(±)-Stylopine
Based on 2 publication(s) in Google Scholar
(±)-Stylopine (Tetrahydrocoptisine) is an alkaloid compound. (±)-Stylopine can be isolated from the tubers of the plant Corydalis. (±)-Stylopine inhibits TNF-α, IL-6, and NO production, and attenuates phosphorylation of p38 MAPK, ERK1/2. (±)-Stylopine inhibits NF-κB expression. (±)-Stylopine exhibits anti-inflammatory activity. (±)-Stylopine has protective effects against foot edema, gastric ulcers, anxiety, depression, and acute lung injury.
For research use only. We do not sell to patients.
- Purity: 99.86%
- CAS No.: 4312-32-7
- Formula: C19H17NO4
- Molecular Weight:323.34
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) (±)-Stylopine
More
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| GES1 | IC50 |
>150 μM
Compound: 1
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Antiproliferative activity against human GES1 cells assessed as reduction in cell viability after 24 hrs by MTT assay
Antiproliferative activity against human GES1 cells assessed as reduction in cell viability after 24 hrs by MTT assay
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[PMID: 35286954] |
| HGC-27 | IC50 |
55.06 μM
Compound: 1
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Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability after 24 hrs by MTT assay
Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability after 24 hrs by MTT assay
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[PMID: 35286954] |
| MGC-803 | IC50 |
107.3 μM
Compound: 1
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Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 hrs by MTT assay
Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 hrs by MTT assay
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[PMID: 35286954] |
(±)-Stylopine (10-4 -1 μg/mL; 24 h pretreatment before 12 h LPS stimulation) significantly inhibits LPS-induced TNF-α, IL-6 production[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Mouse peritoneal macrophages
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Concentration:10-4 μg/mL, 10-2 μg/mL, 1 μg/mL
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Incubation Time:24 h
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Result:Reduced phosphorylation of p38MAPK and ERK1/2.
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Cell Line:Mouse peritoneal macrophages
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Concentration:0-4 μg/mL, 10-3 μg/mL, 10-2 μg/mL, 10-1 μg/mL, 1 μg/mL
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Incubation Time:24 h
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Result:Downregulated TNF-α and IL-6 mRNA.
(±)-Stylopine (10-20 mg/kg; i.p.; 3 days) attenuates ethanol-induced gastric ulcer in mice, reduces NO, TNF-α, IL-6 levels, MPO activity and NF-κB expression in gastric tissue and serum[2].
(±)-Stylopine (18.4-36.8 mg/kg; i.p.; 14 days) alleviates LPS-induced neuroinflammation in mice, improves anxiety, depression and anhedonia, reduces neurodegeneration, inhibits KMO expression, NO and lipid peroxidation[3].
(±)-Stylopine (10-20 mg/kg; i.p.; 30 min before LPS injection) reduces mortality, ameliorates lung injury, inhibits inflammatory cell infiltration, protein leakage, TNF-α, IL-6 production and NF-κB activation in LPS-induced acute lung injury in rats[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Sprague-Dawley rats (220-250 g) with Carrageenan-induced paw edema; Male Kunming mice (20-25 g) with Xylene-induced ear edema and LPS-induced systemic inflammation[1]
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Dosage:7 mg/kg, 21 mg/kg (for Carrageenan); 10 mg/kg, 30 mg/kg (for Xylene); 10 mg/kg, 20 mg/kg, 30 mg/kg (for LPS)
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Administration:i.p., 30 min before Carrageenan injection (for Carrageenan); i.p. (for Xylene); i.p., at 12 h and 1 h before LPS injection (for LPS)
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Result:Reduced paw edema (56.25% and 75.35% inhibition at 5 h) and TNF-α, IL-6 in paw tissue.
Decreased ear edema weight.
Inhibited serum TNF-α in a dose- and time-dependent manner.
Chemical Information
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CAS No. 4312-32-7
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Appearance Solid
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Molecular Weight 323.34
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Formula C19H17NO4
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Color White to off-white
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SMILES
C1(C(CCN2CC3=C4OCOC4=CC=C3CC21)=C5)=CC6=C5OCO6
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Synonyms
Tetrahydrocoptisine
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (2)
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Journal Impact Factor
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Most Recent
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BMC Complement Med Ther
Hepatotoxicity prediction for traditional Chinese medicine: a two-step in silico framework integrating network and machine learning approaches. [Abstract]2026 Apr 2;26(1):177. PMID: 41923057 -
Planta Med
Novel Approaches for the Analysis and Isolation of Benzylisoquinoline Alkaloids in Chelidonium majus. [Abstract]2024 Jun;90(7-08):523-533. PMID: 38843792
Solvent & Solubility
DMF : 4 mg/mL (12.37 mM; ultrasonic and warming and heat to 60°C)
DMSO : 2.5 mg/mL (7.73 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Acetone : 1 mg/mL (3.09 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 20% HP-β-CD in Saline
Solubility: 12.5 mg/mL (38.66 mM); Suspended solution; Need ultrasonic
Purity & Documentation
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Li W, et al. Anti-inflammatory effect of tetrahydrocoptisine from Corydalis impatiens is a function of possible inhibition of TNF-α, IL-6 and NO production in lipopolysaccharide-stimulated peritoneal macrophages through inhibiting NF-κB activation and MAPK pathway. Eur J Pharmacol. 2013 Sep 5;715(1-3):62-71. [Content Brief]
[2]. Li W, et al. Protective effect of tetrahydrocoptisine against ethanol-induced gastric ulcer in mice. Toxicol Appl Pharmacol. 2013 Oct 1;272(1):21-9. [Content Brief]
[3]. Khatun A, et al. In-Silico and In-Vivo Characterization of Anti-Neuro inflammatory potential of Tetrahydrocoptisine by using LPS-Induced model in mice. Neuroscience. 2025 Jan 26;565:232-246. [Content Brief]
[4]. Li W, et al. Tetrahydrocoptisine protects rats from LPS-induced acute lung injury. Inflammation. 2014 Dec;37(6):2106-15. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Acetone / DMSO / DMF | 1 mM | 3.0927 mL | 15.4636 mL | 30.9272 mL | 77.3180 mL |
| DMSO / DMF | 5 mM | 0.6185 mL | 3.0927 mL | 6.1854 mL | 15.4636 mL |
| DMF | 10 mM | 0.3093 mL | 1.5464 mL | 3.0927 mL | 7.7318 mL |
(±)-Stylopine Related Classifications
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