Methylcobalamin (Synonyms: Mecobalamin; CH3-B12)

Name: Methylcobalamin
Brand: MedChemExpress
SKU: HY-B0586
Rating: 5.0 (5 reviews)

Cat. No.: HY-B0586 Purity: 99.02%: Data Sheet
COA

SDS
Handling Instructions
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Methylcobalamin (CH3-B12), a cobalamin, is a form of vitamin B12.

For research use only. We do not sell to patients.

CAS No. : 13422-55-4

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Methylcobalamin:

5 Publications Citing Use of MCE Methylcobalamin

Bio/Physico-chemical Assay

Cell Imaging/Staining

In Vivo Efficacy Study

: Methylcobalamin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233. [Abstract]; MST measurements of the binding of 0.2 μM recombinant caspase-3, GSDME, GSDMD with Methylcobalamin (MeCbl) or positive control. Methylcobalamin (MeCbl) specifically bound to GSDME but not to caspase-3 or GSDMD.

: Methylcobalamin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233. [Abstract]; Representative immunoblot of GSDME pretreated with inhibitors as indicated, and then incubated with recombinant active caspase-3 for GSDME activation analysis. GSDME cleavage by caspase-3 was potently disrupted by Methylcobalamin (MeCbl) (1 μM; 30 min).

: Methylcobalamin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233. [Abstract]; Changes in HepG2 cell morphology were observed with a microscope. Methylcobalamin (MeCbl) (20 μM; 2h) obviously blocked the appearance of pyroptotic bubbles (red arrow).

: Methylcobalamin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233. [Abstract]; SYTOX fluorescence probe was used to investigate the protective effects of Methylcobalamin (MeCbl) (20 μM) on DCA-induced systemic and liver injury. The liver region was indicated with a red or yellow circle. Methylcobalamin (MeCbl) was effective against DCA-induced liver damage of zebrafish.

: Methylcobalamin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233. [Abstract]; Methylcobalamin (MeCbl) (10 mg/kg; i.g.; once daily) treatment drastically reduced Serum transaminase ALT/AST activities, IL-1α and IL-1β in wild-type six-week-old C57BL/6J male mice.

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Description

Methylcobalamin (CH3-B12), a cobalamin, is a form of vitamin B12.

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Methylcobalamin (CH3-B12) is a cobalamin, a form of vitamin B12. It differs from cyanocobalamin in that the cyanide is replaced by a methyl group. Methylcobalamin (CH3-B12) features an octahedral cobalt(III) centre. Methylcobalamin (CH3-B12) can be obtained as bright red crystals. From the perspective of coordination chemistry, Methylcobalamin (CH3-B12) is notable as a rare example of a compound that contains metal-alkyl bonds. Methylcobalamin (CH3-B12) is equivalent physiologically to vitamin B12, and can be used to prevent or treat pathology arising from a lack of vitamin B12 (vitamin B12 deficiency), such as pernicious anemia. Methylcobalamin (CH3-B12) is also used in the treatment of peripheral neuropathy, diabetic neuropathy, and as a preliminary treatment for amyotrophic lateral sclerosis. From Wikipedia.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

1344.38

Formula

C₆₃H₉₁CoN₁₃O₁₄P

CAS No.

13422-55-4

Appearance

Solid

Color

Brown to red

SMILES

C[C@]1([C@](C)2CC(N)=O)[N]([Co+3]([N](C([C@](C)3CC(N)=O)=C4C)=C5[C@H]3CCC(N)=O)([N](C(C(C)6C)=C5)=C([C@H]6CCC(N)=O)C(C)=C7[C@@]8(CCC(NC[C@H]9C)=O)C)([N](C%10=C%11)=CN([C@@H](O[C@@H]%12CO)[C@]([C@@H]%12O[P]([O-])(O9)=O)([H])O)C%10=CC(C)=C%11C)[N-]7[C@]1([H])[C@@H]8CC(N)=O)=C4[C@H]2CCC(N)=O.[CH3-]

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (37.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 5 mg/mL (3.72 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.7438 mL	3.7192 mL	7.4384 mL
5 mM	0.1488 mL	0.7438 mL	1.4877 mL
10 mM	0.0744 mL	0.3719 mL	0.7438 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (1.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (1.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (1.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 18.75 mg/mL (13.95 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 100.00%

Select Batch:

Data Sheet (287 KB) SDS (393 KB)

COA (276 KB)

Handling Instructions (2659 KB)

References

[1]. http://en.wikipedia.org/wiki/Methylcobalamin

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	0.7438 mL	3.7192 mL	7.4384 mL	18.5959 mL
DMSO	5 mM	0.1488 mL	0.7438 mL	1.4877 mL	3.7192 mL
	10 mM	0.0744 mL	0.3719 mL	0.7438 mL	1.8596 mL
	15 mM	0.0496 mL	0.2479 mL	0.4959 mL	1.2397 mL
	20 mM	0.0372 mL	0.1860 mL	0.3719 mL	0.9298 mL
	25 mM	0.0298 mL	0.1488 mL	0.2975 mL	0.7438 mL
	30 mM	0.0248 mL	0.1240 mL	0.2479 mL	0.6199 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Methylcobalamin Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methylcobalamin13422-55-4Mecobalamin CH3-B12Endogenous MetaboliteInhibitorinhibitorinhibit

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Methylcobalamin (Synonyms: Mecobalamin; CH3-B12)

Customer Review

Other Forms of Methylcobalamin:

Methylcobalamin Related Antibodies

5 Publications Citing Use of MCE Methylcobalamin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Methylcobalamin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Methylcobalamin Related Classifications

Keywords:

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Customer Review

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