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  4. Ustekinumab

Ustekinumab  (Synonyms: CNTO-1275; L04AC05; TT-20)

Cat. No.: HY-P9909 Purity: 99.83%
COA
SDS
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Ustekinumab is an anti-IL-12/IL-23 IgG1κ human monoclonal antibody.

For research use only. We do not sell to patients.

CAS No. : 815610-63-0

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Customer Review

Based on 4 publication(s) in Google Scholar

Ustekinumab Featured Recommendations:

Ustekinumab Related Antibodies

Top Publications Citing Use of Products
Cell Proliferation/Viability Assay
In Vivo Efficacy Study
In Vivo Imaging

    Ustekinumab purchased from MedChemExpress. Usage Cited in: Adv Mater. 2024 Dec 6:e2412429.  [Abstract]

    Cascade repair probiotic gene editing system improves DSS-induced UC compared with clinical drugs. Daily change in body weight for each group, including Health, Control, 5-ASA (5-Aminosalicylic acid; 40 mg/kg; oral), vedolizumab (5 mg/kg; iv), ustekinumab (5 mg/kg; iv), infliximab (5 mg/kg; iv), and LGG-M@P, over 10 days. The above drugs were administered once every two days. Measurements of colonic tissue length in various groups on day 10. DAI changes throughout 10 days, calculated as the total of the stool consistency (0–3), fecal bleeding (0–4), and weight loss (0–4) indexes.

    Ustekinumab purchased from MedChemExpress. Usage Cited in: Adv Mater. 2024 Dec 6:e2412429.  [Abstract]

    Cascade repair probiotic gene editing system improves DSS-induced UC compared with clinical drugs. The groups are: Health, Control, 5-ASA (5-Aminosalicylic acid; 40 mg/kg; oral), vedolizumab (5 mg/kg; iv), ustekinumab (5 mg/kg; iv), infliximab (5 mg/kg; iv), and LGG-M@P, over 10 days. The above drugs were administered once every two days. Mice colonic sections stained with HE on day 10 following the indicated treatments.

    Ustekinumab purchased from MedChemExpress. Usage Cited in: Adv Mater. 2024 Dec 6:e2412429.  [Abstract]

    Cascade repair probiotic gene editing system improves DSS-induced UC compared with clinical drugs. The groups are: Health, Control, 5-ASA (5-Aminosalicylic acid; 40 mg/kg; oral), vedolizumab (5 mg/kg; iv), ustekinumab (5 mg/kg; iv), infliximab (5 mg/kg; iv), and LGG-M@P, over 10 days. The above drugs were administered once every two days. Colonic MPO levels on day 10.) Colonic TNF-α, IL-6, and IL-1β levels on day 10.

    Ustekinumab purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2021 Sep;22(9):1107-1117.  [Abstract]

    Flow cytometry quantification of cell viability and lipid ROS of HC neutrophils cultured in vitro with 20% SLE serum supplemented with anti-CXCL11 (0.1, 1, 5 µg/ml) or Ustekinumab (0.1, 1, 10 µg/ml) for 16 hours.

    View All Interleukin Related Isoform Specific Products:

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    IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Ustekinumab is an anti-IL-12/IL-23 IgG1κ human monoclonal antibody.

    Isotype

    Human IgG1 kappa
    Recommend Isotype Controls
    Species Reactivity

    Human
    IC50 & Target

    IL-12

     

    IL-23

     

    In Vitro

    Ustekinumab is a human monoclonal antibody that prevents human IL-12 and IL-23 from binding to the IL-12Rβ1 receptor chain of IL-12 (IL-12Rβ1/β2) and IL-23 (IL-12Rβ1/23R) receptor complexes on the surface of natural killer cells and T-cells[1]. Ustekinumab is a human IgG1 kappa (κ) monoclonal antibody against the IL-12/IL-23 p40 subunit that prevents its interaction with IL-12Rβ1, thereby blocking subsequent signaling. The FcRn affinities at pH 6.0 fell in a narrow range for all 11 antibodies. The equilibrium dissociation constant (KD) is calculated relative to Ustekinumab (Ustekinumab=1.0)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Ustekinumab Related Antibodies
    Clinical Trial
    Gene ID

    3593  [NCBI]

    Accession
    Application

    ELISA, FACS, Functional assay
    Conjugated

    Unconjugated
    Reconsititution

    The product can be reconstituted/diluted with sterile PBS or saline.
    Molecular Weight

    145.63 kDa

    CAS No.
    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    [Ustekinumab]
    Shipping

    Shipping with dry ice.
    Formulation

    Please refer to the lot-specific COA for specific buffer information.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Format
    • Human IgG1 kappa
    Biological Activity
    • Immobilized IL-12 beta Protein, Human (HEK293, Fc, HY-P73160) can bind Ustekinumab. The EC50 for this effect is 13.6 ng/mL.
    • Flow cytometric analysis of 1×106 Jurkat cells labelling IL-12B (red) with Ustekinumab (HY-P9909). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa Isotype Control (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
    Purity & Documentation

    Purity: 99.83%

    SDS (394 KB)

    COA (232 KB) SDS-PAGE (109 KB) SEC-HPLC (86 KB)

    Inhibitory Antibodies User Guide (603 KB)
    References
    Cell Assay
    [2]

    HUVECs are grown on calibrated glass coverslips to a confluency of 80%. Subsequently, cells are incubated with 200 µg/mL Ustekinumab AlexaFluor488 and Briakinumab AlexaFluor594 for 20 min at 37°C, pH 7.3, washed three times, and chased in fresh medium for an additional 20 min before fixation. Antibodies are directly labeled. FcRn binding is quality controlled by SPR analysis. Cells are then counterstained with anti-FcRn monoclonal antibody DVN22, followed by anti-mouse secondary antibodies conjugated to AlexaFluor647. Imaging is performed on a Leica SP8 confocal microscope[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
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    Ustekinumab Related Classifications

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ustekinumab815610-63-0CNTO-1275 L04AC05 TT-20CNTO1275CNTO 1275TT20TT 20TT-20Interleukin RelatedILInhibitorinhibitorinhibit

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