (CH3-B12) is also used in the treatment of peripheral neuropathy, diabetic neuropathy, and as a preliminary treatment for amyotrophic lateral sclerosis. From Wikipedia.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 13422-55-4
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Appearance Solid
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Molecular Weight 1344.38
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Formula C63H91CoN13O14P
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Color Brown to red
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SMILES
C[C@]1([C@](C)2CC(N)=O)[N]([Co+3]([N](C([C@](C)3CC(N)=O)=C4C)=C5[C@H]3CCC(N)=O)([N](C(C(C)6C)=C5)=C([C@H]6CCC(N)=O)C(C)=C7[C@@]8(CCC(NC[C@H]9C)=O)C)([N](C%10=C%11)=CN([C@@H](O[C@@H]%12CO)[C@]([C@@H]%12O[P]([O-])(O9)=O)([H])O)C%10=CC(C)=C%11C)[N-]7[C@]1([H])[C@@H]8CC(N)=O)=C4[C@H]2CCC(N)=O.[CH3-]
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Synonyms
Mecobalamin; CH3-B12
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (6)
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Journal Impact Factor
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Most Recent
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Nat Commun
2025 Jan 31;16(1):1233. PMID: 39890804
Methylcobalamin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233. [Abstract]
MST measurements of the binding of 0.2 μM recombinant caspase-3, GSDME, GSDMD with Methylcobalamin (MeCbl) or positive control. Methylcobalamin (MeCbl) specifically bound to GSDME but not to caspase-3 or GSDMD.
Methylcobalamin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233. [Abstract]
Representative immunoblot of GSDME pretreated with inhibitors as indicated, and then incubated with recombinant active caspase-3 for GSDME activation analysis. GSDME cleavage by caspase-3 was potently disrupted by Methylcobalamin (MeCbl) (1 μM; 30 min).
Methylcobalamin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233. [Abstract]
Changes in HepG2 cell morphology were observed with a microscope. Methylcobalamin (MeCbl) (20 μM; 2h) obviously blocked the appearance of pyroptotic bubbles (red arrow).
Methylcobalamin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233. [Abstract]
SYTOX fluorescence probe was used to investigate the protective effects of Methylcobalamin (MeCbl) (20 μM) on DCA-induced systemic and liver injury. The liver region was indicated with a red or yellow circle. Methylcobalamin (MeCbl) was effective against DCA-induced liver damage of zebrafish.
Methylcobalamin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233. [Abstract]
Methylcobalamin (MeCbl) (10 mg/kg; i.g.; once daily) treatment drastically reduced Serum transaminase ALT/AST activities, IL-1α and IL-1β in wild-type six-week-old C57BL/6J male mice.
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Adv Sci (Weinh)
VDLIN: A Deep Learning-Based Platform for Methylcobalamin-Inspired Immunomodulatory Compound Screening. [Abstract]2025 Oct 27:e13775. PMID: 41144841 -
Phytomedicine
Baohuoside I induces GSDME-dependent pyroptosis and synergistically inhibits lung adenocarcinoma with cisplatin. [Abstract]2026 Apr:153:157899. PMID: 41643445 -
Virol Sin
EV-A71 induces GSDME-dependent T-cell pyroptosis: A novel mechanism and MeCbl therapeutic potential. [Abstract]2026 May 29:S1995-820X(26)00084-2. PMID: 42217583 -
J Integr Neurosci
Conical Chitosan Conduits Combined With Methylcobalamin for Sciatic Nerve Transposition Repair. [Abstract]2025 Jun 24;24(6):36965. PMID: 40613369 -
J Cell Biochem
2019 Jul;120(7):11921-11930. PMID: 30793354
Solvent & Solubility
DMSO : 50 mg/mL (37.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (3.72 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (1.86 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (1.86 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 18.75 mg/mL (13.95 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (287 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 0.7438 mL | 3.7192 mL | 7.4384 mL | 18.5959 mL |
| DMSO | 5 mM | 0.1488 mL | 0.7438 mL | 1.4877 mL | 3.7192 mL |
| 10 mM | 0.0744 mL | 0.3719 mL | 0.7438 mL | 1.8596 mL | |
| 15 mM | 0.0496 mL | 0.2479 mL | 0.4959 mL | 1.2397 mL | |
| 20 mM | 0.0372 mL | 0.1860 mL | 0.3719 mL | 0.9298 mL | |
| 25 mM | 0.0298 mL | 0.1488 mL | 0.2975 mL | 0.7438 mL | |
| 30 mM | 0.0248 mL | 0.1240 mL | 0.2479 mL | 0.6199 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Methylcobalamin Related Classifications
HY-B0586 Related Classifications
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