Annonacin
Based on 1 publication(s) in Google Scholar
Annonacin is an acetylgenin that is toxic by inhibiting the pathway of the mitochondrial complex. Annonacin increases tau phosphorylation in R406W+/+ mice. Annonacin acts as an inhibitor of the sodium/potassium and sarcoplasmic reticulum (SERCA) ATPase pumps. Annonacin has significant killing effect on ovarian cancer cell, cervical cancer cell, breast cancer cell, bladder cancer cell and skin cancer cell. Annonacin induces apoptosis through Bax and Caspase-3-related pathways.
For research use only. We do not sell to patients.
- Purity: 98.09%
- CAS No.: 111035-65-5
- Formula: C35H64O7
- Molecular Weight:596.88
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Annonacin
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Biological Activity
Annonacin (1-10 μg/mL; 0-72 h) induces the death of a variety of cancer cell lines, and blocks cancer cells in G1 phase [2].
Annonacin 10 μg/mL; 0-24 h) induces apoptosis of T24 cells and enhance the expression of pro-apoptosis-related genes [2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:T24 cells
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Concentration:1 μg/mL
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Incubation Time:0, 24, 48 and 72 h
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Result:Significantly reduced cell viability. Compared with untreated controls, only 56.0%, 34.2% and 35.3% of T24 cells were alive at 24, 48 and 72 hours, respectively.
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Cell Line:T24 cells
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Concentration:10 μg/mL
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Incubation Time:1, 4, 8, 12 and 24 h
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Result:Increased the levels of Bax and Bad.
Inhibited the levels of procaspase-3.
Annonacin (85 nM/100 μL; Topical application; Twice a week for 22 weeks) has anti-cancer effect in mouse model of skin tumor [4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:R406W+/+ transgenic mice[3]
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Dosage:0, 6 and 9 mg/kg/day
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Administration:Subcutaneous osmotic pump; 3 days
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Result:Increased the number of neurons with phosphorylated tau in the somatodendritic compartment in several brain areas.
Increased the levels of phosphorylated tau protein.
Increased in total tau protein without increase in tau mRNA, but reduced proteasomal proteolytic activity.
Increased the levels of different tau kinases.
Significantly increased the ratio of p25/p35.
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Animal Model:DMBA (HY-W011845)/TPA treated female ICR mice aged 6-7 weeks old[4]
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Dosage:85 nM/100 uL
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Administration:Topical application; Twice a week for 22 weeks
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Result:Significantly increased the tumor latency period and reduced the tumor incidence, tumor burden and tumor volume, respectively.
Suppressed tumorigenesis manifested by significant reduction of hyperkeratosis, dermal papillae and number of keratin pearls on skin tissues.
Showed no toxicity to the liver and kidneys.
Chemical Information
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CAS No. 111035-65-5
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Appearance Solid
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Molecular Weight 596.88
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Formula C35H64O7
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Color White to off-white
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SMILES
O=C1O[C@@H](C)C=C1C[C@H](O)CCCCC[C@@H](O)CCCC[C@@H](O)[C@@H]2O[C@@H]([C@H](O)CCCCCCCCCCCC)CC2
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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J Cell Mol Med
Anticancer Activity of Annonacin and Its Synergistic Enhancement of Docetaxel Efficacy in Prostate Cancer. [Abstract]2025 Dec;29(23):e70972. PMID: 41328625
Solvent & Solubility
DMSO : 100 mg/mL (167.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Yiallouris A, et al. Annonacin promotes selective cancer cell death via NKA-dependent and SERCA-dependent pathways. Cell Death Dis. 2018 Jul 9;9(7):764. [Content Brief]
[2]. Yuan SS, et al. Annonacin, a mono-tetrahydrofuran acetogenin, arrests cancer cells at the G1 phase and causes cytotoxicity in a Bax- and caspase-3-related pathway. Life Sci. 2003 May 9;72(25):2853-61. [Content Brief]
[3]. Yamada ES, et al. Annonacin, a natural lipophilic mitochondrial complex I inhibitor, increases phosphorylation of tau in the brain of FTDP-17 transgenic mice. Exp Neurol. 2014 Mar;253:113-25. [Content Brief]
[4]. Md Roduan MR, et al. Modulation of cancer signalling pathway(s) in two -stage mouse skin tumorigenesis by annonacin. BMC Complement Altern Med. 2019 Sep 3;19(1):238. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.6754 mL | 8.3769 mL | 16.7538 mL | 41.8845 mL |
| 5 mM | 0.3351 mL | 1.6754 mL | 3.3508 mL | 8.3769 mL | |
| 10 mM | 0.1675 mL | 0.8377 mL | 1.6754 mL | 4.1884 mL | |
| 15 mM | 0.1117 mL | 0.5585 mL | 1.1169 mL | 2.7923 mL | |
| 20 mM | 0.0838 mL | 0.4188 mL | 0.8377 mL | 2.0942 mL | |
| 25 mM | 0.0670 mL | 0.3351 mL | 0.6702 mL | 1.6754 mL | |
| 30 mM | 0.0558 mL | 0.2792 mL | 0.5585 mL | 1.3961 mL | |
| 40 mM | 0.0419 mL | 0.2094 mL | 0.4188 mL | 1.0471 mL | |
| 50 mM | 0.0335 mL | 0.1675 mL | 0.3351 mL | 0.8377 mL | |
| 60 mM | 0.0279 mL | 0.1396 mL | 0.2792 mL | 0.6981 mL | |
| 80 mM | 0.0209 mL | 0.1047 mL | 0.2094 mL | 0.5236 mL | |
| 100 mM | 0.0168 mL | 0.0838 mL | 0.1675 mL | 0.4188 mL |
Annonacin Related Classifications
HY-N2877 Related Classifications
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