2. Signaling Pathways
  3. Others
  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-NP0188
    Soybean Agglutinin (Biotinylated)
    Soybean Agglutinin (SBA) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Soybean Agglutinin (SBA) Biotinylated is a biological material or organic compound that can be used in life science research.
    Soybean Agglutinin (Biotinylated)
  • HY-D1848A
    Sulfo-Cy3-NHS ditriethylamine
    Sulfo-Cy3-NHS ditriethylamine is a derivative of Cy3 (Cyanine3) (HY-D0822) dye containing sulfonate ions. Sulfo-Cy3-NHS ditriethylamine contains sulfonate ions and has improved water solubility. Sulfo-Cy3-NHS ditriethylamine can label biomolecules containing amine groups, such as proteins, antibodies or peptides.
    Sulfo-Cy3-NHS ditriethylamine
  • HY-D0818
    CY3-YNE
    		98.07%
    CY3-YNE (Sulfo-Cyanine3-alkyne) is a dye for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. CY3-YNE is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
    CY3-YNE
  • HY-DY1095
    Fluo-3AM (solution)
    Fluo-3AM (solution) is a fluorecent Ca2+ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence.
    Solvent and concentration: DMSO: 5 mM
    Fluo-3AM (solution)
  • HY-160840
    SPB-AAD
    SPB-AAD is a BFPX probe. SPB-AAD has covalent capture of in vitro assembled MEF2/DNA and NFAT1/DNA complexes.
    SPB-AAD
  • HY-D1223
    AF488 NHS ester diTEA
    AF488 NHS ester diTEA is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells. AF488 exhibits the λem and λex wavelength of 520 nm and 470 nm, respectively.
    AF488 NHS ester diTEA
  • HY-160869
    Firefly luciferase-IN-2
    Firefly luciferase-IN-2 (compound 5j) is an inhibitor of ATP-dependent luciferase (Firefly luciferase), the major light-emitting enzyme in fireflies and kowtow bugs. Firefly luciferase-IN-2 inhibits P. pyralis luciferase with an IC50 of 0.15 μM, while the inhibitory potency against R. reniformis is not significant.
    Firefly luciferase-IN-2
  • HY-15562
    HOE 32021
    		99.48%
    HOE 32021 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
    HOE 32021
  • HY-D2878
    MitoPeDPP
    MitoPeDPP is a mitochondrial-targeted fluorescent probe that is sensitive to LPO. MitoPeDPP is synthesized from diphenylpyrenephosphine. MitoPeDPP can be used to study the occurrence of mitochondrial LPO in RSL3-induced oligodendrocyte ferroptosis.
    MitoPeDPP
  • HY-D1214
    Basic Red 46
    Basic Red 46 is an alkaline dye that can be removed from aqueous solutions in intermittent adsorption systems using tree ferns as biosorbents.
    Basic Red 46
  • HY-D0363
    Pigment Red 166
    Pigment Red 166 is a commonly used commercial dye for bulk coloring of plastics and synthetic fibers and for surface coatings such as paints and inks.
    Pigment Red 166
  • HY-D2433
    Glucose-PEG2000-Cy3
    Glucose-PEG2000-Cy3 is a Cy3 (HY-D0822) labeled Glucose-PEG conjugate. The Cy3 fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Cy3 has an emission maximum around 562-570 nm. Glucose-PEG improves drug cellular uptake and reduces endosomal degradation, and can be used in drug delivery.
    Glucose-PEG2000-Cy3
  • HY-D2439
    RGD-PEG-Cy3
    		98.50%
    RGD-PEG-Cy3 is a fluorescent labeling reagent that combines Cy3 fluorescent dye, peptide (RGD) and polyethylene glycol (PEG). The Cy3 fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Cy3 has an emission maximum around 562-570 nm. RGD is a peptide sequence (CRGDKGPDCiRGD) that binds to αvβ3 and αvβ5 integrin receptors on tumor neovasculogenesis to achieve specific tumor tissue targeting.
    RGD-PEG-Cy3
  • HY-W440914C
    DSPE-PEG600-FITC
    DSPE-PEG600-FITC is an amphiphile PEG polymer which has unsaturated lipid tails. DSPE-PEG600-FITC can form micelles in an aqueous solution and can be used to prepare liposome/nano particles for drug nanocarriers (Ex/Em=488/525 nm).
    DSPE-PEG600-FITC
  • HY-W026772S1
    Fluorene-d8
    Fluorene-d8 is the deuterium labeled Fluorene (HY-W026772). Fluorene is an orally active polycyclic aromatic hydrocarbon (PAH) and a precursor to other fluorene-based compounds. Fluorene and its derivatives serve as dye precursors for fluorene synthesis. In A549 cells, Fluorene induces oxidative stress and inflammatory responses by increasing ROS and SOD generation, exacerbating lipid peroxidation, modulating antioxidant enzyme activity, and upregulating the expression of pro-inflammatory factors TNF-α and IL-6. In vivo, Fluorene exhibits anxiolytic activity. Fluorene holds potential for research in inflammation and neurological disorders.
    Fluorene-d<sub>8</sub>
  • HY-169491
    Pantothenate-AMC
    		99.27%
    Pantothenate-AMC is a fluorogenic substrate used for detecting the enzymatic activity of vanin-1, an enzyme involved in the recycling of L-pantothenic acid. Upon enzymatic cleavage by vanin-1, 7-amino-7-methylcoumarin (AMC) is released, and its fluorescence can be used to quantify vanin-1 activity. The excitation/emission maxima of AMC are 340-360 nm and 440-460 nm, respectively.
    Pantothenate-AMC
  • HY-R00293F
    FAM-hsa-miR-147b-5p mimic
    FAM-hsa-miR-147b-5p mimic is a FAM-labeled hsa-miR-147b-5p mimic (HY-R00293). hsa-miR-147b-5p mimics are small, chemically synthesized double-stranded RNAs that mimic endogenous miRNAs and enable miRNA functional analysis by up-regulation of miRNA activity.
    FAM-hsa-miR-147b-5p mimic
  • HY-D2645
    Cy5 HA (MW 100000)
    Cy5 HA (MW 100000) is a hyaluronic acid labeled with CY5 (HY-D0821). Cy5 HA (MW 100000) is widely used for cell imaging and in vivo imaging of shallow tissue (Ex/Em = 633/670 nm).
    Cy5 HA (MW 100000)
  • HY-W140203
    2-(2′-Hydroxyphenyl)benzimidazole
    		99.92%
    2-(2′-Hydroxyphenyl) benzimidazole is the most extensively studied excited-state intramolecular proton transfer (ESIPT) molecule, exhibiting normal and tautomer emissions. 2-(2′-Hydroxyphenyl) benzimidazole has been applied as a fluorescent probe in various systems.
    2-(2′-Hydroxyphenyl)benzimidazole
  • HY-DN0194F
    Cy3-Asiatic acid
    Cy3-Asiatic acid is a fluorescent labeling reagent that combines Cy3 (HY-D0822) and Asiatic acid (HY-N0194). Cy3-Asiatic acid can be used to observe and track Asiatic acid under fluorescence microscopy (Ex/Em = 550/570 nm).
    Cy3-Asiatic acid
Cat. No. Product Name / Synonyms Application Reactivity