Cat. No.: HY-N1499

Nystose

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

Nystose is a tetrasaccharide with two fructose molecules linked via beta (1→2) bonds to the fructosyl moiety of sucrose. Nystose exhibits prebiotic, immunomodulatory, and metabolism regulating activities. Nystose promotes the bone mineralization by activating the Wnt/β-catenin signaling pathway.

For research use only. We do not sell to patients.

Purity: 99.98%
CAS No.: 13133-07-8
Formula: C₂₄H₄₂O₂₁
Molecular Weight:666.58

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Nystose Chemical Structure

CAS No.: 13133-07-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Wnt Isoforms :

All TGF-beta/Smad Isoforms :

Smad1
Smad3

In Vitro

Nystose can fermented by lactobacilli (L. intestinalis and L. reuteri), and promotes the proliferation of lactobacilli^[2].
Nystose (0.1-10 nM, 48 h) increases the activity of ALP and the proliferation of BMSCs, promotes the bone mineralization by activating the Wnt/β-catenin signaling pathway^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:

BMSCs
Concentration:

0.1 nM, 1 nM, 10 nM
Incubation Time:

48 h
Result:

Increased expression of osterix and p-Smad1/5/8.

In Vivo

Nystose (100 mg/kg, po for 14 days) enhances the intestinal IgA secretion and modulates systemic immune responses in BALB/c mouse models^[2].
Nystose (40-160 mg/kg, po for 8 weeks) improves the bone density and bone microstructure in mouse models^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c mouse models^[2]
Dosage:

100 mg/kg
Administration:

po for 14 days
Result:

Inhibited the secretion of IL-2, IFN-γ, IL-12 and IL-4 by splenocytes.

Animal Model:

ICR mouse models^[3]
Dosage:

40-160 mg/kg
Administration:

po for 8 weeks
Result:

Improved the bone density and bone microstructure.

Chemical Information

CAS No. 13133-07-8
Appearance Solid
Molecular Weight 666.58
Formula C₂₄H₄₂O₂₁
Color White to off-white
SMILES

O[C@@H]1[C@@](CO[C@@]2(O[C@H](CO)[C@@H](O)[C@@H]2O)CO[C@@]3(O[C@H](CO)[C@@H](O)[C@@H]3O)CO)(O[C@H](CO)[C@H]1O)O[C@H]([C@@H]([C@@H](O)[C@@H]4O)O)O[C@@H]4CO
Structure Classification
- Saccharides
- Polysaccharides
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (1)

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Journal Impact Factor

University of Maryland

2026.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (150.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 83.33 mg/mL (125.01 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.5002 mL

7.5010 mL

15.0019 mL

5 mM

0.3000 mL

1.5002 mL

3.0004 mL

10 mM

0.1500 mL

0.7501 mL

1.5002 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (3.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (150.02 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ziesenitz SC, et al. In vitro assessment of nystose as a sugar substitute. J Nutr. 1987 May;117(5):846-51. [Content Brief]

[2]. N Yoshida, et al., Effects of 1-kestose and nystose on the intestinal microorganisms and immune system in mice[J]. Journal of applied Glycoscience, 2006, 53(3): 175-180.

[3]. Zhang Q, et al. Nystose attenuates bone loss and promotes BMSCs differentiation to osteoblasts through BMP and Wnt/β-catenin pathway in ovariectomized mice[J]. Food Science and Human Wellness, 2023, 12(2): 634-646.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	1.5002 mL	7.5010 mL	15.0019 mL	37.5049 mL
	5 mM	0.3000 mL	1.5002 mL	3.0004 mL	7.5010 mL
	10 mM	0.1500 mL	0.7501 mL	1.5002 mL	3.7505 mL
	15 mM	0.1000 mL	0.5001 mL	1.0001 mL	2.5003 mL
	20 mM	0.0750 mL	0.3750 mL	0.7501 mL	1.8752 mL
	25 mM	0.0600 mL	0.3000 mL	0.6001 mL	1.5002 mL
	30 mM	0.0500 mL	0.2500 mL	0.5001 mL	1.2502 mL
	40 mM	0.0375 mL	0.1875 mL	0.3750 mL	0.9376 mL
	50 mM	0.0300 mL	0.1500 mL	0.3000 mL	0.7501 mL
	60 mM	0.0250 mL	0.1250 mL	0.2500 mL	0.6251 mL
	80 mM	0.0188 mL	0.0938 mL	0.1875 mL	0.4688 mL
	100 mM	0.0150 mL	0.0750 mL	0.1500 mL	0.3750 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Nystose

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Publications Citing Use of MedChemExpress (MCE) Nystose

All Wnt Isoforms

All TGF-beta/Smad Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Nystose Related Classifications

HY-N1499 Related Classifications