AMG-966 

AMG-966 is a human bispecific antibody (bsAb) targeting TNFSF15/TL1A & TNFα. AMG 966 restores the ability of the aglycosylated Fc domain to bind FcγRIa and FcγRIIa, leading to the formation of anti-drug antibodies (ADA). AMG 966 can be used in research on Crohn's disease and ulcerative colitis. Recommended isotype control: Human IgG1 kappa, Isotype Control (HY-P99001)^[1].

Human IgG1 kappa

Human IgG1 kappa, Isotype Control

Human

TNFSF15

AMG-966 (300 nM; 6 days) elicits minimal CD4+ T cell proliferation in a human DC:T cell assay from 30 global HLA-representative donors, with no impact from its engineered charge pair mutations^[1].
AMG-966 (1:1, 1:1:1, 9:1:1, 200:1:1, 600:1:1 molar ratios; approximately 16 hours) forms intermediate-sized immune complexes with recombinant human TNF at a 1:1 ratio and larger complexes when combined with both TNF and TL1A at lower drug-to-target ratios, as measured by SEC-MALS after 16-hour incubation at 4°C^[1].
AMG-966 (1:1:1, 4:1:1, 9:1:1, 100:1:1 molar ratios; overnight) forms immune complexes with recombinant human TNF and TL1A that exhibit enhanced binding to FcγRIa and FcγRIIa at low 1:1:1 drug-to-target ratios (after overnight incubation at 4°C), with binding decreasing as the drug-to-target ratio increases, while FcγRIIIA binding is minimally affected^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9966  [NCBI]

O95150

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

145.25 kDa

Liquid

Colorless to light yellow

[AMG-966]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Flow cytometric analysis of 1×10⁶ MCF-7 cells labelling TL1A (red) with AMG-966 (HY-P991440). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa Isotype Control (HY-P99001, blue) was used as the isotype control.

• [1]. Kroenke MA, et al. Immune Complex Formation Is Associated With Loss of Tolerance and an Antibody Response to Both Drug and Target. Front Immunol. 2021;12:782788. Published 2021 Dec 14.  [Content Brief]